Click here to switch to the map view.

The map label for this gene is yjjN [C]

Identifier: 227828675

GI number: 227828675

Start: 2256519

End: 2257451

Strand: Direct

Name: yjjN [C]

Synonym: M1425_2432

Alternate gene names: 227828675

Gene position: 2256519-2257451 (Clockwise)

Preceding gene: 227828674

Following gene: 227828676

Centisome position: 86.5

GC content: 39.12

Gene sequence:

>933_bases
ATGAAAGCGGCTATTCTCACACAGTTTAACTCACCTTTTGTTATAGGTAACGCTGAGCCTAAAGGGGTTGGAGTTAAAGT
AAATGTAGCATATGCTGGAATATGCGGAAGAGATCTAGTTATATGGAAGGGTGGATTTAAAAACCTTAAATTGCCCTTAA
TCTTAGGACATGAAATAGTAGGCTACTATAAGGGAAGACCTGTGGCGGTTTATCCAAACCTGAATTGCGGAAGCTGTGAG
TACTGCAAAAGTGGGAAGGAGAATCTGTGCGACAATGCTAGAATTTTAGGAGAAGGAGAGATAACTGGAGGGTATGCTGA
GGAGGTTATAATTCCAGAAAGAAACATTATACCATTACCAGACGATAAATTAGAAAAATACGCCGCAACCATGGACCCCG
TAGCAACTGCAATTCACGCAACTAAGCTTGCAAATTTAAATAGAGATAGTAAAGTTTTAGTAACTGGTGCAGGTGGAGGA
GTGGGTATTCATCTCGTACAATATTTGAAATATTTCGGTATTTCTAGTGTTTATGGGCTATCGTCTAAAGGGGAAAAGCT
ACAAGAATTGGGAGTAACGCCCATTAGTGATATAAGAAGTGAGAAGTTTGATACAATATTTGAGCTGGTAGGCTCTAAAA
CAATAAACGATTCCTTGAGGGCACTAAAGAAGGAGGGTACTCTAGTTTTGATAGGAAATACTGAAGGCGAGCCAATAACG
TTGTTAAGGCCAGCCATGACTATAATGAGACAACAAAAAATTATAGGTTCGGCATCTTATACTAAGGCAGAGTACGAGGA
GGCTATTAGATTAGTGGGAGAAGGGAAAATAAAAGCGATATATGAGATTTACGAACTAGAGAGGATAAATGAGGCGTATA
GGAAAATGATGGAGAGAAAGGTTCTGGGAAGAGCTGTATTAAAGGTAATTTAA

Upstream 100 bases:

>100_bases
GATGACGCTTTAGCTGGAAAGATTGAGGATAAGTGTATGTTTGTACCTAAGTCTTTAAGTCAACAATGAATATATTAACA
ATCAATTCCCTTTTTAATAC

Downstream 100 bases:

>100_bases
CCTTCTTTATACATGTAATTCTCATAAGGTGATAACCTAAATATTATATTTTTACGTTGACCTTTCTTTAATTCATCATG
AGTAACGTATTAGTTTTTGA

Product: alcohol dehydrogenase GroES domain protein

Products: NA

Alternate protein names: Acryloyl-CoA reductase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKAAILTQFNSPFVIGNAEPKGVGVKVNVAYAGICGRDLVIWKGGFKNLKLPLILGHEIVGYYKGRPVAVYPNLNCGSCE
YCKSGKENLCDNARILGEGEITGGYAEEVIIPERNIIPLPDDKLEKYAATMDPVATAIHATKLANLNRDSKVLVTGAGGG
VGIHLVQYLKYFGISSVYGLSSKGEKLQELGVTPISDIRSEKFDTIFELVGSKTINDSLRALKKEGTLVLIGNTEGEPIT
LLRPAMTIMRQQKIIGSASYTKAEYEEAIRLVGEGKIKAIYEIYELERINEAYRKMMERKVLGRAVLKVI

Sequences:

>Translated_310_residues
MKAAILTQFNSPFVIGNAEPKGVGVKVNVAYAGICGRDLVIWKGGFKNLKLPLILGHEIVGYYKGRPVAVYPNLNCGSCE
YCKSGKENLCDNARILGEGEITGGYAEEVIIPERNIIPLPDDKLEKYAATMDPVATAIHATKLANLNRDSKVLVTGAGGG
VGIHLVQYLKYFGISSVYGLSSKGEKLQELGVTPISDIRSEKFDTIFELVGSKTINDSLRALKKEGTLVLIGNTEGEPIT
LLRPAMTIMRQQKIIGSASYTKAEYEEAIRLVGEGKIKAIYEIYELERINEAYRKMMERKVLGRAVLKVI
>Mature_310_residues
MKAAILTQFNSPFVIGNAEPKGVGVKVNVAYAGICGRDLVIWKGGFKNLKLPLILGHEIVGYYKGRPVAVYPNLNCGSCE
YCKSGKENLCDNARILGEGEITGGYAEEVIIPERNIIPLPDDKLEKYAATMDPVATAIHATKLANLNRDSKVLVTGAGGG
VGIHLVQYLKYFGISSVYGLSSKGEKLQELGVTPISDIRSEKFDTIFELVGSKTINDSLRALKKEGTLVLIGNTEGEPIT
LLRPAMTIMRQQKIIGSASYTKAEYEEAIRLVGEGKIKAIYEIYELERINEAYRKMMERKVLGRAVLKVI

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the acryloyl- CoA dependent NADPH oxidation and formation of propionyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI226510992, Length=299, Percent_Identity=26.7558528428094, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790718, Length=306, Percent_Identity=27.7777777777778, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787863, Length=206, Percent_Identity=31.5533980582524, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788407, Length=321, Percent_Identity=26.1682242990654, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788073, Length=318, Percent_Identity=25.7861635220126, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786518, Length=329, Percent_Identity=26.7477203647416, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081918, Length=304, Percent_Identity=26.9736842105263, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790045, Length=202, Percent_Identity=27.2277227722772, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082401, Length=314, Percent_Identity=26.4331210191083, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988145, Length=324, Percent_Identity=27.4691358024691, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562876, Length=316, Percent_Identity=26.2658227848101, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562584, Length=341, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=339, Percent_Identity=25.9587020648968, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=182, Percent_Identity=30.2197802197802, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=344, Percent_Identity=27.6162790697674, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324486, Length=319, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=319, Percent_Identity=27.8996865203762, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=322, Percent_Identity=27.9503105590062, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=311, Percent_Identity=26.3665594855305, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=311, Percent_Identity=26.3665594855305, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=322, Percent_Identity=27.9503105590062, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319257, Length=309, Percent_Identity=24.5954692556634, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323099, Length=312, Percent_Identity=24.6794871794872, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=214, Percent_Identity=32.2429906542056, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17137530, Length=218, Percent_Identity=27.5229357798165, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.3.1.84 [H]

Molecular weight: Translated: 33915; Mature: 33915

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAILTQFNSPFVIGNAEPKGVGVKVNVAYAGICGRDLVIWKGGFKNLKLPLILGHEIV
CCCEEEEECCCCEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCHHH
GYYKGRPVAVYPNLNCGSCEYCKSGKENLCDNARILGEGEITGGYAEEVIIPERNIIPLP
HEECCCCEEEECCCCCCCCHHHHCCCHHCCCCCEEEECCCCCCCCCCEEECCCCCEECCC
DDKLEKYAATMDPVATAIHATKLANLNRDSKVLVTGAGGGVGIHLVQYLKYFGISSVYGL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
SSKGEKLQELGVTPISDIRSEKFDTIFELVGSKTINDSLRALKKEGTLVLIGNTEGEPIT
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEE
LLRPAMTIMRQQKIIGSASYTKAEYEEAIRLVGEGKIKAIYEIYELERINEAYRKMMERK
EHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLGRAVLKVI
HHHHHHHHCC
>Mature Secondary Structure
MKAAILTQFNSPFVIGNAEPKGVGVKVNVAYAGICGRDLVIWKGGFKNLKLPLILGHEIV
CCCEEEEECCCCEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCHHH
GYYKGRPVAVYPNLNCGSCEYCKSGKENLCDNARILGEGEITGGYAEEVIIPERNIIPLP
HEECCCCEEEECCCCCCCCHHHHCCCHHCCCCCEEEECCCCCCCCCCEEECCCCCEECCC
DDKLEKYAATMDPVATAIHATKLANLNRDSKVLVTGAGGGVGIHLVQYLKYFGISSVYGL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
SSKGEKLQELGVTPISDIRSEKFDTIFELVGSKTINDSLRALKKEGTLVLIGNTEGEPIT
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEE
LLRPAMTIMRQQKIIGSASYTKAEYEEAIRLVGEGKIKAIYEIYELERINEAYRKMMERK
EHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLGRAVLKVI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA