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The map label for this gene is dapF

Identifier: 229586693

GI number: 229586693

Start: 566345

End: 567157

Strand: Direct

Name: dapF

Synonym: RAF_ORF0540

Alternate gene names: 229586693

Gene position: 566345-567157 (Clockwise)

Preceding gene: 229586688

Following gene: 229586694

Centisome position: 44.3

GC content: 34.56

Gene sequence:

>813_bases
ATGATTAGTAAAATTAATTTTGTAAAAATGCATGGTCTCGGTAATGATTTTGTTATTGTTAACAAACGAGATTTATCAAG
TTCATATGATTTATCGCAGTTAGCAAAAAATATGGCTGAGCGTCATACAGGTATTGGTTGCGATCAGTTTATTATTTATG
AAGAGCATAATGATTTTTATGAAATGATTATATATAACATAGACGGCTCTAGTGCTAAATTATGCGGTAATGCTACAAGA
TGTTTAGCCAAGTTAATTTATCTTGATACGGGAAAGCAAGATATTACTGTAATGGTAGGCAATAAAAAATTGCTATGTAA
TGTCAATGATGAAAATAATATTAGCGTTAATGTAGGAAGCGTAAGTTTTAATGAAGCTTGGATGCCAAATCGTGATAAAG
TTTGGGAATTTGCAGAGCGTTATATGATTGATTTAAAGGAAACTATTTGCGTTGATATAGGTAATCCGCATGTAGTTATT
TTTAGTAAGTTAGAACCTCAAGATCAAAAAATTGTCGGTGAAAGATTGCAGGCTAAGGAATTATTTGCAGATGGGGTAAA
CGTTAATTTTGCTGAAGTAAAAGATAATAAAATCTATTTATCCGTTTGGGAGCGGGGGGCAGGGTTAACCCTTGCTTGCG
GAAGCGGAGCTTGCGGTAGTTTTGCTGCCGGTTTAAAGCGCGGTTTTATTCATTCACCAAGTACTATAGTCTTTAAGCAC
GGTAACCTTACTATGAAAGAAGAAAACGGTAATATAATAATGCAAGGGGCAGCTACGCTTGTAGCACGTGGGGAATATTA
TTGTGAGCAATAA

Upstream 100 bases:

>100_bases
TGAAATTAGCATAATAGTCTATATTTTTATATATCATTACTTCATTATAGTAAATTCCAAGAAATTTGCTATAATACAAT
TTCCTAATATGTAAAACATT

Downstream 100 bases:

>100_bases
TTTAAGACAAAACGTAGTAACATTTGGCTGTAGACTTAATATTTACGAAAGTGAGATAATACGAAAAAACTTGGAATTGT
CGGGTATCGATAATGTAGCA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MISKINFVKMHGLGNDFVIVNKRDLSSSYDLSQLAKNMAERHTGIGCDQFIIYEEHNDFYEMIIYNIDGSSAKLCGNATR
CLAKLIYLDTGKQDITVMVGNKKLLCNVNDENNISVNVGSVSFNEAWMPNRDKVWEFAERYMIDLKETICVDIGNPHVVI
FSKLEPQDQKIVGERLQAKELFADGVNVNFAEVKDNKIYLSVWERGAGLTLACGSGACGSFAAGLKRGFIHSPSTIVFKH
GNLTMKEENGNIIMQGAATLVARGEYYCEQ

Sequences:

>Translated_270_residues
MISKINFVKMHGLGNDFVIVNKRDLSSSYDLSQLAKNMAERHTGIGCDQFIIYEEHNDFYEMIIYNIDGSSAKLCGNATR
CLAKLIYLDTGKQDITVMVGNKKLLCNVNDENNISVNVGSVSFNEAWMPNRDKVWEFAERYMIDLKETICVDIGNPHVVI
FSKLEPQDQKIVGERLQAKELFADGVNVNFAEVKDNKIYLSVWERGAGLTLACGSGACGSFAAGLKRGFIHSPSTIVFKH
GNLTMKEENGNIIMQGAATLVARGEYYCEQ
>Mature_270_residues
MISKINFVKMHGLGNDFVIVNKRDLSSSYDLSQLAKNMAERHTGIGCDQFIIYEEHNDFYEMIIYNIDGSSAKLCGNATR
CLAKLIYLDTGKQDITVMVGNKKLLCNVNDENNISVNVGSVSFNEAWMPNRDKVWEFAERYMIDLKETICVDIGNPHVVI
FSKLEPQDQKIVGERLQAKELFADGVNVNFAEVKDNKIYLSVWERGAGLTLACGSGACGSFAAGLKRGFIHSPSTIVFKH
GNLTMKEENGNIIMQGAATLVARGEYYCEQ

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=273, Percent_Identity=34.0659340659341, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_RICAE (C3PNE1)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845194.1
- GeneID:   7814957
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0540
- OMA:   DIGNPHL
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30086; Mature: 30086

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 213-213

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKINFVKMHGLGNDFVIVNKRDLSSSYDLSQLAKNMAERHTGIGCDQFIIYEEHNDFY
CCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
EMIIYNIDGSSAKLCGNATRCLAKLIYLDTGKQDITVMVGNKKLLCNVNDENNISVNVGS
EEEEEEECCCCCEECCHHHHHHHHHHEEECCCCEEEEEECCEEEEEEECCCCEEEEEECC
VSFNEAWMPNRDKVWEFAERYMIDLKETICVDIGNPHVVIFSKLEPQDQKIVGERLQAKE
EEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LFADGVNVNFAEVKDNKIYLSVWERGAGLTLACGSGACGSFAAGLKRGFIHSPSTIVFKH
HHHCCCCEEEEEEECCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEC
GNLTMKEENGNIIMQGAATLVARGEYYCEQ
CCEEEEECCCCEEEEECEEEEECCCEECCC
>Mature Secondary Structure
MISKINFVKMHGLGNDFVIVNKRDLSSSYDLSQLAKNMAERHTGIGCDQFIIYEEHNDFY
CCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEE
EMIIYNIDGSSAKLCGNATRCLAKLIYLDTGKQDITVMVGNKKLLCNVNDENNISVNVGS
EEEEEEECCCCCEECCHHHHHHHHHHEEECCCCEEEEEECCEEEEEEECCCCEEEEEECC
VSFNEAWMPNRDKVWEFAERYMIDLKETICVDIGNPHVVIFSKLEPQDQKIVGERLQAKE
EEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LFADGVNVNFAEVKDNKIYLSVWERGAGLTLACGSGACGSFAAGLKRGFIHSPSTIVFKH
HHHCCCCEEEEEEECCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEC
GNLTMKEENGNIIMQGAATLVARGEYYCEQ
CCEEEEECCCCEEEEECEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA