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The map label for this gene is clpB [H]

Identifier: 229586269

GI number: 229586269

Start: 49865

End: 52438

Strand: Direct

Name: clpB [H]

Synonym: RAF_ORF0052

Alternate gene names: 229586269

Gene position: 49865-52438 (Clockwise)

Preceding gene: 229586264

Following gene: 229586274

Centisome position: 3.9

GC content: 37.02

Gene sequence:

>2574_bases
ATGAATATTGATAAATTTACTGCACATGCTAAATCAGTGATAGCTAGTAGCCAATCACTTGCTGCTAAAAATGATCATCA
GCAAATATTACCTTTGCATTTATTATCTAGTCTTTTAAGTGAAGAAACAGGTATAATTCAAACCCTTATTAATAATACTG
GTGGTAATATAAACTTATTAAAAGATCAAGTTCAGCTAGAACTAAATAAAATCCCAAAAGTTCAGGTTGAGGGAGGGGGA
CAAGTTTATTCTTCTGCTGAAGCTCTTAAAGTTTTAGAAAAAGCTAGTAGCATTGCTAAAGACAGTGGGGATAGCTTTGT
AACCATAGAGCGTATATTTGAAGCATTAACGTATGATAATACTATAGCCGGTAAAATTTTAACGAATAATGGAATTAATA
ATAAAAAAATAGCAACAGCTATTTTACAGTTTCGTAAAGGCAAAAAAGCAGATACCGAATCGGCAGAAAATAGTTACGAT
GCTTTAAAGAAATACGGCAGAGACGTAACAGAGCTTGCCGAAAGCGGTAAGCTTGATCCGATAATCGGACGTGATGAAGA
AATAAGAAGAACGGTGCAGGTACTATCACGACGTATGAAAAATAATCCTGTATTGATTGGAGAACCTGGAGTCGGTAAAA
CTGCTATAATAGAGGGGCTTGCACAACGAATATTTAGTAAGGACGTACCTGAATCGCTTATTAATTGCCGTATTATTGAG
CTTGATATGGGAGCTTTAATAGCGGGAGCTAAATATCGAGGCGAATTTGAAGAACGTCTTAAAGCAGTGCTTAGCGAAAT
CAAAGAATCAAGCGGTGAAATTATTTTATTTATCGATGAATTGCATCTATTAGTCGGTACGGGAAAAACCGACGGTGCTA
TGGATGCCTCGAATTTACTAAAGCCGATGCTTGCTCGTGGTGAGCTGCATTGTATCGGTGCTACTACTCTAGATGAATAT
CGTAAATATATCGAGAAAGATGCGGCACTTGCTCGTCGTTTTCAGCCTGTTTACGTGAGTGAGCCGACGGTTGAGGATAC
TATATCAATACTTAGAGGAATTAAAGAAAAATATGAGCTGCATCATGCAGTGCGAATTTCCGATAGTGCAATAGTTGCAG
CAGCGACGTTATCAAACCGTTATATTACCGATCGTTATTTACCTGATAAAGCTATTGATTTGATTGATGAAGCTTGTAGC
CGTATGAAAATAGAGCTATCAAGTAAACCAGAAGAGCTTGATGAGCTGGACCGTCGTATTATTCAGATAAAAGTAGAGCT
TGCAGCACTTAAAAAAGAAAATGACGAGCATTCTAAAAAGAAAATAGAGCATTTAACTACAGCATTAGAAAAATTAGAGT
CTCAATCCTATGATATGGGAGCTAAATGGCAAGCTGAAAAGTCTAAACTACAGCAAACACAAAAGCTCAAAGAAGAACTA
GACCGTAGCCGAAACGAGCTTGAACGTGCCGAACGTGATGCTAATCTCGCTAAAGCTAGTGAGCTAAAATACGGAATTAT
ACCTGAGATTATGAAAAAGATTCAGGAAGCCGAGAGTATGGATAATAAAGGGCTGTTAAAAGAAATTGTATCGGAAAGCG
ATATAGCAAGTATTATCTCTCGTATTACTGGTATTCCAATTGATACGATGCTATCAAGTGAACGTGAGCGTCTGCTTGTG
ATGGAGCAGAAATTACGAGAATCGGTAATAGGGCAGGATAAGGCAATTAAAGGTGTTAGTGATGCGGTAAGAAGATCACG
TGCAGGGATTCAAGATATTAACCGCCCGCTTGGTTCGTTCTTATTCTTAGGACCGACCGGAGTCGGTAAAACCGAGCTTA
CCAAAGCCTTAGCTGGTTTTCTTTTTGATGACCGTAATGCAATACTTCGTATAGATATGTCGGAATATATGGAGAAGCAC
GCTATTTCTCGCCTGATAGGAGCTCCTCCCGGCTATATCGGATATGATCAAGGCGGAGTGTTGACGGAGGCAGTAAGACG
CCGTCCTTATCAGGTAATATTATTTGATGAGGTTGAAAAAGCCCATCCCGACATATTTAATATTATGCTGCAAATACTTG
ATGAGGGAAGATTAACAGATAGTCAAGGTATAACGGTTGATTTTAAGAATACTATAATAGTTTTAACGTCTAATTTAGGT
GCTGAGATACTCGTTAATCAGAAAGAGGACGAAGATACATATAAAGTAAAGGATGAGGTTATGGAATATGTAAAAGCAGT
ATTTAAACCTGAGTTTTTAAACAGACTAGACGAGATTATATTATTCCATCGCCTAAATCGTAATAATATTCATGATATAG
TTAAGATACAGCTTGAAAGTTTAAAGAAAATTTTACTAGCACAAAATATTATTCTTGAATTTGATGAATCTGCATTAAAT
TATTTAGCCGAAAAAGGTTATGATCCAAGCTTTGGAGCAAGACCCTTAAAACGTCTTATCCAGCGGGAAATACAAAATAA
TTTAGCCAAAATGATTTTAGCAGGCGAAATAAGTAGCGGTAAAACGGTTAAAATAAATTCAAAGGATAAAGAATTGAAGG
TAACAATGAGTTGA

Upstream 100 bases:

>100_bases
TATGTAGTTGTGTATCTCTTGTTATTTTAATTAAAAATTATTATATAAAAATAGTCTAGTTAAAAATATTTAACTTTATT
AATTAAAATATATTATTTAT

Downstream 100 bases:

>100_bases
TTAATTTTTTCCGAATAACGAATTATCGCATAATCCACTATTCTTGTTAAAGGCACTGTTTAATAATTATTGATTTTGTC
ATTGCGAGCTATTAAAAGAT

Product: ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MNIDKFTAHAKSVIASSQSLAAKNDHQQILPLHLLSSLLSEETGIIQTLINNTGGNINLLKDQVQLELNKIPKVQVEGGG
QVYSSAEALKVLEKASSIAKDSGDSFVTIERIFEALTYDNTIAGKILTNNGINNKKIATAILQFRKGKKADTESAENSYD
ALKKYGRDVTELAESGKLDPIIGRDEEIRRTVQVLSRRMKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIE
LDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLLKPMLARGELHCIGATTLDEY
RKYIEKDAALARRFQPVYVSEPTVEDTISILRGIKEKYELHHAVRISDSAIVAAATLSNRYITDRYLPDKAIDLIDEACS
RMKIELSSKPEELDELDRRIIQIKVELAALKKENDEHSKKKIEHLTTALEKLESQSYDMGAKWQAEKSKLQQTQKLKEEL
DRSRNELERAERDANLAKASELKYGIIPEIMKKIQEAESMDNKGLLKEIVSESDIASIISRITGIPIDTMLSSERERLLV
MEQKLRESVIGQDKAIKGVSDAVRRSRAGIQDINRPLGSFLFLGPTGVGKTELTKALAGFLFDDRNAILRIDMSEYMEKH
AISRLIGAPPGYIGYDQGGVLTEAVRRRPYQVILFDEVEKAHPDIFNIMLQILDEGRLTDSQGITVDFKNTIIVLTSNLG
AEILVNQKEDEDTYKVKDEVMEYVKAVFKPEFLNRLDEIILFHRLNRNNIHDIVKIQLESLKKILLAQNIILEFDESALN
YLAEKGYDPSFGARPLKRLIQREIQNNLAKMILAGEISSGKTVKINSKDKELKVTMS

Sequences:

>Translated_857_residues
MNIDKFTAHAKSVIASSQSLAAKNDHQQILPLHLLSSLLSEETGIIQTLINNTGGNINLLKDQVQLELNKIPKVQVEGGG
QVYSSAEALKVLEKASSIAKDSGDSFVTIERIFEALTYDNTIAGKILTNNGINNKKIATAILQFRKGKKADTESAENSYD
ALKKYGRDVTELAESGKLDPIIGRDEEIRRTVQVLSRRMKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIE
LDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLLKPMLARGELHCIGATTLDEY
RKYIEKDAALARRFQPVYVSEPTVEDTISILRGIKEKYELHHAVRISDSAIVAAATLSNRYITDRYLPDKAIDLIDEACS
RMKIELSSKPEELDELDRRIIQIKVELAALKKENDEHSKKKIEHLTTALEKLESQSYDMGAKWQAEKSKLQQTQKLKEEL
DRSRNELERAERDANLAKASELKYGIIPEIMKKIQEAESMDNKGLLKEIVSESDIASIISRITGIPIDTMLSSERERLLV
MEQKLRESVIGQDKAIKGVSDAVRRSRAGIQDINRPLGSFLFLGPTGVGKTELTKALAGFLFDDRNAILRIDMSEYMEKH
AISRLIGAPPGYIGYDQGGVLTEAVRRRPYQVILFDEVEKAHPDIFNIMLQILDEGRLTDSQGITVDFKNTIIVLTSNLG
AEILVNQKEDEDTYKVKDEVMEYVKAVFKPEFLNRLDEIILFHRLNRNNIHDIVKIQLESLKKILLAQNIILEFDESALN
YLAEKGYDPSFGARPLKRLIQREIQNNLAKMILAGEISSGKTVKINSKDKELKVTMS
>Mature_857_residues
MNIDKFTAHAKSVIASSQSLAAKNDHQQILPLHLLSSLLSEETGIIQTLINNTGGNINLLKDQVQLELNKIPKVQVEGGG
QVYSSAEALKVLEKASSIAKDSGDSFVTIERIFEALTYDNTIAGKILTNNGINNKKIATAILQFRKGKKADTESAENSYD
ALKKYGRDVTELAESGKLDPIIGRDEEIRRTVQVLSRRMKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIE
LDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLLKPMLARGELHCIGATTLDEY
RKYIEKDAALARRFQPVYVSEPTVEDTISILRGIKEKYELHHAVRISDSAIVAAATLSNRYITDRYLPDKAIDLIDEACS
RMKIELSSKPEELDELDRRIIQIKVELAALKKENDEHSKKKIEHLTTALEKLESQSYDMGAKWQAEKSKLQQTQKLKEEL
DRSRNELERAERDANLAKASELKYGIIPEIMKKIQEAESMDNKGLLKEIVSESDIASIISRITGIPIDTMLSSERERLLV
MEQKLRESVIGQDKAIKGVSDAVRRSRAGIQDINRPLGSFLFLGPTGVGKTELTKALAGFLFDDRNAILRIDMSEYMEKH
AISRLIGAPPGYIGYDQGGVLTEAVRRRPYQVILFDEVEKAHPDIFNIMLQILDEGRLTDSQGITVDFKNTIIVLTSNLG
AEILVNQKEDEDTYKVKDEVMEYVKAVFKPEFLNRLDEIILFHRLNRNNIHDIVKIQLESLKKILLAQNIILEFDESALN
YLAEKGYDPSFGARPLKRLIQREIQNNLAKMILAGEISSGKTVKINSKDKELKVTMS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=327, Percent_Identity=34.8623853211009, Blast_Score=206, Evalue=1e-52,
Organism=Escherichia coli, GI1788943, Length=849, Percent_Identity=56.301531213192, Blast_Score=970, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=332, Percent_Identity=45.1807228915663, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=769, Percent_Identity=50.4551365409623, Blast_Score=780, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=861, Percent_Identity=44.1347270615563, Blast_Score=688, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95907; Mature: 95907

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDKFTAHAKSVIASSQSLAAKNDHQQILPLHLLSSLLSEETGIIQTLINNTGGNINLL
CCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEE
KDQVQLELNKIPKVQVEGGGQVYSSAEALKVLEKASSIAKDSGDSFVTIERIFEALTYDN
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCC
TIAGKILTNNGINNKKIATAILQFRKGKKADTESAENSYDALKKYGRDVTELAESGKLDP
CEECEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHCCHHHHHHHCCCCCC
IIGRDEEIRRTVQVLSRRMKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIE
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCEEEE
LDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLL
EECCHHHCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCHHHHH
KPMLARGELHCIGATTLDEYRKYIEKDAALARRFQPVYVSEPTVEDTISILRGIKEKYEL
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
HHAVRISDSAIVAAATLSNRYITDRYLPDKAIDLIDEACSRMKIELSSKPEELDELDRRI
HHHHEECCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
IQIKVELAALKKENDEHSKKKIEHLTTALEKLESQSYDMGAKWQAEKSKLQQTQKLKEEL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DRSRNELERAERDANLAKASELKYGIIPEIMKKIQEAESMDNKGLLKEIVSESDIASIIS
HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHH
RITGIPIDTMLSSERERLLVMEQKLRESVIGQDKAIKGVSDAVRRSRAGIQDINRPLGSF
HHCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCE
LFLGPTGVGKTELTKALAGFLFDDRNAILRIDMSEYMEKHAISRLIGAPPGYIGYDQGGV
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEVEKAHPDIFNIMLQILDEGRLTDSQGITVDFKNTIIVLTSNLG
HHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCC
AEILVNQKEDEDTYKVKDEVMEYVKAVFKPEFLNRLDEIILFHRLNRNNIHDIVKIQLES
CEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKKILLAQNIILEFDESALNYLAEKGYDPSFGARPLKRLIQREIQNNLAKMILAGEISSG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCC
KTVKINSKDKELKVTMS
CEEEECCCCCCEEEEEC
>Mature Secondary Structure
MNIDKFTAHAKSVIASSQSLAAKNDHQQILPLHLLSSLLSEETGIIQTLINNTGGNINLL
CCCHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEE
KDQVQLELNKIPKVQVEGGGQVYSSAEALKVLEKASSIAKDSGDSFVTIERIFEALTYDN
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCC
TIAGKILTNNGINNKKIATAILQFRKGKKADTESAENSYDALKKYGRDVTELAESGKLDP
CEECEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHCCHHHHHHHCCCCCC
IIGRDEEIRRTVQVLSRRMKNNPVLIGEPGVGKTAIIEGLAQRIFSKDVPESLINCRIIE
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCEEEE
LDMGALIAGAKYRGEFEERLKAVLSEIKESSGEIILFIDELHLLVGTGKTDGAMDASNLL
EECCHHHCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCHHHHH
KPMLARGELHCIGATTLDEYRKYIEKDAALARRFQPVYVSEPTVEDTISILRGIKEKYEL
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
HHAVRISDSAIVAAATLSNRYITDRYLPDKAIDLIDEACSRMKIELSSKPEELDELDRRI
HHHHEECCCCEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
IQIKVELAALKKENDEHSKKKIEHLTTALEKLESQSYDMGAKWQAEKSKLQQTQKLKEEL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DRSRNELERAERDANLAKASELKYGIIPEIMKKIQEAESMDNKGLLKEIVSESDIASIIS
HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHH
RITGIPIDTMLSSERERLLVMEQKLRESVIGQDKAIKGVSDAVRRSRAGIQDINRPLGSF
HHCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCE
LFLGPTGVGKTELTKALAGFLFDDRNAILRIDMSEYMEKHAISRLIGAPPGYIGYDQGGV
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEVEKAHPDIFNIMLQILDEGRLTDSQGITVDFKNTIIVLTSNLG
HHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCC
AEILVNQKEDEDTYKVKDEVMEYVKAVFKPEFLNRLDEIILFHRLNRNNIHDIVKIQLES
CEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKKILLAQNIILEFDESALNYLAEKGYDPSFGARPLKRLIQREIQNNLAKMILAGEISSG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCC
KTVKINSKDKELKVTMS
CEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA