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The map label for this gene is hhaIM [H]

Identifier: 229586279

GI number: 229586279

Start: 61645

End: 61842

Strand: Direct

Name: hhaIM [H]

Synonym: RAF_ORF0062

Alternate gene names: 229586279

Gene position: 61645-61842 (Clockwise)

Preceding gene: 229586278

Following gene: 229586280

Centisome position: 4.82

GC content: 33.33

Gene sequence:

>198_bases
ATGTGTTTTTTCATAGACAAAGACGTACAAGAAGCATATAAAAGAAATTTCGGTGATAAGCCATACGGTGATATTACGGA
AATATCAGAAACCAAAATCCCTAAACATGATATTTTATGTGCCGGTTTTCCTTGCCAATCTTTTAGTATTTCAGGTAAGA
GGCTTGGTATAGGAGATGTAGACTTTTGTATGAAATAA

Upstream 100 bases:

>100_bases
TACGCTTCCTAAGTTTAGTAGTATAATTTATGTATAAATTTATAGATTTATTTTGTGGTATCGGTGGTTTTAGAAAAGCC
TTGGAAATAAAGGGTCTTGA

Downstream 100 bases:

>100_bases
TCAGAATAGTACAATATCACAAACCCTATATATTATTGCTTGAGAATGTTAAAAATATTCTGAATATTAATAATGGCAGC
GTAATTGAAACTAAAAAATT

Product: Site-specific DNA methylase

Products: NA

Alternate protein names: M.HhaI; Cytosine-specific methyltransferase HhaI [H]

Number of amino acids: Translated: 65; Mature: 65

Protein sequence:

>65_residues
MCFFIDKDVQEAYKRNFGDKPYGDITEISETKIPKHDILCAGFPCQSFSISGKRLGIGDVDFCMK

Sequences:

>Translated_65_residues
MCFFIDKDVQEAYKRNFGDKPYGDITEISETKIPKHDILCAGFPCQSFSISGKRLGIGDVDFCMK
>Mature_65_residues
MCFFIDKDVQEAYKRNFGDKPYGDITEISETKIPKHDILCAGFPCQSFSISGKRLGIGDVDFCMK

Specific function: This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 7356; Mature: 7356

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFFIDKDVQEAYKRNFGDKPYGDITEISETKIPKHDILCAGFPCQSFSISGKRLGIGDV
CCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
DFCMK
HHHCC
>Mature Secondary Structure
MCFFIDKDVQEAYKRNFGDKPYGDITEISETKIPKHDILCAGFPCQSFSISGKRLGIGDV
CCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
DFCMK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3549710; 8506140; 7899082; 8343957; 8293469; 9207024; 9783745; 10080885 [H]