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The map label for this gene is leuS

Identifier: 229586699

GI number: 229586699

Start: 574277

End: 576784

Strand: Direct

Name: leuS

Synonym: RAF_ORF0546

Alternate gene names: 229586699

Gene position: 574277-576784 (Clockwise)

Preceding gene: 229586698

Following gene: 229586706

Centisome position: 44.92

GC content: 36.32

Gene sequence:

>2508_bases
ATGAACCAAATAGAACAAAAATGGCAGTACATTTGGCAAGAAGAAAAAGCTTTTGAGGTATCAAACGCGAGTAGCAAGCC
AAAATATTACGTACTTGAAATGCTGCCTTATCCTTCCGGTAAAATCCATGTAGGTCACGTACGCAACTATTCTATAGGTG
ATGTTATTGCAAGGTTTATGACTATGCAAGGTTTTAATGTGCTTCATCCGATGGGCTGGGATGCTTTCGGGCTACCTGCC
GAACATGCCGCAATAAAAAATAATTCTCACCCAAAAAAATGGACATATTCTAATATCAAGAATATGAAAAAGCAGCTAAA
ATCTATGGGCTTTTCTTATGATTGGTCTAGAGAGATAAATAGTTGTGATCCTGAGTATTATAAACATGAGCAGAAATTCT
TTTTAGAGCTTTATGAACGAAATCTTGCTTATCAAAAAGAATCATTTGTTAATTGGGATCCGGTTGATAATACCGTGCTT
GCGAATGAACAAGTTGTAGACGGGCGTGGTTGGCGTTCAGGTGCAATAGTTGTAAAACGTTATTTAAAGCAATGGTTCTT
AAAAATTACCGATTATGCTGAAGAATTATTAAACGAAATTCAGAATTTAAAAGAGTGGCCTGAAGCTGTTCGGTCTATGC
AAGAGAAATGGATAGGTAAATCGATAGGTGCTAATTTTCATTTCAAAATTAAGGATAATGAAGAGACTACTATAGAAGTA
TTCTCAACCAAACCTGAAACCATTTTTGGTGCTAGCTTTATAGGAATTGCCTTTAATCATCCGATAATAGAGCGGTTAGT
TTCTAAAACACCTGAAATACTCGCTTTTATAACTCAATGCTCGCATATTACAAGAAGTAGCGAACTAGAAAAGACTGAAA
GGGAAGGTGTATTTACCGGTCTTTTTGTTATTCATCCTTTCGATTCAAATATAGTTTTACCGGTTATTATTACTAATTTT
GTGCTAATGGATTATGGAACTGGTGCAATATTTGGTTGCCCTGCTCATGACGAATGTGACCATGAATTAGCTGTCAAAAT
GAATTTATCTATTAAACAAGTAATTAAAGCAGATATGGATGTACAAAAGACTGCTTATACGGAAGATGGAATTTTAATTA
ATTCGGATTTTCTAAACGGCTTAACTAGTCACGAAGCAAAACAAGAAGTCATCGGAGAATTTGAGAAACTAGGCATAGGT
AAACGCAGCGTAAATTATCGTCTAAAAGATTGGGGGATTTCAAGGCAGCGCTTTTGGGGATGTCCTATTCCTATGATTCA
CTGCGAAATTTGTGGCATAGTGCCTGTACCTTACAAAGACTTACCCGTTATTTTACCTGATGATGTTAATTTTGACGGTC
ACGGTAATCCGCTTGATCATCATCCTAGTTGGAAACATGTTAATTGTCCGAAATGCGATAAGCCGGCTGTGCGTGAAACC
GATACTTTTGATACATTTTTTGAATCATCTTGGTATTTTATGAGATATTGCAATAGCAATGCTACAGAGATGACTGATAA
AAAAGCTTGTGATTACTGGCTACCGGTCGATAAATATATAGGCGGCATTGAACATGCGGTAATGCATTTATTATATGCAA
GGTTTTTCACAAAAGTAATGAACGAACAGAATTACGTAAGCGTTCGGGAGCCTTTTAAAGGGCTATTTACTCAAGGTATG
GTGCTGCATGCCACTTACAAAGATGAACATAATAATTGGTTATATCCTGAAGAAGTAGTTAAAAAGGGTAATGAATTTTT
TCATAAAGAAAGCAATAATAGAGTAGTGCAGGGACGCATTGAAAAGATGAGTAAGTCTAAAAAGAATCTCATCGATCTTG
AGACAATGCAAGAGCAATATGGGGCAGATGCTATTAGGCTTTTTGTACTGTCCGATAGTCCGCCTGAGAAAGATCTAGAA
TGGTCGGCAAGCGGTATTGAAGGCTGCTCACGTTTTATTAATAAGCTTGAGTATATGTTTAAGGCGATAGATTCTTTAAA
AGATGATGTGAATAGCGAGGTTAATAAAGAGCTTAATAGGTTAGTACATTTTACAATAAAGCACGTCGCCGAAGATATAA
AGCATTTTGCCCTAAACAGAGCAATAGCACGTATGCGCGAGCTTTCTAATGCTATATCTGCTGAAATATCCAAAGATAAA
ATTGATGTTAAAACCGTAAGGCATGGCTTTAATGTTTTAGTACAGCTACTAAATCCTTTTATCCCGCATATTACGGAAGA
AATTTGGCAAAAGCTTGGCAATAAAGAGCGATTATATAATTTGTCTTTTCCTGCATTTGATGAATCAATGCTAGAGTTAG
ATACATATATTATGGCAGTGCAGGTTAACGGCAAACTCCGTGATACCTATGAGTTTAAAACTTCCGTAAGTGAAGATGAA
ATAAAGCAAGTTACCGTCAGTCTACCAAAGGTACAAAAGTTTTTAGAAGGGCAAGAGCCGAAGAAGATTATCCTCGTTCC
TCGTAAAATTGTGAATATACTCGTTTAA

