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The map label for this gene is ppa [H]

Identifier: 229586928

GI number: 229586928

Start: 851642

End: 852163

Strand: Direct

Name: ppa [H]

Synonym: RAF_ORF0814

Alternate gene names: 229586928

Gene position: 851642-852163 (Clockwise)

Preceding gene: 229586927

Following gene: 229586929

Centisome position: 66.61

GC content: 36.97

Gene sequence:

>522_bases
ATGTTTATAGATAAAATCAAAGCAAAAGCAAATAATTACGAAATAAACGTAATTATCGAAATTCCGATGAATAGCGGTCC
AATCAAATACGAATGTGACAAAGAATCAGGAGCAATTTTTGTTGATCGCTTTATGCAAACTACCATGAGCTATCCATGCA
ATTACGGTTTTATTCCTCATACTCTTTCAAATGACGGTGATCCGATAGACGTACTTGTCGTAGCTCATCATCCGGTAGTG
CCTGGGTCGGTTATAAAATGCCGAGCAGTCGGTGTATTGATGATGGAAGACGAATCAGGGCTTGATGAGAAAATAATCGC
AGTACCGACTTCTAAGCTCGATATTACTTTCGATCATATTAAAGAGCTAGATGATTTATGTGACATGCTCAAGCAACGTA
TCGTGCATTTCTTTGAGCATTATAAAGATTTAGAAAAAGGTAAATGGGTTAAAGTTACTGGATGGGAAAATAAATCAAAA
GCAGACGCTTTAATCAATGAAGGAATAGATAGGGTCAGCTAG

Upstream 100 bases:

>100_bases
ACGTGGCAATCTAGTAAAATAGGCCTGAGATTGCTTCGTCGAATTACGTTGTAATTCTCCTCGCAATGACAAAAAATAAC
TAAACCAAATAAATAAAAAC

Downstream 100 bases:

>100_bases
GGATAAGAATTCATGACGTTGCTTGCATGGTGCTTGATGTCATTCCCGCGGAGGCGGGAATCCAGAAAAAGTAGCCTTAA
TCCCGATCTCAACAATTACA

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MFIDKIKAKANNYEINVIIEIPMNSGPIKYECDKESGAIFVDRFMQTTMSYPCNYGFIPHTLSNDGDPIDVLVVAHHPVV
PGSVIKCRAVGVLMMEDESGLDEKIIAVPTSKLDITFDHIKELDDLCDMLKQRIVHFFEHYKDLEKGKWVKVTGWENKSK
ADALINEGIDRVS

Sequences:

>Translated_173_residues
MFIDKIKAKANNYEINVIIEIPMNSGPIKYECDKESGAIFVDRFMQTTMSYPCNYGFIPHTLSNDGDPIDVLVVAHHPVV
PGSVIKCRAVGVLMMEDESGLDEKIIAVPTSKLDITFDHIKELDDLCDMLKQRIVHFFEHYKDLEKGKWVKVTGWENKSK
ADALINEGIDRVS
>Mature_173_residues
MFIDKIKAKANNYEINVIIEIPMNSGPIKYECDKESGAIFVDRFMQTTMSYPCNYGFIPHTLSNDGDPIDVLVVAHHPVV
PGSVIKCRAVGVLMMEDESGLDEKIIAVPTSKLDITFDHIKELDDLCDMLKQRIVHFFEHYKDLEKGKWVKVTGWENKSK
ADALINEGIDRVS

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=159, Percent_Identity=64.7798742138365, Blast_Score=219, Evalue=9e-59,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19588; Mature: 19588

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIDKIKAKANNYEINVIIEIPMNSGPIKYECDKESGAIFVDRFMQTTMSYPCNYGFIPH
CCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC
TLSNDGDPIDVLVVAHHPVVPGSVIKCRAVGVLMMEDESGLDEKIIAVPTSKLDITFDHI
CCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCCEEEEHHHH
KELDDLCDMLKQRIVHFFEHYKDLEKGKWVKVTGWENKSKADALINEGIDRVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHCCC
>Mature Secondary Structure
MFIDKIKAKANNYEINVIIEIPMNSGPIKYECDKESGAIFVDRFMQTTMSYPCNYGFIPH
CCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC
TLSNDGDPIDVLVVAHHPVVPGSVIKCRAVGVLMMEDESGLDEKIIAVPTSKLDITFDHI
CCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCCEEEEHHHH
KELDDLCDMLKQRIVHFFEHYKDLEKGKWVKVTGWENKSKADALINEGIDRVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA