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The map label for this gene is dapF

Identifier: 228963958

GI number: 228963958

Start: 2454548

End: 2455387

Strand: Direct

Name: dapF

Synonym: alr2048

Alternate gene names: 228963958

Gene position: 2454548-2455387 (Clockwise)

Preceding gene: 17229538

Following gene: 17229541

Centisome position: 38.27

GC content: 46.31

Gene sequence:

>840_bases
ATGGCAATCGAATTTACTAAGTATCACGGTCTAGGCAACGATTTTATTCTCATCGATAATCGCGCTTCTAAAACGCCGGC
AATCACTCCAGAGAAAGCGGTGGAGATGTGCGATCGCCACTTTGGTATCGGGGCTGATGGTGTAATTTTTGCCTTACCTG
GAGAAAACGGTACTGATTACACCATGCGGATTTTTAATTCCGATGGTTCAGAACCGGAAATGTGTGGTAATGGTATTCGC
TGTTTAGCTGCATTTCTGGCTGATTTGGAAGGGCTTTCTCGTAATAAAGATACCTATCGTATTCATACCTTGGCTGGTGT
GATTACCCCCCAACTCACCCCTGATGGACAAATCAAGGTGGATATGGGTTTACCTCGGTTACTAGCTGGGGAAATTCCGA
CAACTCTTGGTGCTGCTGATGGGAAAGTCATTAACCAACCCCTAGAAGTAGAAGGGCAAACTTGGGAAGTTACTTGTGTC
AGCATGGGGAATCCCCACTGTATTACTTTTGTCGAAGACGTGGCGGCGATTCCTTTAGAAATCATCGGCCCTAAATTTGA
ACATCACCCAGCATTTCCCCAACGGACAAACACCGAATTTATTCAAGTTGTCAGCCGTGACTATTTAAAGATGCGTGTTT
GGGAACGTGGTGCAGGAATTACTTTAGCTTGCGGTACAGGGGCTTGTGCTTCCTTGGTAGCGGCGGTATTGACTGGAAGA
AGCGATCGCCTCGCCACTGTAGAATTACCTGGTGGCCCCTTGGAAATTGAATGGTCAGAAGTAGACCAAAGAATTTATAT
GACCGGCCCGGCTGATAGGGTATTCACAGGTAAACTTTGA

Upstream 100 bases:

>100_bases
AGTCATAGGTAATAGGTAATGGGTAATGGGTAGTGGATGAAAATTTATTCTCAATTACCAATCACCAATACCTAATCATT
GATAATTCATCATGGGAAAA

Downstream 100 bases:

>100_bases
GCAAGATTCCTTTCACCCTTCCCATAGGTGTGCCTTTGTTGGAGACTCACATTAAATCAACCAACCCCAGGTTGTGGTAG
TTTGATTTACAGATGTATAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase 2

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGNGIR
CLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTTLGAADGKVINQPLEVEGQTWEVTCV
SMGNPHCITFVEDVAAIPLEIIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLTGR
SDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL

Sequences:

>Translated_279_residues
MAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGNGIR
CLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTTLGAADGKVINQPLEVEGQTWEVTCV
SMGNPHCITFVEDVAAIPLEIIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLTGR
SDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
>Mature_278_residues
AIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDYTMRIFNSDGSEPEMCGNGIRC
LAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKVDMGLPRLLAGEIPTTLGAADGKVINQPLEVEGQTWEVTCVS
MGNPHCITFVEDVAAIPLEIIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLTGRS
DRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=276, Percent_Identity=41.304347826087, Blast_Score=211, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF2_NOSS1 (Q8YVD0)

Other databases:

- EMBL:   BA000019
- PIR:   AB2062
- RefSeq:   NP_486088.2
- ProteinModelPortal:   Q8YVD0
- SMR:   Q8YVD0
- STRING:   Q8YVD0
- GeneID:   1105643
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr2048
- NMPDR:   fig|103690.1.peg.2355
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q8YVD0
- ProtClustDB:   PRK00450
- BioCyc:   NSP103690:ALR2048-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30233; Mature: 30102

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDY
CCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
TMRIFNSDGSEPEMCGNGIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKV
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCEEE
DMGLPRLLAGEIPTTLGAADGKVINQPLEVEGQTWEVTCVSMGNPHCITFVEDVAAIPLE
CCCCCHHHHCCCCCCCCCCCCCEECCCEECCCCEEEEEEEECCCCEEEEEEHHHHHCCHH
IIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLTGR
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHHCCC
SDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
CCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCEEECCC
>Mature Secondary Structure 
AIEFTKYHGLGNDFILIDNRASKTPAITPEKAVEMCDRHFGIGADGVIFALPGENGTDY
CEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
TMRIFNSDGSEPEMCGNGIRCLAAFLADLEGLSRNKDTYRIHTLAGVITPQLTPDGQIKV
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCEEE
DMGLPRLLAGEIPTTLGAADGKVINQPLEVEGQTWEVTCVSMGNPHCITFVEDVAAIPLE
CCCCCHHHHCCCCCCCCCCCCCEECCCEECCCCEEEEEEEECCCCEEEEEEHHHHHCCHH
IIGPKFEHHPAFPQRTNTEFIQVVSRDYLKMRVWERGAGITLACGTGACASLVAAVLTGR
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHHHCCC
SDRLATVELPGGPLEIEWSEVDQRIYMTGPADRVFTGKL
CCCEEEEECCCCCEEEEHHHCCCEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840