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The map label for this gene is ilvE [H]

Identifier: 229586706

GI number: 229586706

Start: 582714

End: 583586

Strand: Direct

Name: ilvE [H]

Synonym: RAF_ORF0553

Alternate gene names: 229586706

Gene position: 582714-583586 (Clockwise)

Preceding gene: 229586699

Following gene: 229586707

Centisome position: 45.58

GC content: 34.25

Gene sequence:

>873_bases
ATGACAATGGTAAAACTAGAGCAAATTTTTGGGCATGTTTGGATTAACGGTGATTTAGTTCCTTATCAATTTGCAAGGAT
TCATGTTTTGACTCATAGCCTGCATTATTCAGGATCGGTATTTGAGGGTGAGAGAGCTTATAACGGCAAAGTTTTTAAAC
TAAAAGAACATACGGCAAGATTAATAAAATCAGCAGAAGCATTAGGTTTGAAAGTACCTTATAATGTAGACGAGATAATA
AAGGCTCATGAACGTGTAATAAAACAAAATAATATTAAGGATGCATATATAAGACCGCTTATTTGGTGCGGAGACGAGTC
GCTAAATATCACTAATCAGTATTTATCTACTAATCTTTTGATTGCCGGTATTCCATCTATGCCGAGGTCTTTTGAGAAAG
GTATAAATTTGCATGTAAGCCGCTGGCGTAAAGCAATGCCTGATAGCACCCCGGTACAATCTAAATCCGCCGCTCAATAT
AATATGGCAATAACGAGTAAAAAAGAAGCTAAGGCTCTTGGCTATGAGGATGCATTATTACTTGATTATGAAGGGTATAT
TGCTGAATGCACTACAACAAATATTTTCTTTGTTAAAGATAAAATATTATATACTCCAATTGCCGATAGGTTTTTAAACG
GTATAACAAGACAGACTATTATTGAAATTGCAAAAGATTTAGGTCTAGAAGTAAAAGAAGAGCGTTTAAAATTAGAGCAA
ATAGAGGATTTTACCGGATGTTTTGTTACAGGCACAGCAATTGAGGTACAAAATATTGATTCTATAGATCTTGGCAATAA
AAAGATTATTTTTGATGATCATAAAATTGCAGATCGTTTGAAAGAAGAGTATAGGCGTGTTGTTAGGGAGTAG

Upstream 100 bases:

>100_bases
CGTTAAATACTACTGGATTCCCGCTTTCGCAGGAATGACATAAGAACTACGGCCGTATAGCTCATGACGATTGACAATAC
TTTAATATAAATATAGGTTA

Downstream 100 bases:

>100_bases
AAGAAACGTCATTGCGAGGAGGTATTGCCTGTGTATGGATACCGAGTCGTCATTGCGAGCAGTCGTAGACTGCGTGGCAA
TCTCATGAAATAATAACAAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTMVKLEQIFGHVWINGDLVPYQFARIHVLTHSLHYSGSVFEGERAYNGKVFKLKEHTARLIKSAEALGLKVPYNVDEII
KAHERVIKQNNIKDAYIRPLIWCGDESLNITNQYLSTNLLIAGIPSMPRSFEKGINLHVSRWRKAMPDSTPVQSKSAAQY
NMAITSKKEAKALGYEDALLLDYEGYIAECTTTNIFFVKDKILYTPIADRFLNGITRQTIIEIAKDLGLEVKEERLKLEQ
IEDFTGCFVTGTAIEVQNIDSIDLGNKKIIFDDHKIADRLKEEYRRVVRE

Sequences:

>Translated_290_residues
MTMVKLEQIFGHVWINGDLVPYQFARIHVLTHSLHYSGSVFEGERAYNGKVFKLKEHTARLIKSAEALGLKVPYNVDEII
KAHERVIKQNNIKDAYIRPLIWCGDESLNITNQYLSTNLLIAGIPSMPRSFEKGINLHVSRWRKAMPDSTPVQSKSAAQY
NMAITSKKEAKALGYEDALLLDYEGYIAECTTTNIFFVKDKILYTPIADRFLNGITRQTIIEIAKDLGLEVKEERLKLEQ
IEDFTGCFVTGTAIEVQNIDSIDLGNKKIIFDDHKIADRLKEEYRRVVRE
>Mature_289_residues
TMVKLEQIFGHVWINGDLVPYQFARIHVLTHSLHYSGSVFEGERAYNGKVFKLKEHTARLIKSAEALGLKVPYNVDEIIK
AHERVIKQNNIKDAYIRPLIWCGDESLNITNQYLSTNLLIAGIPSMPRSFEKGINLHVSRWRKAMPDSTPVQSKSAAQYN
MAITSKKEAKALGYEDALLLDYEGYIAECTTTNIFFVKDKILYTPIADRFLNGITRQTIIEIAKDLGLEVKEERLKLEQI
EDFTGCFVTGTAIEVQNIDSIDLGNKKIIFDDHKIADRLKEEYRRVVRE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=262, Percent_Identity=38.1679389312977, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17565728, Length=290, Percent_Identity=25.5172413793103, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33168; Mature: 33036

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMVKLEQIFGHVWINGDLVPYQFARIHVLTHSLHYSGSVFEGERAYNGKVFKLKEHTAR
CCEEEEEHHHCEEEECCCCCCHHHHEEEEEEHHHCCCCCEECCCCCCCCEEEEEHHHHHH
LIKSAEALGLKVPYNVDEIIKAHERVIKQNNIKDAYIRPLIWCGDESLNITNQYLSTNLL
HHHHHHHCCEECCCCHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCHHHHHHHCCEE
IAGIPSMPRSFEKGINLHVSRWRKAMPDSTPVQSKSAAQYNMAITSKKEAKALGYEDALL
EEECCCCCHHHHHCCCEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEE
LDYEGYIAECTTTNIFFVKDKILYTPIADRFLNGITRQTIIEIAKDLGLEVKEERLKLEQ
EECCCEEEEEEECEEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
IEDFTGCFVTGTAIEVQNIDSIDLGNKKIIFDDHKIADRLKEEYRRVVRE
HHHHCCEEEECCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMVKLEQIFGHVWINGDLVPYQFARIHVLTHSLHYSGSVFEGERAYNGKVFKLKEHTAR
CEEEEEHHHCEEEECCCCCCHHHHEEEEEEHHHCCCCCEECCCCCCCCEEEEEHHHHHH
LIKSAEALGLKVPYNVDEIIKAHERVIKQNNIKDAYIRPLIWCGDESLNITNQYLSTNLL
HHHHHHHCCEECCCCHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCHHHHHHHCCEE
IAGIPSMPRSFEKGINLHVSRWRKAMPDSTPVQSKSAAQYNMAITSKKEAKALGYEDALL
EEECCCCCHHHHHCCCEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEE
LDYEGYIAECTTTNIFFVKDKILYTPIADRFLNGITRQTIIEIAKDLGLEVKEERLKLEQ
EECCCEEEEEEECEEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
IEDFTGCFVTGTAIEVQNIDSIDLGNKKIIFDDHKIADRLKEEYRRVVRE
HHHHCCEEEECCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]