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The map label for this gene is lasT [H]

Identifier: 229586358

GI number: 229586358

Start: 160370

End: 161062

Strand: Direct

Name: lasT [H]

Synonym: RAF_ORF0149

Alternate gene names: 229586358

Gene position: 160370-161062 (Clockwise)

Preceding gene: 229586357

Following gene: 229586362

Centisome position: 12.54

GC content: 34.78

Gene sequence:

>693_bases
ATGAATCCAATAATAATATTAGTTGCCCCGCAAATGGGTGCGAATATCGGTGCTACTGCAAGAGCAATGAAGAATTTTGG
CTTAAATGAGCTTAGAATCGTGGCTCCAAGAGACGGTTGGCCTAATGAGCAAGCACGTAGTAATGCAGTCGGAGCGGTTA
ATATTATAGATCATGCAAAAATTTTTGATAGCCTAGAGGATAGCATTAAAGACCTCGAATATTTATATGCTACTACTTGC
ATTAAAAGGGCTATGAATAAAGATTATGTATTTTCACAAAATTTACCTTTAGATTATCCAAATTCTGAAAAAGTCGGGAT
AATGTTCGGACGGGAAAATAATGGGCTTAGCAACGAAGAAATCGCATTTGCTAATAAGATTATCACTATTAATACAACCG
AATTTAGCTCATTAAACATTGCTCAAGCGGTTATTATTATATGCTATGAGTTGTTGCGTAATTCTACGCCCAGAGAGGAT
GTACATAACATTCAAAAATTAGCTACTAAAGAAGAAATAGAGCATTTTTTAGTAAATTTATTCGGCAAACTCGATAAAGC
TGGATTCTTCAAGGCTCCCGACAAAAAAACTGCTATGCAACAAAATATCACTAATATATTTACACGTATAAATAGTCTCT
CTTCCCCTGAAGTCCAAACTCTGCAAGGTATTATTAAATCTTTAAGTCAATGA

Upstream 100 bases:

>100_bases
ATAAAGCTTTTGCTGTCTGGCACTGCCTTAATTACCAATTCTGATGAAGCTGACGCCGTAGCGATTGCCTATACCTGCTT
AGTTACAAAAAATTATTAAA

Downstream 100 bases:

>100_bases
TATTTAGTAATTTCTTAAGTTCAGGAACATAATTTCTTATTCGGTTATATATTACATCAGCAAGCTTTTTATCGTAAGTA
TAAGATATCATATTACTACG

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNPIIILVAPQMGANIGATARAMKNFGLNELRIVAPRDGWPNEQARSNAVGAVNIIDHAKIFDSLEDSIKDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNEEIAFANKIITINTTEFSSLNIAQAVIIICYELLRNSTPRED
VHNIQKLATKEEIEHFLVNLFGKLDKAGFFKAPDKKTAMQQNITNIFTRINSLSSPEVQTLQGIIKSLSQ

Sequences:

>Translated_230_residues
MNPIIILVAPQMGANIGATARAMKNFGLNELRIVAPRDGWPNEQARSNAVGAVNIIDHAKIFDSLEDSIKDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNEEIAFANKIITINTTEFSSLNIAQAVIIICYELLRNSTPRED
VHNIQKLATKEEIEHFLVNLFGKLDKAGFFKAPDKKTAMQQNITNIFTRINSLSSPEVQTLQGIIKSLSQ
>Mature_230_residues
MNPIIILVAPQMGANIGATARAMKNFGLNELRIVAPRDGWPNEQARSNAVGAVNIIDHAKIFDSLEDSIKDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNEEIAFANKIITINTTEFSSLNIAQAVIIICYELLRNSTPRED
VHNIQKLATKEEIEHFLVNLFGKLDKAGFFKAPDKKTAMQQNITNIFTRINSLSSPEVQTLQGIIKSLSQ

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=178, Percent_Identity=37.6404494382022, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788881, Length=235, Percent_Identity=30.6382978723404, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.-

Molecular weight: Translated: 25691; Mature: 25691

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIIILVAPQMGANIGATARAMKNFGLNELRIVAPRDGWPNEQARSNAVGAVNIIDHAK
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEHHHHHHH
IFDSLEDSIKDLEYLYATTCIKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
IAFANKIITINTTEFSSLNIAQAVIIICYELLRNSTPREDVHNIQKLATKEEIEHFLVNL
EEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGKLDKAGFFKAPDKKTAMQQNITNIFTRINSLSSPEVQTLQGIIKSLSQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPIIILVAPQMGANIGATARAMKNFGLNELRIVAPRDGWPNEQARSNAVGAVNIIDHAK
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEHHHHHHH
IFDSLEDSIKDLEYLYATTCIKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
IAFANKIITINTTEFSSLNIAQAVIIICYELLRNSTPREDVHNIQKLATKEEIEHFLVNL
EEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGKLDKAGFFKAPDKKTAMQQNITNIFTRINSLSSPEVQTLQGIIKSLSQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]