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The map label for this gene is hisS

Identifier: 229586565

GI number: 229586565

Start: 413997

End: 415241

Strand: Direct

Name: hisS

Synonym: RAF_ORF0386

Alternate gene names: 229586565

Gene position: 413997-415241 (Clockwise)

Preceding gene: 229586564

Following gene: 229586566

Centisome position: 32.38

GC content: 33.41

Gene sequence:

>1245_bases
ATGACTGAAAAACTGCAACCACTCCGAGGAATGAAAGATCTCTTGCCGGATGATTATAAAGTTCACGACTATATAATTAA
CAAAGCAAGAGATGTTGGAGTATTGTACGGCTATAAACAAATGAGTACTCCTCTTGTGGAGTATACTAAAGTCTTTAACC
GTTCGATGGGTGAAAGCTCTGACGTAATCAGCAAAGAAATCTATAGTTTTTTAGATAAAAGCAATGATTTCGTAGCATTA
CGACCTGAATTTACAGCATGTATTATAAGAAGCCTCATCTCAAACAGATTACAACATAAATTACCATTAAAGTTTTTCTC
TACCGGTCCTGTTTTTCGTTATGATAGACCGCAAGCAGGGCGTCAAAGACAATTTCACCAACTAAATTATGAGTATATCG
GTGCTAAAGGAGCAATTACTGATGCTGATACTTTGAAGTTAGCTGTTGATATATTAAAAGCACTCGAAATAGAGCAGGAT
ACTACCTTAGAGCTTAACTCTCTTGGATGTAATGAATCAAGAAGCGTTTATCAGCAAAAATTAGTAGAATATCTAAATGA
TTTTAAAGATCAATTATCAGAAGAAAGCAAAATAAGGTTATCCAAAAATCCAATGCGGATACTTGATTCTAAAAGTGAGA
CTGATCAGAAAATAATAGCAAACGCACCGGTTTTATCTGAATATTACACTGATGAATCCAAAGAATATTTTGAGGAGTTA
ATACAGTATCTAGATATTTTAGGTATGAAATATAGCATAAATCCACGCTTGGTTAGAGGGCTTGATTATTATTGCCATAC
TGCTTTTGAATTCACTACAAAAAAGTTAGGGAGCCAATCTACTATTCTAGCTGGTGGACGTTATGACGGGCTTGCTAAAA
TCATGGGTAATAACGATGATGTGCCGGCTATCGGTTTTGCTGCCGGTATTGAGCGAATTGCTTTAATGCGGGAATATAAT
ATATCTGAAGTTAAGCCGGTATTTGTATTACCTATAGGCAAAAATAATATTTGCTATGCTTTAGAAATTGTAGATAAATT
GCGAACAGAAAATATTGCTATTATCATAGAATCTCTAGGTAAAATAGCTAAAAGAATGCAACGCATTTTTAATGAAAATG
CTCAATTTATTATTTTTATAGGTGATGAAGAGCAGGCGAATAATAACTTAAAAATTAAAGACTTAAAAAAAGCAGAAGAA
TATATAGTAGATTTTGCAAAAGCTCTTGAGCTATTGAAGAAATAA

Upstream 100 bases:

>100_bases
ATGAAGTAGTACAAAATTCCTGAGATTGCCAAGTCAAGGCTTTGCCTCTCCTCGCAATGACGAGTTAGTAGTATCCACGC
AACAAAACAAAATTAACCAA

Downstream 100 bases:

>100_bases
GATTTTTTATTGTTATACCGTGAACCTTCCATGGTATCCATCAAAACTAACTTTAAGTAATAAGCATTTATGACTCTTGC
CACAAGCTCGGCATTGCCCG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESSDVISKEIYSFLDKSNDFVAL
RPEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAGRQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQD
TTLELNSLGCNESRSVYQQKLVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEEL
IQYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDDVPAIGFAAGIERIALMREYN
ISEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLGKIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEE
YIVDFAKALELLKK

Sequences:

>Translated_414_residues
MTEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESSDVISKEIYSFLDKSNDFVAL
RPEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAGRQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQD
TTLELNSLGCNESRSVYQQKLVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEEL
IQYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDDVPAIGFAAGIERIALMREYN
ISEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLGKIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEE
YIVDFAKALELLKK
>Mature_413_residues
TEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESSDVISKEIYSFLDKSNDFVALR
PEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAGRQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQDT
TLELNSLGCNESRSVYQQKLVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEELI
QYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDDVPAIGFAAGIERIALMREYNI
SEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLGKIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEEY
IVDFAKALELLKK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=411, Percent_Identity=38.1995133819951, Blast_Score=307, Evalue=7e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_RICAE (C3PN13)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845066.1
- GeneID:   7815061
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0386
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47487; Mature: 47356

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESS
CCCCCCHHHCHHHHCCCCCCHHHHHHHHHCCCHHEECHHHHCCHHHHHHHHHHHCCCCCH
DVISKEIYSFLDKSNDFVALRPEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAG
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCC
RQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQDTTLELNSLGCNESRSVYQQK
HHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
LVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEEL
HHHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHH
IQYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDD
HHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCC
VPAIGFAAGIERIALMREYNISEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLG
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHCCCCHHHHHHHHH
KIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEEYIVDFAKALELLKK
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESS
CCCCCHHHCHHHHCCCCCCHHHHHHHHHCCCHHEECHHHHCCHHHHHHHHHHHCCCCCH
DVISKEIYSFLDKSNDFVALRPEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAG
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCCC
RQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQDTTLELNSLGCNESRSVYQQK
HHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
LVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEEL
HHHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHH
IQYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDD
HHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCC
VPAIGFAAGIERIALMREYNISEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLG
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHCCCCHHHHHHHHH
KIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEEYIVDFAKALELLKK
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA