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The map label for this gene is rsmE [H]

Identifier: 229586410

GI number: 229586410

Start: 218917

End: 219657

Strand: Direct

Name: rsmE [H]

Synonym: RAF_ORF0201

Alternate gene names: 229586410

Gene position: 218917-219657 (Clockwise)

Preceding gene: 229586407

Following gene: 229586413

Centisome position: 17.12

GC content: 32.52

Gene sequence:

>741_bases
ATGAAATATAACCGTATTTATATTAATAGTCGTTTAGCTGAAAATAGCAAGATAGAATTAGCAAGTGATCATCATGTTCA
TTATGTCAAAACGGTGCTACGTCTTAAAGTAAATGATGGCTTACGTCTCTTTAACGGTACGGATGGAGAATTTCTAGCAC
AAATTAACGATATAGGTAAAAATAACCTATCGGTTAGACTTAAAGAACAGTTAAAAAAGCCTTATACAGAATCTACGTTA
ACTCTTGCTGTTGCTATAATAAAGCAAGATAAATTAATGCTTGCAATAAATATGGCTACGCAACTCGGTATAACTAAAAT
TATTCCGTTAATTACTAGGTGGTGTCAATTTAGGTCGGTAAATATCGAACGTTTAACAAAATGTGTTATTGAGGCAACAG
AGCAATCAGAACGTCTTACTCCCCCGATAATTGAAAAAGCTATCACAATACAAGATTACCTAAAAAAGAATAATAATTTG
ATGTTATATGCTAATGAACATGAAAAAGAAGAAAATTCTATATTACGAATTTCATCATCACTTAGTAATAGTGATATAAC
TATTATTGTAGGACCGGAGGGAGGCTTTACTAATGATGAGCTGGAACTTCTTGCCTCATATAAGAATACAAAATCTATAA
GTTTAGGAAGTAATATATTACGTGCTGAAACAGCAGCAATAACTGCTATAGCACAAGTGAGGTTGTTAGGAGTCATTGCG
AGGAAATTGCATAGCAATTGA

Upstream 100 bases:

>100_bases
ACGCTGTAGTGCTGCGTTCCGTGTCTCCTGTACATTCCTCTCTATAAGCTGGTTTGGAAAGATGTCTATTATCTTTTTTA
TCGTCCATAGTACTAAATAT

Downstream 100 bases:

>100_bases
CGAAGCAATCTCAGGAGTTTGTTATGATTTCATGAGATTGCCACGCAGCCTACGGCTGCTCGCAATGACGAAAAGGTAGA
GTTACTTCGCCTTTTCTATT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKYNRIYINSRLAENSKIELASDHHVHYVKTVLRLKVNDGLRLFNGTDGEFLAQINDIGKNNLSVRLKEQLKKPYTESTL
TLAVAIIKQDKLMLAINMATQLGITKIIPLITRWCQFRSVNIERLTKCVIEATEQSERLTPPIIEKAITIQDYLKKNNNL
MLYANEHEKEENSILRISSSLSNSDITIIVGPEGGFTNDELELLASYKNTKSISLGSNILRAETAAITAIAQVRLLGVIA
RKLHSN

Sequences:

>Translated_246_residues
MKYNRIYINSRLAENSKIELASDHHVHYVKTVLRLKVNDGLRLFNGTDGEFLAQINDIGKNNLSVRLKEQLKKPYTESTL
TLAVAIIKQDKLMLAINMATQLGITKIIPLITRWCQFRSVNIERLTKCVIEATEQSERLTPPIIEKAITIQDYLKKNNNL
MLYANEHEKEENSILRISSSLSNSDITIIVGPEGGFTNDELELLASYKNTKSISLGSNILRAETAAITAIAQVRLLGVIA
RKLHSN
>Mature_246_residues
MKYNRIYINSRLAENSKIELASDHHVHYVKTVLRLKVNDGLRLFNGTDGEFLAQINDIGKNNLSVRLKEQLKKPYTESTL
TLAVAIIKQDKLMLAINMATQLGITKIIPLITRWCQFRSVNIERLTKCVIEATEQSERLTPPIIEKAITIQDYLKKNNNL
MLYANEHEKEENSILRISSSLSNSDITIIVGPEGGFTNDELELLASYKNTKSISLGSNILRAETAAITAIAQVRLLGVIA
RKLHSN

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=245, Percent_Identity=31.0204081632653, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27688; Mature: 27688

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYNRIYINSRLAENSKIELASDHHVHYVKTVLRLKVNDGLRLFNGTDGEFLAQINDIGK
CCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCHHHHHHHHCCC
NNLSVRLKEQLKKPYTESTLTLAVAIIKQDKLMLAINMATQLGITKIIPLITRWCQFRSV
CCCEEEEHHHHCCCCCCHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NIERLTKCVIEATEQSERLTPPIIEKAITIQDYLKKNNNLMLYANEHEKEENSILRISSS
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECC
LSNSDITIIVGPEGGFTNDELELLASYKNTKSISLGSNILRAETAAITAIAQVRLLGVIA
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RKLHSN
HHHHCC
>Mature Secondary Structure
MKYNRIYINSRLAENSKIELASDHHVHYVKTVLRLKVNDGLRLFNGTDGEFLAQINDIGK
CCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCHHHHHHHHCCC
NNLSVRLKEQLKKPYTESTLTLAVAIIKQDKLMLAINMATQLGITKIIPLITRWCQFRSV
CCCEEEEHHHHCCCCCCHHHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NIERLTKCVIEATEQSERLTPPIIEKAITIQDYLKKNNNLMLYANEHEKEENSILRISSS
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECC
LSNSDITIIVGPEGGFTNDELELLASYKNTKSISLGSNILRAETAAITAIAQVRLLGVIA
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RKLHSN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]