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The map label for this gene is xth1 [H]

Identifier: 229586510

GI number: 229586510

Start: 350874

End: 351689

Strand: Direct

Name: xth1 [H]

Synonym: RAF_ORF0322

Alternate gene names: 229586510

Gene position: 350874-351689 (Clockwise)

Preceding gene: 229586509

Following gene: 229586511

Centisome position: 27.44

GC content: 33.33

Gene sequence:

>816_bases
ATGAAGATAGTGACTTGGAATATTAATTCATTGCGTCTACGGATCGATTTATTAAGAAAATTAGCATATGAACATAAGCC
GGATATTATTTTACTTCAAGAAACAAAGGTAGCTGACTCATTATTTCCTCTTGAAGTTATTAAAAATATAGGCTATGAAC
ATGTAATATATTCAGGGCAGAAATCATATAACGGTGTTGCTATTATTTCAAAATTTCCTTTGAATAATGTTTTTTCATTG
GAGTTATATAATAGTGATAAAAGGCATATAGCAGCTATAGTTAATGATATAGAAATACATAATTTTTATGTTCCTGCCGG
TGGTGATATACCTGATATCGAGGTAAATACAAAATTTAAGCATAAGCTCGAATATGTAAGGTTAATGCAGGAATGGTTAA
CGACTAACCGTACTAAAAACGATAAAATGATTATTGTTGGGGACTTGAACATTGCTCCGCATGAACATGATGTATGGTCC
AGTAAGCAGTTGCGAAATGTTATCAGCCATACCGATATTGAACGCTCGTTATTGGTAGAGCTACAAAATGCGTTAGGTTT
TATTGATAGTAGCAGACATTTCGTATCGCTTGACGAAAAATTTTATACTTGGTGGAGTTATAGAAATATAGATTGGAAAA
AATCTAACAGAGGTAGAAGACTTGATCATATTTGGGTTAGCGATAATTTAAAAGGTGCATTATTTTCTATGCATTTACTA
TCAGAAGCACGTGATTGGTCTTCACCTTCGGATCATGTACCATATTTTGTGACTTTTAATTTACAGATGTCATCCCGTGG
TCAAGCCACGGGATGA

Upstream 100 bases:

>100_bases
ACACGGAACGCAGCACCGCAGCATACACTTAGTACGTGAGGATGCGAGTACCGGATTGACATACAAATTACCTTTAGAAG
GAGGTTATACAACAAGTCTA

Downstream 100 bases:

>100_bases
CAATAGTCTTAAGGAGTAATTAGTGGATATTAAGTTAGGAAAATATAAGCCGACAAAGGAAGAATATATAAAAAGCTTTA
AAGATATGCTCCTACTGCGT

Product: Exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKIVTWNINSLRLRIDLLRKLAYEHKPDIILLQETKVADSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIEVNTKFKHKLEYVRLMQEWLTTNRTKNDKMIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNALGFIDSSRHFVSLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSDNLKGALFSMHLL
SEARDWSSPSDHVPYFVTFNLQMSSRGQATG

Sequences:

>Translated_271_residues
MKIVTWNINSLRLRIDLLRKLAYEHKPDIILLQETKVADSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIEVNTKFKHKLEYVRLMQEWLTTNRTKNDKMIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNALGFIDSSRHFVSLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSDNLKGALFSMHLL
SEARDWSSPSDHVPYFVTFNLQMSSRGQATG
>Mature_271_residues
MKIVTWNINSLRLRIDLLRKLAYEHKPDIILLQETKVADSLFPLEVIKNIGYEHVIYSGQKSYNGVAIISKFPLNNVFSL
ELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIEVNTKFKHKLEYVRLMQEWLTTNRTKNDKMIIVGDLNIAPHEHDVWS
SKQLRNVISHTDIERSLLVELQNALGFIDSSRHFVSLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSDNLKGALFSMHLL
SEARDWSSPSDHVPYFVTFNLQMSSRGQATG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=29.6577946768061, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=29.6577946768061, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=29.6577946768061, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=31.3868613138686, Blast_Score=129, Evalue=2e-31,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=30.1818181818182, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=30.1818181818182, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31610; Mature: 31610

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTWNINSLRLRIDLLRKLAYEHKPDIILLQETKVADSLFPLEVIKNIGYEHVIYSGQ
CEEEEEECCCEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCC
KSYNGVAIISKFPLNNVFSLELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIEVNTKFK
CCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEHEEEEEEEEEECCCCCCCCEEECCHHH
HKLEYVRLMQEWLTTNRTKNDKMIIVGDLNIAPHEHDVWSSKQLRNVISHTDIERSLLVE
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQNALGFIDSSRHFVSLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSDNLKGALFSMHLL
HHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
SEARDWSSPSDHVPYFVTFNLQMSSRGQATG
HHHHCCCCCCCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKIVTWNINSLRLRIDLLRKLAYEHKPDIILLQETKVADSLFPLEVIKNIGYEHVIYSGQ
CEEEEEECCCEEHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEECCC
KSYNGVAIISKFPLNNVFSLELYNSDKRHIAAIVNDIEIHNFYVPAGGDIPDIEVNTKFK
CCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEHEEEEEEEEEECCCCCCCCEEECCHHH
HKLEYVRLMQEWLTTNRTKNDKMIIVGDLNIAPHEHDVWSSKQLRNVISHTDIERSLLVE
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQNALGFIDSSRHFVSLDEKFYTWWSYRNIDWKKSNRGRRLDHIWVSDNLKGALFSMHLL
HHHHHCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
SEARDWSSPSDHVPYFVTFNLQMSSRGQATG
HHHHCCCCCCCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]