Upstream 100 bases:

>100_bases
TTACGGAGTAATTGACAAAGCAATCTCAGGATATTTGATGAGATTGCCGTGCAGTCTATGACTGCTCGCAATGACAATGT
AGTATATAAATTAAAAAAAT

Downstream 100 bases:

>100_bases
TTTGGAAAATTGGCTACGTTGTTTTGGTAAGTCTTCGGATGCTCACGTATTAAGTATATGCTCCGCTCCTCGGCTTACAG
ACTCCTGGCTCTTTCCAAAT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 835; Mature: 835

Protein sequence:

>835_residues
MNQIEQKWQYIWQEEKAFEVSNASSKPKYYVLEMLPYPSGKIHVGHVRNYSIGDVIARFMTMQGFNVLHPMGWDAFGLPA
EHAAIKNNSHPKKWTYSNIKNMKKQLKSMGFSYDWSREINSCDPEYYKHEQKFFLELYERNLAYQKESFVNWDPVDNTVL
ANEQVVDGRGWRSGAIVVKRYLKQWFLKITDYAEELLNEIQNLKEWPEAVRSMQEKWIGKSIGANFHFKIKDNEETTIEV
FSTKPETIFGASFIGIAFNHPIIERLVSKTPEILAFITQCSHITRSSELEKTEREGVFTGLFVIHPFDSNIVLPVIITNF
VLMDYGTGAIFGCPAHDECDHELAVKMNLSIKQVIKADMDVQKTAYTEDGILINSDFLNGLTSHEAKQEVIGEFEKLGIG
KRSVNYRLKDWGISRQRFWGCPIPMIHCEICGIVPVPYKDLPVILPDDVNFDGHGNPLDHHPSWKHVNCPKCDKPAVRET
DTFDTFFESSWYFMRYCNSNATEMTDKKACDYWLPVDKYIGGIEHAVMHLLYARFFTKVMNEQNYVSVREPFKGLFTQGM
VLHATYKDEHNNWLYPEEVVKKGNEFFHKESNNRVVQGRIEKMSKSKKNLIDLETMQEQYGADAIRLFVLSDSPPEKDLE
WSASGIEGCSRFINKLEYMFKAIDSLKDDVNSEVNKELNRLVHFTIKHVAEDIKHFALNRAIARMRELSNAISAEISKDK
IDVKTVRHGFNVLVQLLNPFIPHITEEIWQKLGNKERLYNLSFPAFDESMLELDTYIMAVQVNGKLRDTYEFKTSVSEDE
IKQVTVSLPKVQKFLEGQEPKKIILVPRKIVNILV

Sequences:

>Translated_835_residues
MNQIEQKWQYIWQEEKAFEVSNASSKPKYYVLEMLPYPSGKIHVGHVRNYSIGDVIARFMTMQGFNVLHPMGWDAFGLPA
EHAAIKNNSHPKKWTYSNIKNMKKQLKSMGFSYDWSREINSCDPEYYKHEQKFFLELYERNLAYQKESFVNWDPVDNTVL
ANEQVVDGRGWRSGAIVVKRYLKQWFLKITDYAEELLNEIQNLKEWPEAVRSMQEKWIGKSIGANFHFKIKDNEETTIEV
FSTKPETIFGASFIGIAFNHPIIERLVSKTPEILAFITQCSHITRSSELEKTEREGVFTGLFVIHPFDSNIVLPVIITNF
VLMDYGTGAIFGCPAHDECDHELAVKMNLSIKQVIKADMDVQKTAYTEDGILINSDFLNGLTSHEAKQEVIGEFEKLGIG
KRSVNYRLKDWGISRQRFWGCPIPMIHCEICGIVPVPYKDLPVILPDDVNFDGHGNPLDHHPSWKHVNCPKCDKPAVRET
DTFDTFFESSWYFMRYCNSNATEMTDKKACDYWLPVDKYIGGIEHAVMHLLYARFFTKVMNEQNYVSVREPFKGLFTQGM
VLHATYKDEHNNWLYPEEVVKKGNEFFHKESNNRVVQGRIEKMSKSKKNLIDLETMQEQYGADAIRLFVLSDSPPEKDLE
WSASGIEGCSRFINKLEYMFKAIDSLKDDVNSEVNKELNRLVHFTIKHVAEDIKHFALNRAIARMRELSNAISAEISKDK
IDVKTVRHGFNVLVQLLNPFIPHITEEIWQKLGNKERLYNLSFPAFDESMLELDTYIMAVQVNGKLRDTYEFKTSVSEDE
IKQVTVSLPKVQKFLEGQEPKKIILVPRKIVNILV
>Mature_835_residues
MNQIEQKWQYIWQEEKAFEVSNASSKPKYYVLEMLPYPSGKIHVGHVRNYSIGDVIARFMTMQGFNVLHPMGWDAFGLPA
EHAAIKNNSHPKKWTYSNIKNMKKQLKSMGFSYDWSREINSCDPEYYKHEQKFFLELYERNLAYQKESFVNWDPVDNTVL
ANEQVVDGRGWRSGAIVVKRYLKQWFLKITDYAEELLNEIQNLKEWPEAVRSMQEKWIGKSIGANFHFKIKDNEETTIEV
FSTKPETIFGASFIGIAFNHPIIERLVSKTPEILAFITQCSHITRSSELEKTEREGVFTGLFVIHPFDSNIVLPVIITNF
VLMDYGTGAIFGCPAHDECDHELAVKMNLSIKQVIKADMDVQKTAYTEDGILINSDFLNGLTSHEAKQEVIGEFEKLGIG
KRSVNYRLKDWGISRQRFWGCPIPMIHCEICGIVPVPYKDLPVILPDDVNFDGHGNPLDHHPSWKHVNCPKCDKPAVRET
DTFDTFFESSWYFMRYCNSNATEMTDKKACDYWLPVDKYIGGIEHAVMHLLYARFFTKVMNEQNYVSVREPFKGLFTQGM
VLHATYKDEHNNWLYPEEVVKKGNEFFHKESNNRVVQGRIEKMSKSKKNLIDLETMQEQYGADAIRLFVLSDSPPEKDLE
WSASGIEGCSRFINKLEYMFKAIDSLKDDVNSEVNKELNRLVHFTIKHVAEDIKHFALNRAIARMRELSNAISAEISKDK
IDVKTVRHGFNVLVQLLNPFIPHITEEIWQKLGNKERLYNLSFPAFDESMLELDTYIMAVQVNGKLRDTYEFKTSVSEDE
IKQVTVSLPKVQKFLEGQEPKKIILVPRKIVNILV

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=874, Percent_Identity=37.070938215103, Blast_Score=555, Evalue=1e-158,
Organism=Homo sapiens, GI46852147, Length=875, Percent_Identity=20.4571428571429, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1786861, Length=855, Percent_Identity=45.0292397660819, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=411, Percent_Identity=21.4111922141119, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71997510, Length=779, Percent_Identity=30.0385109114249, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71997517, Length=779, Percent_Identity=30.0385109114249, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI212645227, Length=316, Percent_Identity=29.1139240506329, Blast_Score=153, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323414, Length=853, Percent_Identity=37.9835873388042, Blast_Score=553, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6321531, Length=418, Percent_Identity=22.2488038277512, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325217, Length=846, Percent_Identity=20.5673758865248, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355409, Length=778, Percent_Identity=33.9331619537275, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI17864482, Length=432, Percent_Identity=22.9166666666667, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24653289, Length=432, Percent_Identity=22.9166666666667, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281366294, Length=167, Percent_Identity=26.9461077844311, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_RICAE (C3PNE7)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845200.1
- ProteinModelPortal:   C3PNE7
- GeneID:   7814963
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0546
- OMA:   YARYCSP
- ProtClustDB:   PRK00390
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 96748; Mature: 96748

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 605-605

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIEQKWQYIWQEEKAFEVSNASSKPKYYVLEMLPYPSGKIHVGHVRNYSIGDVIARFM
CCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHH
TMQGFNVLHPMGWDAFGLPAEHAAIKNNSHPKKWTYSNIKNMKKQLKSMGFSYDWSREIN
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
SCDPEYYKHEQKFFLELYERNLAYQKESFVNWDPVDNTVLANEQVVDGRGWRSGAIVVKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCEEECCCCCCCHHHHHHH
YLKQWFLKITDYAEELLNEIQNLKEWPEAVRSMQEKWIGKSIGANFHFKIKDNEETTIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
FSTKPETIFGASFIGIAFNHPIIERLVSKTPEILAFITQCSHITRSSELEKTEREGVFTG
EECCCCEEECCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LFVIHPFDSNIVLPVIITNFVLMDYGTGAIFGCPAHDECDHELAVKMNLSIKQVIKADMD
EEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHCCC
VQKTAYTEDGILINSDFLNGLTSHEAKQEVIGEFEKLGIGKRSVNYRLKDWGISRQRFWG
HHHHEECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHCCC
CPIPMIHCEICGIVPVPYKDLPVILPDDVNFDGHGNPLDHHPSWKHVNCPKCDKPAVRET
CCCCCEEHHEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTFDTFFESSWYFMRYCNSNATEMTDKKACDYWLPVDKYIGGIEHAVMHLLYARFFTKVM
CCHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
NEQNYVSVREPFKGLFTQGMVLHATYKDEHNNWLYPEEVVKKGNEFFHKESNNRVVQGRI
CCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHCCCCCCEEHHHH
EKMSKSKKNLIDLETMQEQYGADAIRLFVLSDSPPEKDLEWSASGIEGCSRFINKLEYMF
HHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KAIDSLKDDVNSEVNKELNRLVHFTIKHVAEDIKHFALNRAIARMRELSNAISAEISKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IDVKTVRHGFNVLVQLLNPFIPHITEEIWQKLGNKERLYNLSFPAFDESMLELDTYIMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHEEEEEEE
QVNGKLRDTYEFKTSVSEDEIKQVTVSLPKVQKFLEGQEPKKIILVPRKIVNILV
EECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCC
>Mature Secondary Structure
MNQIEQKWQYIWQEEKAFEVSNASSKPKYYVLEMLPYPSGKIHVGHVRNYSIGDVIARFM
CCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHH
TMQGFNVLHPMGWDAFGLPAEHAAIKNNSHPKKWTYSNIKNMKKQLKSMGFSYDWSREIN
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
SCDPEYYKHEQKFFLELYERNLAYQKESFVNWDPVDNTVLANEQVVDGRGWRSGAIVVKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCEEECCCCCCCHHHHHHH
YLKQWFLKITDYAEELLNEIQNLKEWPEAVRSMQEKWIGKSIGANFHFKIKDNEETTIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
FSTKPETIFGASFIGIAFNHPIIERLVSKTPEILAFITQCSHITRSSELEKTEREGVFTG
EECCCCEEECCHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LFVIHPFDSNIVLPVIITNFVLMDYGTGAIFGCPAHDECDHELAVKMNLSIKQVIKADMD
EEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHCCC
VQKTAYTEDGILINSDFLNGLTSHEAKQEVIGEFEKLGIGKRSVNYRLKDWGISRQRFWG
HHHHEECCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHCCC
CPIPMIHCEICGIVPVPYKDLPVILPDDVNFDGHGNPLDHHPSWKHVNCPKCDKPAVRET
CCCCCEEHHEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTFDTFFESSWYFMRYCNSNATEMTDKKACDYWLPVDKYIGGIEHAVMHLLYARFFTKVM
CCHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
NEQNYVSVREPFKGLFTQGMVLHATYKDEHNNWLYPEEVVKKGNEFFHKESNNRVVQGRI
CCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHCCHHHHHCCCCCCEEHHHH
EKMSKSKKNLIDLETMQEQYGADAIRLFVLSDSPPEKDLEWSASGIEGCSRFINKLEYMF
HHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KAIDSLKDDVNSEVNKELNRLVHFTIKHVAEDIKHFALNRAIARMRELSNAISAEISKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
IDVKTVRHGFNVLVQLLNPFIPHITEEIWQKLGNKERLYNLSFPAFDESMLELDTYIMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHEEEEEEE
QVNGKLRDTYEFKTSVSEDEIKQVTVSLPKVQKFLEGQEPKKIILVPRKIVNILV
EECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA