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The map label for this gene is hslU [H]

Identifier: 229586582

GI number: 229586582

Start: 430835

End: 432187

Strand: Direct

Name: hslU [H]

Synonym: RAF_ORF0403

Alternate gene names: 229586582

Gene position: 430835-432187 (Clockwise)

Preceding gene: 229586581

Following gene: 229586583

Centisome position: 33.7

GC content: 35.03

Gene sequence:

>1353_bases
ATGAAAGCTACTAAAACTACTTATAAAAAAGATCCTATGGGGCTTACCCCTTCTCAAATAGTGAATGAACTTAATAGATT
TATCGTAGGTCAAGAAAAGGCCAAAAAAGCTGTTGCTATTGCACTTAGAAATCGTTGTCGTCGTAAAAGAGTAGAAGGTA
ATTTACGTAATGAAATAGTACCGAAAAATATTTTAATGATTGGTTCAACCGGTGTCGGGAAAACGGAAATAGCAAGACGC
CTTGCAAAGCTGACTAATTCTCCTTTCTATAAAATTGAAGCAACTAAATTTACCGAAGTCGGATATGTAGGGCGTGATGT
AGAATCAATAATTCGTGATTTAGTCGAAATAGCCGTTAATACTGAAAAAACTTTAGCAAAAACGAAAGTAGATATTCATG
CCCGTGAAAAAGCGATCGAGAGAATATTAGATAGTTTAGTAGGTAAAACTTCTAGTAGTGAGACTAGAGAAAAGTTCAAA
GAAAAAATTTTAAACGGCGAACTTGATGATAAGGAAATTGAAATTAGCGTAGCTGATACTACACCTGTCGGCGGTGGAAG
TTTTGAAATACCGGGTATGCCGGGAGCATCTATGGGCGTTCTTAATCTTGGCGATATGATTGGACGAGCCCTTGGCAGTA
GTAAGACTAAAACAAAAAAAATGCTAGTTAAAGATGCTATGGCTATTATTATACCTGAAGAATCAGAAAAATTAATAGAC
CAAGAAAAAATTATTCAGCAAGCTATAAATTTAGCTGAAAATGACGGTATAGTTTTTATTGACGAAATTGATAAAATAGC
TTCAACTGGTAGTTCCGGAGCAAAAAATGCCGCAATAAGTAGAGAGGGAGTGCAAAGAGATTTGCTACCTTTGATAGAGG
GCACGACGGTTAATACTAAATATGGACCGGTTAAAACCGATCATATATTATTTATTGCTTCCGGTGCTTTTCATATTGCT
AAACCTTCTGATTTATTACCGGAGTTACAAGGAAGGTTACCGATTAGAGTAGAATTAAATTCGCTTACCAAAGATGATAT
GATTAAAATATTGCTTGAGCCTGAAACTAGTTTAATAAAACAATATTCGGCGTTAATAGGTACTGAAGACGTACGTCTTG
AATTTGCTGCTTCTGCTATTGAGAAGATAGCGGATTATGCGATCACCGTTAATTTAGAAGTCGAAGATATAGGGGCTAGA
AGGCTGCATACTATACTTGAGAATTTGCTTGAGGATATAAGCTTTGAAGCTAGCGAAATGAAAGGCAAAAAAATAACTAT
CGATGATAAATTTGTAGAAAATCAATTATCAAAAATAATAACTAATCTCGACTTAGCTAAGTTTGTTCTATAG

Upstream 100 bases:

>100_bases
AGAATAATTTAACTGCTGAAGAAATTGCTTTAAAATCTATGAATATAGCTGCAGATTTGTGTGTATTTTCTAATCATAAT
ATTATAATGGAAAAAGTTGT

Downstream 100 bases:

>100_bases
TACTTTTTTCAATCAATGATTAAGGTAAGATTGTTATTTTTCATCATTACGAGGCAATTACGTCAGTAATTGACGAAGTA
ATCTCAGGAGTTTGTTATTA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKATKTTYKKDPMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTNSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTKVDIHAREKAIERILDSLVGKTSSSETREKFK
EKILNGELDDKEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDMIGRALGSSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAAISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVRLEFAASAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGKKITIDDKFVENQLSKIITNLDLAKFVL

Sequences:

>Translated_450_residues
MKATKTTYKKDPMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTNSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTKVDIHAREKAIERILDSLVGKTSSSETREKFK
EKILNGELDDKEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDMIGRALGSSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAAISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVRLEFAASAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGKKITIDDKFVENQLSKIITNLDLAKFVL
>Mature_450_residues
MKATKTTYKKDPMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTNSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTKVDIHAREKAIERILDSLVGKTSSSETREKFK
EKILNGELDDKEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDMIGRALGSSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAAISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVRLEFAASAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGKKITIDDKFVENQLSKIITNLDLAKFVL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=54.586129753915, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786642, Length=255, Percent_Identity=32.156862745098, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648291, Length=241, Percent_Identity=30.7053941908714, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648289, Length=241, Percent_Identity=30.7053941908714, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49554; Mature: 49554

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATKTTYKKDPMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
PKNILMIGSTGVGKTEIARRLAKLTNSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVN
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECEEHHCCCCCCCHHHHHHHHHHHHHC
TEKTLAKTKVDIHAREKAIERILDSLVGKTSSSETREKFKEKILNGELDDKEIEISVADT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
TPVGGGSFEIPGMPGASMGVLNLGDMIGRALGSSKTKTKKMLVKDAMAIIIPEESEKLID
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCHHHHHH
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAAISREGVQRDLLPLIEGTTVNTK
HHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCHHCHHHHHHHHHHHHCCCEECCC
YGPVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIK
CCCCCCCEEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHH
QYSALIGTEDVRLEFAASAIEKIADYAITVNLEVEDIGARRLHTILENLLEDISFEASEM
HHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCHHHC
KGKKITIDDKFVENQLSKIITNLDLAKFVL
CCCEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKATKTTYKKDPMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
PKNILMIGSTGVGKTEIARRLAKLTNSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVN
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECEEHHCCCCCCCHHHHHHHHHHHHHC
TEKTLAKTKVDIHAREKAIERILDSLVGKTSSSETREKFKEKILNGELDDKEIEISVADT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
TPVGGGSFEIPGMPGASMGVLNLGDMIGRALGSSKTKTKKMLVKDAMAIIIPEESEKLID
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCHHHHHH
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAAISREGVQRDLLPLIEGTTVNTK
HHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCHHCHHHHHHHHHHHHCCCEECCC
YGPVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIK
CCCCCCCEEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHH
QYSALIGTEDVRLEFAASAIEKIADYAITVNLEVEDIGARRLHTILENLLEDISFEASEM
HHHHHHCCCHHHHHHHHHHHHHHHCEEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCHHHC
KGKKITIDDKFVENQLSKIITNLDLAKFVL
CCCEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]