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The map label for this gene is lspA

Identifier: 229586683

GI number: 229586683

Start: 551606

End: 552211

Strand: Direct

Name: lspA

Synonym: RAF_ORF0520

Alternate gene names: 229586683

Gene position: 551606-552211 (Clockwise)

Preceding gene: 229586682

Following gene: 229586684

Centisome position: 43.14

GC content: 30.69

Gene sequence:

>606_bases
ATGCTCCCACTATTAAAAAAACTATATCTCACATTTGCTCGTAGTAGTCGTATAATAATAACCTTAGTGATTATTGATCA
GTTAAGTAAATGGTGGTTTATTGATAATTTGAGATGGAAGCCGGACTTAATGCTTAAGTTTACTTCTTTCTTAAATATGG
TTTATACTTGGAATTACGGTATTAGCTTTGGTCTAATGCGTGAATATTATCAATATAGCAATGCTATTTTCTTAATAACG
AACACGATTATTGTCTGTTATTTATATTATTTGATGATACGTTCAAAAACGATCGGAAGCTTTGCCGGTTATAGCTTTGT
AATCGGCGGAGCTGTTGGTAATCTAATTGATAGATTTTTTAGAGGAGCAGTTTTTGACTTCATCCATTTTCATTATCAGA
ATTATAGCTTCCCCGTATTTAATTTAGCTGATTGCTTTATTACAATAGGAGTAATTATCTTAATAGAAGATTATTATAGT
ACTAAAAAAGTTATTGAAGAAAAGGCTAAAGGGAATTATGATAATGCTCAAATTGAAGCTATGGCTGAAAAAATTCGTAA
TACCGATAAGGGTGGTAACGATAAAATAGCCTCCTTGCAAAATTAA

Upstream 100 bases:

>100_bases
TTGTTTTTTATAGGTTTATTTTATCAAATATATAATTTTGTATTAATATTAAGGTTATTTTTTCTGGATTCCCGTTTTTG
CGGGAATGACATTTACAATT

Downstream 100 bases:

>100_bases
ACTTATAGAGAGGAATTTGAAGGAAACACAGAACGCAGAACCGTAGCGTAGTAGACCTACGTGAGGATTCAAGTACCGGA
TTGACGTACAAATTACCTCT

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MLPLLKKLYLTFARSSRIIITLVIIDQLSKWWFIDNLRWKPDLMLKFTSFLNMVYTWNYGISFGLMREYYQYSNAIFLIT
NTIIVCYLYYLMIRSKTIGSFAGYSFVIGGAVGNLIDRFFRGAVFDFIHFHYQNYSFPVFNLADCFITIGVIILIEDYYS
TKKVIEEKAKGNYDNAQIEAMAEKIRNTDKGGNDKIASLQN

Sequences:

>Translated_201_residues
MLPLLKKLYLTFARSSRIIITLVIIDQLSKWWFIDNLRWKPDLMLKFTSFLNMVYTWNYGISFGLMREYYQYSNAIFLIT
NTIIVCYLYYLMIRSKTIGSFAGYSFVIGGAVGNLIDRFFRGAVFDFIHFHYQNYSFPVFNLADCFITIGVIILIEDYYS
TKKVIEEKAKGNYDNAQIEAMAEKIRNTDKGGNDKIASLQN
>Mature_201_residues
MLPLLKKLYLTFARSSRIIITLVIIDQLSKWWFIDNLRWKPDLMLKFTSFLNMVYTWNYGISFGLMREYYQYSNAIFLIT
NTIIVCYLYYLMIRSKTIGSFAGYSFVIGGAVGNLIDRFFRGAVFDFIHFHYQNYSFPVFNLADCFITIGVIILIEDYYS
TKKVIEEKAKGNYDNAQIEAMAEKIRNTDKGGNDKIASLQN

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=149, Percent_Identity=32.2147651006711, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_RICAE (C3PND1)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845184.1
- MEROPS:   A08.001
- GeneID:   7814944
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0520
- OMA:   ICSKTIG
- ProtClustDB:   PRK00376
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 23484; Mature: 23484

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 117-117 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

HASH(0x2106e394)-; HASH(0x2175af28)-; HASH(0x21a0c8d4)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLLKKLYLTFARSSRIIITLVIIDQLSKWWFIDNLRWKPDLMLKFTSFLNMVYTWNYG
CCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHEECCC
ISFGLMREYYQYSNAIFLITNTIIVCYLYYLMIRSKTIGSFAGYSFVIGGAVGNLIDRFF
CHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHH
RGAVFDFIHFHYQNYSFPVFNLADCFITIGVIILIEDYYSTKKVIEEKAKGNYDNAQIEA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MAEKIRNTDKGGNDKIASLQN
HHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure
MLPLLKKLYLTFARSSRIIITLVIIDQLSKWWFIDNLRWKPDLMLKFTSFLNMVYTWNYG
CCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHEECCC
ISFGLMREYYQYSNAIFLITNTIIVCYLYYLMIRSKTIGSFAGYSFVIGGAVGNLIDRFF
CHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHH
RGAVFDFIHFHYQNYSFPVFNLADCFITIGVIILIEDYYSTKKVIEEKAKGNYDNAQIEA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MAEKIRNTDKGGNDKIASLQN
HHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA