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The map label for this gene is barA [H]

Identifier: 229586477

GI number: 229586477

Start: 317195

End: 318688

Strand: Direct

Name: barA [H]

Synonym: RAF_ORF0289

Alternate gene names: 229586477

Gene position: 317195-318688 (Clockwise)

Preceding gene: 229586476

Following gene: 229586478

Centisome position: 24.81

GC content: 29.52

Gene sequence:

>1494_bases
ATGAACCATAATAAGTATATAGTAAGATTGTCATTCATTATATTGTTTCTTAATATTGTCATAAATATGATGTTTTATCG
TTATTTTATGATAAAAGAAATGATAGTAAAACAAGTGGCTTTGGAACATACTAAAATTGTAGAACTTTACACTGATAATA
TTTGGAATACTCATCAGAACGTAATCAGTAAATTACACAAATTCGATTATTTAAAACTATTACAAGATCAAGAGTTTATT
AATTTTGCAAAGATTACCGCTCAATGGTTCACCAATCTTAATATTAATATTTCTCTCTACGATCTCAAAGGTAATAAATT
CATCACTAGTAATATGTTACATATGTATAGTGTAGACAATTATAAAGATGATAGTTTGGTTGAGATAGTTACCGCAAAAA
TCGATAAATTTTTTTTTAAATCCTTTACTTCAAAAGCTCCGCTTCGAGATGCTTTTGAAGGTATTACAAGTCATATATTA
TTACCAAGGGTAGTAATAGAAAATGAAAGTGATTTAACCGTGGAAGAGGCTTCTTTTGTTACTAGCTATATCCCTGTTAT
AAGTAATAATTTAGATTTTCCCGTAGATGCAGTATTTGAGATTAACACTAATATTACCAGCCAGTGGCAAAATATAACCT
CTCTTGAGCAAAAGGTTTTTATAACTTTTATCATTATTTTTATAATATTCTGCACTATAATTATTAGTAATACTAACTAT
GCTAGACAAATCATTGAGGAGCAGCTTGAAACAAATAGAAATTTAAAAGCTCAAATAGTAAAAGTAGAAAAAACCAGCTC
TTCAAATACAAAGTTTTTTACTAATATTAGTCATGAATTGCGTACACCGCTTAATGCTATAATAGGCTTTTCAGAAATTC
TTATGTCGGAAAGAGATGCAGAAAAAAGTAAAAATTATATTAAAGATATAAATGATGCCGGTAAGCATTTGCTTAGTATG
ATTAATGATATTTTAGATCTTTCTAAAGCTTCTGCCGATAAATTAAAAGTAGATAGTATAGATCTTGATTTAAATAAATT
AATTCGTTCTTCGCTGATACTTGTTAAGACTCGTGCCGATCAAGCCGAAGTGGCTTTAATTAGTAGATTACCGAAAGAGC
ATGTGGTTATAAAGGCTGATCCAAAAAGGCTAAAACAAGTATTCTTAAATCTTTTATCAAATGCCGTGAAATTTACGAAT
TCCGGAGGAAGTGTTACTATTGCACTTGAAAAAGATGAGTTAGCAAAATTAGTATATATAAAAGTTATAGATACCGGTAT
CGGTATTGAGGAAAAAGATATCCCAAAAACTTTATCGGCGTTCGGTCAAATTGATAGTGAGCTTAGTAGAAAATATGAAG
GTACCGGTCTTGGGTTGCCGCTTACTAAAAAGCTGGTTGAACTTATGAACGGTAAATTTGATTTGCAAAGTAAGATAAAT
GAAGGTACCACCATAACAATAACGTTTACATATGACTGTAGTATTGAAATATAA

Upstream 100 bases:

>100_bases
CCACGCTCCTTTTAGTCACTCGCAATGACGATTTGGTATGCGAGCAAGCCTTCTGCAGGGATGACATCAGTTGAGTTAAA
AATAACTACTAGGTCTTATA

Downstream 100 bases:

>100_bases
AAAATTGTGTGGAATATTAAAAAAATTTCGGTATTATAAAAAAATTATAAATTTTTAAAAAATATATGAAACTGATTGTC
TTATTATTTACTTTTTTATT

Product: Histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNHNKYIVRLSFIILFLNIVINMMFYRYFMIKEMIVKQVALEHTKIVELYTDNIWNTHQNVISKLHKFDYLKLLQDQEFI
NFAKITAQWFTNLNINISLYDLKGNKFITSNMLHMYSVDNYKDDSLVEIVTAKIDKFFFKSFTSKAPLRDAFEGITSHIL
LPRVVIENESDLTVEEASFVTSYIPVISNNLDFPVDAVFEINTNITSQWQNITSLEQKVFITFIIIFIIFCTIIISNTNY
ARQIIEEQLETNRNLKAQIVKVEKTSSSNTKFFTNISHELRTPLNAIIGFSEILMSERDAEKSKNYIKDINDAGKHLLSM
INDILDLSKASADKLKVDSIDLDLNKLIRSSLILVKTRADQAEVALISRLPKEHVVIKADPKRLKQVFLNLLSNAVKFTN
SGGSVTIALEKDELAKLVYIKVIDTGIGIEEKDIPKTLSAFGQIDSELSRKYEGTGLGLPLTKKLVELMNGKFDLQSKIN
EGTTITITFTYDCSIEI

Sequences:

>Translated_497_residues
MNHNKYIVRLSFIILFLNIVINMMFYRYFMIKEMIVKQVALEHTKIVELYTDNIWNTHQNVISKLHKFDYLKLLQDQEFI
NFAKITAQWFTNLNINISLYDLKGNKFITSNMLHMYSVDNYKDDSLVEIVTAKIDKFFFKSFTSKAPLRDAFEGITSHIL
LPRVVIENESDLTVEEASFVTSYIPVISNNLDFPVDAVFEINTNITSQWQNITSLEQKVFITFIIIFIIFCTIIISNTNY
ARQIIEEQLETNRNLKAQIVKVEKTSSSNTKFFTNISHELRTPLNAIIGFSEILMSERDAEKSKNYIKDINDAGKHLLSM
INDILDLSKASADKLKVDSIDLDLNKLIRSSLILVKTRADQAEVALISRLPKEHVVIKADPKRLKQVFLNLLSNAVKFTN
SGGSVTIALEKDELAKLVYIKVIDTGIGIEEKDIPKTLSAFGQIDSELSRKYEGTGLGLPLTKKLVELMNGKFDLQSKIN
EGTTITITFTYDCSIEI
>Mature_497_residues
MNHNKYIVRLSFIILFLNIVINMMFYRYFMIKEMIVKQVALEHTKIVELYTDNIWNTHQNVISKLHKFDYLKLLQDQEFI
NFAKITAQWFTNLNINISLYDLKGNKFITSNMLHMYSVDNYKDDSLVEIVTAKIDKFFFKSFTSKAPLRDAFEGITSHIL
LPRVVIENESDLTVEEASFVTSYIPVISNNLDFPVDAVFEINTNITSQWQNITSLEQKVFITFIIIFIIFCTIIISNTNY
ARQIIEEQLETNRNLKAQIVKVEKTSSSNTKFFTNISHELRTPLNAIIGFSEILMSERDAEKSKNYIKDINDAGKHLLSM
INDILDLSKASADKLKVDSIDLDLNKLIRSSLILVKTRADQAEVALISRLPKEHVVIKADPKRLKQVFLNLLSNAVKFTN
SGGSVTIALEKDELAKLVYIKVIDTGIGIEEKDIPKTLSAFGQIDSELSRKYEGTGLGLPLTKKLVELMNGKFDLQSKIN
EGTTITITFTYDCSIEI

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=33.8582677165354, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=36.7965367965368, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=30.7086614173228, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=30.5084745762712, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=27.8225806451613, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790436, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=26.2931034482759, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788393, Length=238, Percent_Identity=26.0504201680672, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=23.4782608695652, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790346, Length=213, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790861, Length=217, Percent_Identity=26.7281105990783, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790551, Length=216, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=23.6051502145923, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=215, Percent_Identity=24.1860465116279, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=224, Percent_Identity=25, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=146, Percent_Identity=37.6712328767123, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56851; Mature: 56851

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHNKYIVRLSFIILFLNIVINMMFYRYFMIKEMIVKQVALEHTKIVELYTDNIWNTHQN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCHHHH
VISKLHKFDYLKLLQDQEFINFAKITAQWFTNLNINISLYDLKGNKFITSNMLHMYSVDN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCEEEEECCC
YKDDSLVEIVTAKIDKFFFKSFTSKAPLRDAFEGITSHILLPRVVIENESDLTVEEASFV
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEECCCCCCEEHHHHHH
TSYIPVISNNLDFPVDAVFEINTNITSQWQNITSLEQKVFITFIIIFIIFCTIIISNTNY
HHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARQIIEEQLETNRNLKAQIVKVEKTSSSNTKFFTNISHELRTPLNAIIGFSEILMSERDA
HHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHCCH
EKSKNYIKDINDAGKHLLSMINDILDLSKASADKLKVDSIDLDLNKLIRSSLILVKTRAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCEEEEEECCC
QAEVALISRLPKEHVVIKADPKRLKQVFLNLLSNAVKFTNSGGSVTIALEKDELAKLVYI
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHHHEEEE
KVIDTGIGIEEKDIPKTLSAFGQIDSELSRKYEGTGLGLPLTKKLVELMNGKFDLQSKIN
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHCC
EGTTITITFTYDCSIEI
CCCEEEEEEEEEEEEEC
>Mature Secondary Structure
MNHNKYIVRLSFIILFLNIVINMMFYRYFMIKEMIVKQVALEHTKIVELYTDNIWNTHQN
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCHHHH
VISKLHKFDYLKLLQDQEFINFAKITAQWFTNLNINISLYDLKGNKFITSNMLHMYSVDN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCEEEEECCC
YKDDSLVEIVTAKIDKFFFKSFTSKAPLRDAFEGITSHILLPRVVIENESDLTVEEASFV
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEECCCCCCEEHHHHHH
TSYIPVISNNLDFPVDAVFEINTNITSQWQNITSLEQKVFITFIIIFIIFCTIIISNTNY
HHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARQIIEEQLETNRNLKAQIVKVEKTSSSNTKFFTNISHELRTPLNAIIGFSEILMSERDA
HHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCCHHHHCHHHHHHHHHHHHHHHCCH
EKSKNYIKDINDAGKHLLSMINDILDLSKASADKLKVDSIDLDLNKLIRSSLILVKTRAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCEEEEEECCC
QAEVALISRLPKEHVVIKADPKRLKQVFLNLLSNAVKFTNSGGSVTIALEKDELAKLVYI
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHHHEEEE
KVIDTGIGIEEKDIPKTLSAFGQIDSELSRKYEGTGLGLPLTKKLVELMNGKFDLQSKIN
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHCC
EGTTITITFTYDCSIEI
CCCEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]