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The map label for this gene is ntrX [C]

Identifier: 229586894

GI number: 229586894

Start: 807044

End: 808468

Strand: Direct

Name: ntrX [C]

Synonym: RAF_ORF0773

Alternate gene names: 229586894

Gene position: 807044-808468 (Clockwise)

Preceding gene: 229586888

Following gene: 229586895

Centisome position: 63.12

GC content: 32.21

Gene sequence:

>1425_bases
ATGTCACAACTAGATGTTTTAATAGTAGACGATGAAGAGAGTATACGAAATCTCATTGCTGCAAATTTGAAAGATGAAGG
TTTTAACCCTAAGGTTGCCGCTAATAGTACTCAAGCCCTTAAAATACTTTCCGAAAAACCGGTCTCTGCAGTTATACTTG
ATATTTGGCTTCAAGGAAGCGAAATTGACGGACTTGGGATTTTAGAGATAATAAAAAAACGCTATCCTTTAATGCCGGTA
ATAATTATTAGCGGTCACGGTACTATAGAAACAGCAGTAAATGCTATAAAAATGGGTGCTTACGATTATATAGAAAAACC
GTTTAATAATGATAAATTAGTTATTTTACTTACAAGAGCTTGTGAAGTAACAAAGTTAAAACGCGAAAATATAGATTTAA
AATCAAAGGTTATAGATAAAACTGAATTAGTAGGCGAATGTTCGGTAACTTTAAAATATAAAATGGCAATAGCAAAGGCA
GCTACCTCTAGCAGTCGTATAATGATTCACGGTAAAGTCGGTAGCGGTAAAGAACTTGCAGCAAGATTAATTCATAAACA
ATCTAAAAGGGTTAATAATCCGTTCATTATTTTCAGCCCTACCTGTATGACTACGGAAAAAATCAATCAAGAATTATTCG
GCGAATCGGAAAAGCAGGCAAATAATAAACGCCCTACTATCTTAGAATTTGCCAATAACGGTACTTTATATATAGATGAG
GTCAGTAATATTCCTATTCCCATCCAAGTAAAATTATTAAAATTCCTTAAAGATCAAACTATTACAAAACCTTGCGGAAA
AAATATTAAAATTGATATAAAAATTATCACCGGTACTTCTAAAAATATCCAAGATGAAGTTAATAACGGCAAATTCCTAG
AAGATCTATATTATCGCCTTAATGTATCCTCTCTAAAAGTACCTTCATTATATGAAAGAAAAGAAGATATACCGCTACTC
GTTAAGTATTTTGTTAAGCAACTTTCAAAATTTTCAGGTTTAAAAGAACGTAGCTTTGCCGATGAAACTATCGCTGCTCT
TCAATCCTATGAATGGCCGGGCAATATTAGACAATTACGTAACGTTGTTGAATGGACTTTAATTATGAATCCGTTAACTA
CAGGTAATAATGAAATTATAAAACCTTATATGATACCTTCTGAAATATTAGCAAATAGTGCTAATCTTACAAAACTTGAA
GATAGCTTTGATATGTTATCTATGCCGCTTAGAGAAGCTAGAGAAGTTTTTGAGCGTCAATATCTATCGGCACAAATGAG
TCGTTTTAATAATAATATTTCAAAAACATCCTCATTTGTCGGTATGGAAAGATCGGCTTTGCATCGTAAATTAAAATTAT
TAAGCTTACATATACCTCCTACAAATAGAATAAATGACGAAGAATATGAGGAAGCAAATGCTTAA

Upstream 100 bases:

>100_bases
CTAATTCTTTCAAAAATCTTATTAATTATCGCTAAAAAATATCAATCAATTAGCAATCGTACGCAAGAACCTAGTATTAT
TATTTTTAAGGTAAATTGTA

Downstream 100 bases:

>100_bases
AGTCATATCAATAATTACGATTTATTTGTTATTAAGCAGCTGTTCCGAATCTACCCGTGATGCGAACGGATTACTTACGG
ATAGCCAAAGCACGGTAATT

Product: Nitrogen assimilation regulatory protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSQLDVLIVDDEESIRNLIAANLKDEGFNPKVAANSTQALKILSEKPVSAVILDIWLQGSEIDGLGILEIIKKRYPLMPV
IIISGHGTIETAVNAIKMGAYDYIEKPFNNDKLVILLTRACEVTKLKRENIDLKSKVIDKTELVGECSVTLKYKMAIAKA
ATSSSRIMIHGKVGSGKELAARLIHKQSKRVNNPFIIFSPTCMTTEKINQELFGESEKQANNKRPTILEFANNGTLYIDE
VSNIPIPIQVKLLKFLKDQTITKPCGKNIKIDIKIITGTSKNIQDEVNNGKFLEDLYYRLNVSSLKVPSLYERKEDIPLL
VKYFVKQLSKFSGLKERSFADETIAALQSYEWPGNIRQLRNVVEWTLIMNPLTTGNNEIIKPYMIPSEILANSANLTKLE
DSFDMLSMPLREAREVFERQYLSAQMSRFNNNISKTSSFVGMERSALHRKLKLLSLHIPPTNRINDEEYEEANA

Sequences:

>Translated_474_residues
MSQLDVLIVDDEESIRNLIAANLKDEGFNPKVAANSTQALKILSEKPVSAVILDIWLQGSEIDGLGILEIIKKRYPLMPV
IIISGHGTIETAVNAIKMGAYDYIEKPFNNDKLVILLTRACEVTKLKRENIDLKSKVIDKTELVGECSVTLKYKMAIAKA
ATSSSRIMIHGKVGSGKELAARLIHKQSKRVNNPFIIFSPTCMTTEKINQELFGESEKQANNKRPTILEFANNGTLYIDE
VSNIPIPIQVKLLKFLKDQTITKPCGKNIKIDIKIITGTSKNIQDEVNNGKFLEDLYYRLNVSSLKVPSLYERKEDIPLL
VKYFVKQLSKFSGLKERSFADETIAALQSYEWPGNIRQLRNVVEWTLIMNPLTTGNNEIIKPYMIPSEILANSANLTKLE
DSFDMLSMPLREAREVFERQYLSAQMSRFNNNISKTSSFVGMERSALHRKLKLLSLHIPPTNRINDEEYEEANA
>Mature_473_residues
SQLDVLIVDDEESIRNLIAANLKDEGFNPKVAANSTQALKILSEKPVSAVILDIWLQGSEIDGLGILEIIKKRYPLMPVI
IISGHGTIETAVNAIKMGAYDYIEKPFNNDKLVILLTRACEVTKLKRENIDLKSKVIDKTELVGECSVTLKYKMAIAKAA
TSSSRIMIHGKVGSGKELAARLIHKQSKRVNNPFIIFSPTCMTTEKINQELFGESEKQANNKRPTILEFANNGTLYIDEV
SNIPIPIQVKLLKFLKDQTITKPCGKNIKIDIKIITGTSKNIQDEVNNGKFLEDLYYRLNVSSLKVPSLYERKEDIPLLV
KYFVKQLSKFSGLKERSFADETIAALQSYEWPGNIRQLRNVVEWTLIMNPLTTGNNEIIKPYMIPSEILANSANLTKLED
SFDMLSMPLREAREVFERQYLSAQMSRFNNNISKTSSFVGMERSALHRKLKLLSLHIPPTNRINDEEYEEANA

Specific function: Member of the two-component regulatory system RBE_0312/RBE_0470 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=32.1353065539112, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1790437, Length=455, Percent_Identity=32.5274725274725, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=32.7510917030568, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=30.68669527897, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=34.2679127725857, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=28.3536585365854, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082117, Length=306, Percent_Identity=31.0457516339869, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789087, Length=356, Percent_Identity=31.1797752808989, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786524, Length=336, Percent_Identity=29.1666666666667, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=30.3225806451613, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=29.5522388059701, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=29.2929292929293, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789828, Length=214, Percent_Identity=29.9065420560748, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=32.1100917431193, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53540; Mature: 53409

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLDVLIVDDEESIRNLIAANLKDEGFNPKVAANSTQALKILSEKPVSAVILDIWLQGS
CCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCHHEEEEEEEECCC
EIDGLGILEIIKKRYPLMPVIIISGHGTIETAVNAIKMGAYDYIEKPFNNDKLVILLTRA
CCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECC
CEVTKLKRENIDLKSKVIDKTELVGECSVTLKYKMAIAKAATSSSRIMIHGKVGSGKELA
CHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEEEHHHHHCCCCCEEEEEECCCCCHHHH
ARLIHKQSKRVNNPFIIFSPTCMTTEKINQELFGESEKQANNKRPTILEFANNGTLYIDE
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCEEEEEECCCEEEEEE
VSNIPIPIQVKLLKFLKDQTITKPCGKNIKIDIKIITGTSKNIQDEVNNGKFLEDLYYRL
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHCCCCHHHHHHHHHC
NVSSLKVPSLYERKEDIPLLVKYFVKQLSKFSGLKERSFADETIAALQSYEWPGNIRQLR
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
NVVEWTLIMNPLTTGNNEIIKPYMIPSEILANSANLTKLEDSFDMLSMPLREAREVFERQ
HHHHHHHEECCCCCCCCCEEEEEECCHHHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHHH
YLSAQMSRFNNNISKTSSFVGMERSALHRKLKLLSLHIPPTNRINDEEYEEANA
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEEEEEEECCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SQLDVLIVDDEESIRNLIAANLKDEGFNPKVAANSTQALKILSEKPVSAVILDIWLQGS
CCEEEEEECCHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCHHEEEEEEEECCC
EIDGLGILEIIKKRYPLMPVIIISGHGTIETAVNAIKMGAYDYIEKPFNNDKLVILLTRA
CCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECC
CEVTKLKRENIDLKSKVIDKTELVGECSVTLKYKMAIAKAATSSSRIMIHGKVGSGKELA
CHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEEEHHHHHCCCCCEEEEEECCCCCHHHH
ARLIHKQSKRVNNPFIIFSPTCMTTEKINQELFGESEKQANNKRPTILEFANNGTLYIDE
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCEEEEEECCCEEEEEE
VSNIPIPIQVKLLKFLKDQTITKPCGKNIKIDIKIITGTSKNIQDEVNNGKFLEDLYYRL
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHCCCCHHHHHHHHHC
NVSSLKVPSLYERKEDIPLLVKYFVKQLSKFSGLKERSFADETIAALQSYEWPGNIRQLR
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
NVVEWTLIMNPLTTGNNEIIKPYMIPSEILANSANLTKLEDSFDMLSMPLREAREVFERQ
HHHHHHHEECCCCCCCCCEEEEEECCHHHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHHH
YLSAQMSRFNNNISKTSSFVGMERSALHRKLKLLSLHIPPTNRINDEEYEEANA
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEEEEEEECCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA