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The map label for this gene is pbpE [H]

Identifier: 229586509

GI number: 229586509

Start: 349546

End: 350727

Strand: Direct

Name: pbpE [H]

Synonym: RAF_ORF0321

Alternate gene names: 229586509

Gene position: 349546-350727 (Clockwise)

Preceding gene: 229586508

Following gene: 229586510

Centisome position: 27.34

GC content: 29.36

Gene sequence:

>1182_bases
ATGATGCGTAGATATTTTTTATTGTTGCTTTTTTTTATTACTAATTGTATTGATTGTTTTGCAGATATACAGCAAAGAAT
CAATGAAGCAGAAAAAGAATATATCAGTAATAGATTTTTAAATGCCGTTTTTATGTTTGCCGATGATTATAAACCTTTAT
TAACCGGTGCTAAAGGAATTTTTGCTTTAAATGGTGAGCAATTAAAAGCAAATGAAATGATGCCTATTGCTTCTGCTACA
AAGCCTTTTACAGCAGCGGGAATCTTAAAATTACAAGAACAGGCATTATTAAATATTAACGATAAAATTTATAAATATCT
TGATCCGGAAATGTGGGGTGGAAAAGTGCCGGATTGGGCATATAAAATATCTATCCATAATTTATTAACGCATAGTAGCG
GTATTGCTGAATATTTTAGTTTTGTTAAACTTGATTTAAATATGCCTAAGAAAGAGATTCACAAGAAAATATTACAATTT
GTTTCTTCTAAGCCTTTAGAAATACCTATAGGGAAAAAATTTAAATATAGTAATACTAATTTTGTTATACTCGGTATGAT
TATAGAAAAGGTGACTAAAAAAGATTTAGCTAACTTTTTTTATGATGAATTTTTTAAACCGCTTAATATGAAATCTACTA
GTTTTGCTTCATATAGTGAAGCCGCTAGAATCCAAAAAAACGTTATTAGTTCTAATTATCCGGTAAGATATTTTGTAACG
CCTAATAATAGTAATAAACCTATATTTACTCCTGTAACTGCTGATTTTTTAGCAGTTCCTTGTGCTGACGGTGGTATAAT
ATCAACACCGGTTGATCTTATTAGATGGTATAGGGCATTAAATGAGGAGAAAATTCTTTCTAAGAAGTCGTATAAACTTA
TGACTACTAAATATTTTTTAGCACAAGATATAGAAGGGCGTAAGTCTTATATGGGCTACGGTATTTTTCTTACTGATCTT
GATTCTAAGCGTTTAATGATTCATTATACGGGAAAGGCCCTTGGAATACAAAGTGAAGCAGGATATGTTGTGCCTGATAA
TCTTTATTTTGCTATACTCAGTAATACTATGATAAAAATTCCTGAAGAAGAAAAAGATAAAATTGACATGAAAAATCCTT
TAAATCAACTTGGAATAATTTATTTTAGAGATGCAATAATAGGGGCAGCTATAAAAAATTAA

Upstream 100 bases:

>100_bases
GTGTACGCTGTGGTTCTGCGTTCCGTGTCTCCTTCAAATTTCTTTTTATTAGCTGCGTTATGTAAGACGTCTATTGATAA
GTTTGTGGAGTGTTGCAGTT

Downstream 100 bases:

>100_bases
ATAGACTTGTTGTGTAACCTATCTTATAAAGAGGAATTTGAAAGAAACACGGAACGCAGCACCGCAGCATACACTTAGTA
CGTGAGGATGCGAGTACCGG

Product: Penicillin-binding protein 4

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MMRRYFLLLLFFITNCIDCFADIQQRINEAEKEYISNRFLNAVFMFADDYKPLLTGAKGIFALNGEQLKANEMMPIASAT
KPFTAAGILKLQEQALLNINDKIYKYLDPEMWGGKVPDWAYKISIHNLLTHSSGIAEYFSFVKLDLNMPKKEIHKKILQF
VSSKPLEIPIGKKFKYSNTNFVILGMIIEKVTKKDLANFFYDEFFKPLNMKSTSFASYSEAARIQKNVISSNYPVRYFVT
PNNSNKPIFTPVTADFLAVPCADGGIISTPVDLIRWYRALNEEKILSKKSYKLMTTKYFLAQDIEGRKSYMGYGIFLTDL
DSKRLMIHYTGKALGIQSEAGYVVPDNLYFAILSNTMIKIPEEEKDKIDMKNPLNQLGIIYFRDAIIGAAIKN

Sequences:

>Translated_393_residues
MMRRYFLLLLFFITNCIDCFADIQQRINEAEKEYISNRFLNAVFMFADDYKPLLTGAKGIFALNGEQLKANEMMPIASAT
KPFTAAGILKLQEQALLNINDKIYKYLDPEMWGGKVPDWAYKISIHNLLTHSSGIAEYFSFVKLDLNMPKKEIHKKILQF
VSSKPLEIPIGKKFKYSNTNFVILGMIIEKVTKKDLANFFYDEFFKPLNMKSTSFASYSEAARIQKNVISSNYPVRYFVT
PNNSNKPIFTPVTADFLAVPCADGGIISTPVDLIRWYRALNEEKILSKKSYKLMTTKYFLAQDIEGRKSYMGYGIFLTDL
DSKRLMIHYTGKALGIQSEAGYVVPDNLYFAILSNTMIKIPEEEKDKIDMKNPLNQLGIIYFRDAIIGAAIKN
>Mature_393_residues
MMRRYFLLLLFFITNCIDCFADIQQRINEAEKEYISNRFLNAVFMFADDYKPLLTGAKGIFALNGEQLKANEMMPIASAT
KPFTAAGILKLQEQALLNINDKIYKYLDPEMWGGKVPDWAYKISIHNLLTHSSGIAEYFSFVKLDLNMPKKEIHKKILQF
VSSKPLEIPIGKKFKYSNTNFVILGMIIEKVTKKDLANFFYDEFFKPLNMKSTSFASYSEAARIQKNVISSNYPVRYFVT
PNNSNKPIFTPVTADFLAVPCADGGIISTPVDLIRWYRALNEEKILSKKSYKLMTTKYFLAQDIEGRKSYMGYGIFLTDL
DSKRLMIHYTGKALGIQSEAGYVVPDNLYFAILSNTMIKIPEEEKDKIDMKNPLNQLGIIYFRDAIIGAAIKN

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1786574, Length=360, Percent_Identity=23.0555555555556, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533026, Length=338, Percent_Identity=27.810650887574, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 44976; Mature: 44976

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRYFLLLLFFITNCIDCFADIQQRINEAEKEYISNRFLNAVFMFADDYKPLLTGAKGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCEE
FALNGEQLKANEMMPIASATKPFTAAGILKLQEQALLNINDKIYKYLDPEMWGGKVPDWA
EEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCCCCCEE
YKISIHNLLTHSSGIAEYFSFVKLDLNMPKKEIHKKILQFVSSKPLEIPIGKKFKYSNTN
EEEEHHHHHHCCCCHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
FVILGMIIEKVTKKDLANFFYDEFFKPLNMKSTSFASYSEAARIQKNVISSNYPVRYFVT
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
PNNSNKPIFTPVTADFLAVPCADGGIISTPVDLIRWYRALNEEKILSKKSYKLMTTKYFL
CCCCCCCEEEECCCCCEEEECCCCCEECCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHH
AQDIEGRKSYMGYGIFLTDLDSKRLMIHYTGKALGIQSEAGYVVPDNLYFAILSNTMIKI
HHCCCCCHHHEEEEEEEEECCCCEEEEEECCCEEEEECCCCEECCCCEEEEEECCCEEEC
PEEEKDKIDMKNPLNQLGIIYFRDAIIGAAIKN
CCCCCCCCCCCCCHHHHCCCEEHHHHHHHHCCC
>Mature Secondary Structure
MMRRYFLLLLFFITNCIDCFADIQQRINEAEKEYISNRFLNAVFMFADDYKPLLTGAKGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHCCCCEE
FALNGEQLKANEMMPIASATKPFTAAGILKLQEQALLNINDKIYKYLDPEMWGGKVPDWA
EEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCCCCCEE
YKISIHNLLTHSSGIAEYFSFVKLDLNMPKKEIHKKILQFVSSKPLEIPIGKKFKYSNTN
EEEEHHHHHHCCCCHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
FVILGMIIEKVTKKDLANFFYDEFFKPLNMKSTSFASYSEAARIQKNVISSNYPVRYFVT
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
PNNSNKPIFTPVTADFLAVPCADGGIISTPVDLIRWYRALNEEKILSKKSYKLMTTKYFL
CCCCCCCEEEECCCCCEEEECCCCCEECCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHH
AQDIEGRKSYMGYGIFLTDLDSKRLMIHYTGKALGIQSEAGYVVPDNLYFAILSNTMIKI
HHCCCCCHHHEEEEEEEEECCCCEEEEEECCCEEEEECCCCEECCCCEEEEEECCCEEEC
PEEEKDKIDMKNPLNQLGIIYFRDAIIGAAIKN
CCCCCCCCCCCCCHHHHCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]