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The map label for this gene is mscM [H]

Identifier: 229586284

GI number: 229586284

Start: 66057

End: 67220

Strand: Direct

Name: mscM [H]

Synonym: RAF_ORF0067

Alternate gene names: 229586284

Gene position: 66057-67220 (Clockwise)

Preceding gene: 229586281

Following gene: 229586285

Centisome position: 5.17

GC content: 28.18

Gene sequence:

>1164_bases
ATGCAATATTTAATGGATCTATATCATACCTCAAGTACTGAGTTAGTATTATTGTTTGTTATGTTAGTATCTCTCATACC
GGTGATATTTCTAATTAAAAGATTAATTTTTATTCCGGTAAAAAATTATTTAACTAGACATCATTACGATGATTATGAAA
GGATAAAAAAATATCCTATATTTCGTTATTTATTACATACTTTATTAGCACTTTACTTTGTATGTTGGGGAAAGATTTTT
CATCCTACTTCTTTTAAAGCACATGTATTACTGGGAATCAAAGATACTATAGTAATCTTATATACTGGTATACCTGTGAC
GATGTTATTATTAACGCTTATAGATGCTTTTGCAGATCTGTATCACAATAGAATCAAAGCCGTTACAAAAAATGCACCAC
TTAGCTTATATTTTCAAATATTAAAAATTATTGTCATAGTTATTGCAGCAATGGTAACTATTTCATATATATTAAATATT
TCGCTTAGTACATTCTTAACAAGCTTAGGTGCAGCCACAGCTCTTTTAACATTTGTTTTCAAAGATACCGTTTTAGGATT
ACTTGCAAGCTTGCAACTAACTACTCAAGAAATTATTAATATTGGAGACTGGGTACGTATAGGCGCAATTGAAGGAACAG
TTGAAAAAATTACTATTTCCGTAGTAACAATTAGGAATTTTGACCATTCTATTTCCACAGTTCCTACTTACAGCATTTTA
AATTCTAACGTAACTAATTATAGAGGAATTAGTGAATCAGGAGCAAGAAGGGTAAAGAGAGTATTTAATATTAATATGGC
AACTATTAACTTCTGTGATGCTACTATTTTAAAAGCACTAAAAAAATCTCCTTATATATCAAAGGATGTAATAGATAAAA
TCACTCTTGATAAAGAAGAGAAAGATTTAACCAACATTAAACTATTCAAATTATATATTCAGGAATATCTAAAAAATAAT
CCAGCAGTTTATACCGAAGGGTTTACTTTTCTAGTAAGACAGCTTGAACCTACGATTAATGGGTTGCCTATAGAAATATA
TATCTTTGTTAAAGAAGTAAATTTAGTAGGTTATGAAAATATACAGGACAATATTTCTGAACATTTCATTTCTATACTTC
CTGAATTTAAATTAAAAATATTTCAGAATGTAGGAGTGGTATAA

Upstream 100 bases:

>100_bases
AGCAATACCAAATTCTTTTGAGTTAAAATTATTGATAAAAATAAAATTCTTAATAATAATAAAAAAGATTTATATTTACA
ATAATAATGATAGGTAAAAT

Downstream 100 bases:

>100_bases
TATATCCGAAGTAAAATGAAAAGTAGTAAAAATAAATTAAATTTGTATTCAAGATGGAAGTTGTCTTTCAGCTTGGATAT
TTACTACTTTTGAAAGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQYLMDLYHTSSTELVLLFVMLVSLIPVIFLIKRLIFIPVKNYLTRHHYDDYERIKKYPIFRYLLHTLLALYFVCWGKIF
HPTSFKAHVLLGIKDTIVILYTGIPVTMLLLTLIDAFADLYHNRIKAVTKNAPLSLYFQILKIIVIVIAAMVTISYILNI
SLSTFLTSLGAATALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGAIEGTVEKITISVVTIRNFDHSISTVPTYSIL
NSNVTNYRGISESGARRVKRVFNINMATINFCDATILKALKKSPYISKDVIDKITLDKEEKDLTNIKLFKLYIQEYLKNN
PAVYTEGFTFLVRQLEPTINGLPIEIYIFVKEVNLVGYENIQDNISEHFISILPEFKLKIFQNVGVV

Sequences:

>Translated_387_residues
MQYLMDLYHTSSTELVLLFVMLVSLIPVIFLIKRLIFIPVKNYLTRHHYDDYERIKKYPIFRYLLHTLLALYFVCWGKIF
HPTSFKAHVLLGIKDTIVILYTGIPVTMLLLTLIDAFADLYHNRIKAVTKNAPLSLYFQILKIIVIVIAAMVTISYILNI
SLSTFLTSLGAATALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGAIEGTVEKITISVVTIRNFDHSISTVPTYSIL
NSNVTNYRGISESGARRVKRVFNINMATINFCDATILKALKKSPYISKDVIDKITLDKEEKDLTNIKLFKLYIQEYLKNN
PAVYTEGFTFLVRQLEPTINGLPIEIYIFVKEVNLVGYENIQDNISEHFISILPEFKLKIFQNVGVV
>Mature_387_residues
MQYLMDLYHTSSTELVLLFVMLVSLIPVIFLIKRLIFIPVKNYLTRHHYDDYERIKKYPIFRYLLHTLLALYFVCWGKIF
HPTSFKAHVLLGIKDTIVILYTGIPVTMLLLTLIDAFADLYHNRIKAVTKNAPLSLYFQILKIIVIVIAAMVTISYILNI
SLSTFLTSLGAATALLTFVFKDTVLGLLASLQLTTQEIINIGDWVRIGAIEGTVEKITISVVTIRNFDHSISTVPTYSIL
NSNVTNYRGISESGARRVKRVFNINMATINFCDATILKALKKSPYISKDVIDKITLDKEEKDLTNIKLFKLYIQEYLKNN
PAVYTEGFTFLVRQLEPTINGLPIEIYIFVKEVNLVGYENIQDNISEHFISILPEFKLKIFQNVGVV

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=267, Percent_Identity=35.2059925093633, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 44234; Mature: 44234

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYLMDLYHTSSTELVLLFVMLVSLIPVIFLIKRLIFIPVKNYLTRHHYDDYERIKKYPI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHH
FRYLLHTLLALYFVCWGKIFHPTSFKAHVLLGIKDTIVILYTGIPVTMLLLTLIDAFADL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHH
YHNRIKAVTKNAPLSLYFQILKIIVIVIAAMVTISYILNISLSTFLTSLGAATALLTFVF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDTVLGLLASLQLTTQEIINIGDWVRIGAIEGTVEKITISVVTIRNFDHSISTVPTYSIL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCCCCCCHHHH
NSNVTNYRGISESGARRVKRVFNINMATINFCDATILKALKKSPYISKDVIDKITLDKEE
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCCH
KDLTNIKLFKLYIQEYLKNNPAVYTEGFTFLVRQLEPTINGLPIEIYIFVKEVNLVGYEN
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHH
IQDNISEHFISILPEFKLKIFQNVGVV
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQYLMDLYHTSSTELVLLFVMLVSLIPVIFLIKRLIFIPVKNYLTRHHYDDYERIKKYPI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHH
FRYLLHTLLALYFVCWGKIFHPTSFKAHVLLGIKDTIVILYTGIPVTMLLLTLIDAFADL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHH
YHNRIKAVTKNAPLSLYFQILKIIVIVIAAMVTISYILNISLSTFLTSLGAATALLTFVF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDTVLGLLASLQLTTQEIINIGDWVRIGAIEGTVEKITISVVTIRNFDHSISTVPTYSIL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEEEEEECCCCCCCCCCCHHHH
NSNVTNYRGISESGARRVKRVFNINMATINFCDATILKALKKSPYISKDVIDKITLDKEE
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCCH
KDLTNIKLFKLYIQEYLKNNPAVYTEGFTFLVRQLEPTINGLPIEIYIFVKEVNLVGYEN
HHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCHH
IQDNISEHFISILPEFKLKIFQNVGVV
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]