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The map label for this gene is rsmE [H]

Identifier: 229259630

GI number: 229259630

Start: 5679733

End: 5680467

Strand: Reverse

Name: rsmE [H]

Synonym: mll6903

Alternate gene names: 229259630

Gene position: 5680467-5679733 (Counterclockwise)

Preceding gene: 13475750

Following gene: 13475748

Centisome position: 80.73

GC content: 62.86

Gene sequence:

>735_bases
ATGCGCGCCAATTACAAGATGCAACGTCTGTTCGTGCCTGACGATCTCGGGCCGGATGCCGAGTTCGACGCCGACCAGCA
GCAGAGCCACTATCTCATTCATGTGCTGCGGCTTGGCGAAGGTGCCGAAATCCTGCTGTTCAATGGCCGCGACGGTGAAT
GGTCGGCGGCCATCGCGGCGAAATCTAAGAAAGCGGTGAGGCTTAGGGTACAGACCCTGCAGCGGCCGCAGCCACCTCTC
CCCGATCTCATCTATTGTTTCGCGCCGCTGAAGCAGGGGAGGCTCGATTATCTCGTGCAGAAGGCGGTCGAAATGGGCGC
GGGCATTTTGCAGCCCGTTATCACGCAGCACACGCAAGTGGCCAAGCCTTCCATCGATCGGCTGCGCGCCAATGTCGTCG
AGGCCGCCGAACAATGCGGCATACTGGCGGTTCCGGAAGTGCGAGAAGCAGAAAAATTCGAACGCCTGCTGGCCGGCTGG
GACAAGGAAAGGCGGCTGGTCTTCTGCGACGAGGACGCGTCGACCAACAATCCGCTGCCAGCCTTGCGGGCAGTGAAGGA
GAAGAAGCTCGCGCTGCTGGTCGGGCCGGAGGGCGGCTTTTCCGATGACGAGCGCAAGATGCTGCGCGCCCTGCCCTTCG
TGACCGCCATTCCGCTCGGCCCACGCATCCTGCGCGCCGACACGGCGGCGGTGGCCGCCCTTGCAGTGATGCAGGCAACC
GTCGGAGACTGGTAG

Upstream 100 bases:

>100_bases
CCGGGGACGACACTAGCCGTCCTGCGGCACGGTTTTCGGCCGGTGTGGCATCCATCCGCCAAGGCTTGACGCCAGCGGCC
GGCTCGCTCATTCCTCGGCC

Downstream 100 bases:

>100_bases
GACGTCATGTCTTGCAAGAGGCCGCCCGGATCGTGGCCTTCAATTCCTCTTGACCTGTGGCTCAGGATTTTTCGCTTGAT
CGGCTACTGACATTTCGTCC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRANYKMQRLFVPDDLGPDAEFDADQQQSHYLIHVLRLGEGAEILLFNGRDGEWSAAIAAKSKKAVRLRVQTLQRPQPPL
PDLIYCFAPLKQGRLDYLVQKAVEMGAGILQPVITQHTQVAKPSIDRLRANVVEAAEQCGILAVPEVREAEKFERLLAGW
DKERRLVFCDEDASTNNPLPALRAVKEKKLALLVGPEGGFSDDERKMLRALPFVTAIPLGPRILRADTAAVAALAVMQAT
VGDW

Sequences:

>Translated_244_residues
MRANYKMQRLFVPDDLGPDAEFDADQQQSHYLIHVLRLGEGAEILLFNGRDGEWSAAIAAKSKKAVRLRVQTLQRPQPPL
PDLIYCFAPLKQGRLDYLVQKAVEMGAGILQPVITQHTQVAKPSIDRLRANVVEAAEQCGILAVPEVREAEKFERLLAGW
DKERRLVFCDEDASTNNPLPALRAVKEKKLALLVGPEGGFSDDERKMLRALPFVTAIPLGPRILRADTAAVAALAVMQAT
VGDW
>Mature_244_residues
MRANYKMQRLFVPDDLGPDAEFDADQQQSHYLIHVLRLGEGAEILLFNGRDGEWSAAIAAKSKKAVRLRVQTLQRPQPPL
PDLIYCFAPLKQGRLDYLVQKAVEMGAGILQPVITQHTQVAKPSIDRLRANVVEAAEQCGILAVPEVREAEKFERLLAGW
DKERRLVFCDEDASTNNPLPALRAVKEKKLALLVGPEGGFSDDERKMLRALPFVTAIPLGPRILRADTAAVAALAVMQAT
VGDW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=246, Percent_Identity=30.0813008130081, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27014; Mature: 27014

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANYKMQRLFVPDDLGPDAEFDADQQQSHYLIHVLRLGEGAEILLFNGRDGEWSAAIAA
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHH
KSKKAVRLRVQTLQRPQPPLPDLIYCFAPLKQGRLDYLVQKAVEMGAGILQPVITQHTQV
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKPSIDRLRANVVEAAEQCGILAVPEVREAEKFERLLAGWDKERRLVFCDEDASTNNPLP
CCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCH
ALRAVKEKKLALLVGPEGGFSDDERKMLRALPFVTAIPLGPRILRADTAAVAALAVMQAT
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VGDW
HCCC
>Mature Secondary Structure
MRANYKMQRLFVPDDLGPDAEFDADQQQSHYLIHVLRLGEGAEILLFNGRDGEWSAAIAA
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHH
KSKKAVRLRVQTLQRPQPPLPDLIYCFAPLKQGRLDYLVQKAVEMGAGILQPVITQHTQV
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
AKPSIDRLRANVVEAAEQCGILAVPEVREAEKFERLLAGWDKERRLVFCDEDASTNNPLP
CCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCH
ALRAVKEKKLALLVGPEGGFSDDERKMLRALPFVTAIPLGPRILRADTAAVAALAVMQAT
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VGDW
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]