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The map label for this gene is ruvC

Identifier: 229586357

GI number: 229586357

Start: 159895

End: 160368

Strand: Direct

Name: ruvC

Synonym: RAF_ORF0148

Alternate gene names: 229586357

Gene position: 159895-160368 (Clockwise)

Preceding gene: 229586356

Following gene: 229586358

Centisome position: 12.51

GC content: 36.92

Gene sequence:

>474_bases
ATGATAATACTTGGGATTGATCCGGCACTTGGGAGTCTTGGATGGGCAGTAGTGGCAAAGGAGAACGCACAGTTGAAATA
TTTAGCTAGTGGTATTATTAGAACAAATAGTAAGGATGCAATTCATCATAGACTTGCTTTTATTAATAGTACATTAGAAA
AAGTAATATTAGAATATCAACCAAATATGGCAGCGATTGAAGAAACATTTGTTAATACTAATAGCGTGACTTCTTTAAAG
CTTGGATATGCTAGAGGGGCGATAATGTCGCTGATCGGTAGATATAATTTGGACATGCGAGAGTTTAAGCCAAATACGGT
AAAAAAAACCGTAACAGGATATGGGCATGCAGAGAAAGACCAAATGTTGCATATGATAAAGCTTTTGCTGTCTGGCACTG
CCTTAATTACCAATTCTGATGAAGCTGACGCCGTAGCGATTGCCTATACCTGCTTAGTTACAAAAAATTATTAA

Upstream 100 bases:

>100_bases
CTTGACAGGCTTTGTTGGATCGTTTTCGATGTCATTCCCCGAAAGCGGGAATGACATAAAAGCCATATAATAATACCAAT
AAAAATAACATTAAATAATC

Downstream 100 bases:

>100_bases
AATGAATCCAATAATAATATTAGTTGCCCCGCAAATGGGTGCGAATATCGGTGCTACTGCAAGAGCAATGAAGAATTTTG
GCTTAAATGAGCTTAGAATC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MIILGIDPALGSLGWAVVAKENAQLKYLASGIIRTNSKDAIHHRLAFINSTLEKVILEYQPNMAAIEETFVNTNSVTSLK
LGYARGAIMSLIGRYNLDMREFKPNTVKKTVTGYGHAEKDQMLHMIKLLLSGTALITNSDEADAVAIAYTCLVTKNY

Sequences:

>Translated_157_residues
MIILGIDPALGSLGWAVVAKENAQLKYLASGIIRTNSKDAIHHRLAFINSTLEKVILEYQPNMAAIEETFVNTNSVTSLK
LGYARGAIMSLIGRYNLDMREFKPNTVKKTVTGYGHAEKDQMLHMIKLLLSGTALITNSDEADAVAIAYTCLVTKNY
>Mature_157_residues
MIILGIDPALGSLGWAVVAKENAQLKYLASGIIRTNSKDAIHHRLAFINSTLEKVILEYQPNMAAIEETFVNTNSVTSLK
LGYARGAIMSLIGRYNLDMREFKPNTVKKTVTGYGHAEKDQMLHMIKLLLSGTALITNSDEADAVAIAYTCLVTKNY

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=129, Percent_Identity=41.0852713178295, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_RICAE (C3PMF5)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844858.1
- GeneID:   7815397
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0148
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17247; Mature: 17247

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGIDPALGSLGWAVVAKENAQLKYLASGIIRTNSKDAIHHRLAFINSTLEKVILEYQ
CEEEECCCCHHCCCEEEEECCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PNMAAIEETFVNTNSVTSLKLGYARGAIMSLIGRYNLDMREFKPNTVKKTVTGYGHAEKD
CCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCCCCCHH
QMLHMIKLLLSGTALITNSDEADAVAIAYTCLVTKNY
HHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEECCC
>Mature Secondary Structure
MIILGIDPALGSLGWAVVAKENAQLKYLASGIIRTNSKDAIHHRLAFINSTLEKVILEYQ
CEEEECCCCHHCCCEEEEECCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PNMAAIEETFVNTNSVTSLKLGYARGAIMSLIGRYNLDMREFKPNTVKKTVTGYGHAEKD
CCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCCCCCHH
QMLHMIKLLLSGTALITNSDEADAVAIAYTCLVTKNY
HHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA