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The map label for this gene is gsiB [H]

Identifier: 227828637

GI number: 227828637

Start: 2218248

End: 2220305

Strand: Direct

Name: gsiB [H]

Synonym: M1425_2390

Alternate gene names: 227828637

Gene position: 2218248-2220305 (Clockwise)

Preceding gene: 227828636

Following gene: 227828638

Centisome position: 85.03

GC content: 36.69

Gene sequence:

>2058_bases
ATGAAGTCTCGTAAGGTATTTAAGGGCTTAAGTAGAACCTTTATAGCAATAATTGTTGTTGTAATTGTTGTAATTGGAAT
AGCTGTTGGAGTATTATTAGGTAATCACCCATCTAGTAATAATATAAGCACTACTACAACTTCTTCTAATATTATCTCTA
CAACTACCCTATCATCAACATCTTCCTCAACATCTACTCCTAATAGTGTTTCAATACCTTCTAGTATTACCGTGGAAGAA
GCTGCAACACCAGTAAGTGTAGATCCGGCAAGTAGCTATGATATAGCAGGTGGGGAAATTATTCAGAATGTTTATCAAAC
TTTAGTATTTTATAATGGTACTAATACATCTTCCTTTATTGGAGTTTTGGCAGAAAATTGGACTGTAGAGAACAATGGTA
CTACTTATATCTTCCATCTATGGCCCTTTATTACTTTCAGTAATGGTAATCCTCTAAACGCTACAGATGTATGGTTTTCA
ATATACAGGACGATGCTTATTAATTTAGGTATATCAATTTATACTAGTCAAGCTTTAGCTGTTAATAATGGTCTTGGTTT
TGTAGGAAAATTACCCAACGGGAAATACGGTACAATAATGCTACCTAATGGCATACTACAAGCATTAGAGTCTGCTGGAT
ATAATTTTTCGTCAAATAAAACTATTGCGATGGAACAAGCTGCATATGATTTGGCATACATTTTATCCCATTTTAATGTA
AGCAATACCACAATTCAGAAGGTAATGTCATATCCTCATCAGGCAGTAGTAGTTATCGATCCATATACCGTAGAGTTTAA
TTTAGATTACCCATATTCAGCATTTCTAGCTGCTCTTTCCACAAGTACTGGGGCTATAGTAAATCCAGTTTTTGTTGATG
AGAATGGCGGAGTTCAAATTGATACTTCTAATACTTATCTTTCCACTCATGCTTTAGGCTCTGGACCCTATATTCTGGAA
ACTCCAATAGGAGGGTCTTACGTAGTGCTAAATGCTAGTCCTAACTATTGGGCAAGTAAAGTCCCTACAAAAGATCTAAA
TCCGATGTTGGAGACACCTAAGATTAAGACGATAATTATAGATTATCAAACTAACGAGGCAGTAAGAATATTAGACTTAC
AGCAAGAGAAGGCACAAATTTCGCAGATTGACGTGATAGACTTACAGGAGCTAATAGGTAGCTCTGGTGTGCAACAACTT
CAGAATCTGGTTAATGGAAAGACGTTCCCAACAGCCTATACCAGTGGTAATGTGACAATTTATATTTGGGGGCCTTCAGC
ACAAATAGACTTCTTGGCAATAGATGCATATCAATACCCATTTAACATAACAGCAGTAAGATTGGCCATATCACATGCAA
TAAACCCTGTACAAATTCAACAACAAGTCTATAAGGGCTTTGCAATAAACTATGTTGGACCTTTAGATCCATCATTACCA
TACTATAACTCATCAATAATAGGCTATACGTATAATCCCTCCCTTTCAATACAACTACTAGAAGAAGCAGGATTCAAATT
AACACTACCTAATGGAACTACAGTAAATCCAAATGGAAAACCTTTCCCAACAATTACCTTAACATATCAAACTGGTAGTA
CAGCTCTACAAGATGAGGCATTACTGGTTCAACAACAGCTAGCTCAAATAGGAATAACAGTTCAGCTAAATCCTGAATCC
GCGGTAACAATAGTGGAATCGTATCTAAATCCACCCAATTCATCAGCATATCCTGCCTTCCAATTAGCCGGTAACTTCCC
TCCAGTGCTGAGCCCCATAGACCCAGCAATATACTTACTGTCTCAAGCTAGATTACACCACGGAAATCCAGCTTTCGTAG
ATAATAGTACGATTAATCAGTTAATCGTAGAGGCTGTAAGAACCAATAACCCCCAGGAATTACAACATATATATAATGAA
ATAACTTTACTAACCTTAGCACAAGCACAGTATGTATGGTTAGATGACTTTTTAGCCTATACGGTAGCATCGTCAAGTAT
TCATGGATTCTGGTATAGCCCCGGATTAGATGGGTTATTCTATGCTGACTTATACTGA

Upstream 100 bases:

>100_bases
ATAGTATTTTAAATAATTTAACTAAGGTCCTTTAAAATTAAAAAGTTTTTTAAGATGAACTAAATAAGGCTTTTTTATTA
CTTTTGGAGATAAACTTCTT

Downstream 100 bases:

>100_bases
AAAGATAGTGGGGTGTAAATTTCAATATGATTTTTTATAAATTCTTGATTAAGAGAGCTATTGAGGCTTTCTTAGTAATA
ATGGCTGAACTTATTATAGT

Product: extracellular solute-binding protein family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MKSRKVFKGLSRTFIAIIVVVIVVIGIAVGVLLGNHPSSNNISTTTTSSNIISTTTLSSTSSSTSTPNSVSIPSSITVEE
AATPVSVDPASSYDIAGGEIIQNVYQTLVFYNGTNTSSFIGVLAENWTVENNGTTYIFHLWPFITFSNGNPLNATDVWFS
IYRTMLINLGISIYTSQALAVNNGLGFVGKLPNGKYGTIMLPNGILQALESAGYNFSSNKTIAMEQAAYDLAYILSHFNV
SNTTIQKVMSYPHQAVVVIDPYTVEFNLDYPYSAFLAALSTSTGAIVNPVFVDENGGVQIDTSNTYLSTHALGSGPYILE
TPIGGSYVVLNASPNYWASKVPTKDLNPMLETPKIKTIIIDYQTNEAVRILDLQQEKAQISQIDVIDLQELIGSSGVQQL
QNLVNGKTFPTAYTSGNVTIYIWGPSAQIDFLAIDAYQYPFNITAVRLAISHAINPVQIQQQVYKGFAINYVGPLDPSLP
YYNSSIIGYTYNPSLSIQLLEEAGFKLTLPNGTTVNPNGKPFPTITLTYQTGSTALQDEALLVQQQLAQIGITVQLNPES
AVTIVESYLNPPNSSAYPAFQLAGNFPPVLSPIDPAIYLLSQARLHHGNPAFVDNSTINQLIVEAVRTNNPQELQHIYNE
ITLLTLAQAQYVWLDDFLAYTVASSSIHGFWYSPGLDGLFYADLY

Sequences:

>Translated_685_residues
MKSRKVFKGLSRTFIAIIVVVIVVIGIAVGVLLGNHPSSNNISTTTTSSNIISTTTLSSTSSSTSTPNSVSIPSSITVEE
AATPVSVDPASSYDIAGGEIIQNVYQTLVFYNGTNTSSFIGVLAENWTVENNGTTYIFHLWPFITFSNGNPLNATDVWFS
IYRTMLINLGISIYTSQALAVNNGLGFVGKLPNGKYGTIMLPNGILQALESAGYNFSSNKTIAMEQAAYDLAYILSHFNV
SNTTIQKVMSYPHQAVVVIDPYTVEFNLDYPYSAFLAALSTSTGAIVNPVFVDENGGVQIDTSNTYLSTHALGSGPYILE
TPIGGSYVVLNASPNYWASKVPTKDLNPMLETPKIKTIIIDYQTNEAVRILDLQQEKAQISQIDVIDLQELIGSSGVQQL
QNLVNGKTFPTAYTSGNVTIYIWGPSAQIDFLAIDAYQYPFNITAVRLAISHAINPVQIQQQVYKGFAINYVGPLDPSLP
YYNSSIIGYTYNPSLSIQLLEEAGFKLTLPNGTTVNPNGKPFPTITLTYQTGSTALQDEALLVQQQLAQIGITVQLNPES
AVTIVESYLNPPNSSAYPAFQLAGNFPPVLSPIDPAIYLLSQARLHHGNPAFVDNSTINQLIVEAVRTNNPQELQHIYNE
ITLLTLAQAQYVWLDDFLAYTVASSSIHGFWYSPGLDGLFYADLY
>Mature_685_residues
MKSRKVFKGLSRTFIAIIVVVIVVIGIAVGVLLGNHPSSNNISTTTTSSNIISTTTLSSTSSSTSTPNSVSIPSSITVEE
AATPVSVDPASSYDIAGGEIIQNVYQTLVFYNGTNTSSFIGVLAENWTVENNGTTYIFHLWPFITFSNGNPLNATDVWFS
IYRTMLINLGISIYTSQALAVNNGLGFVGKLPNGKYGTIMLPNGILQALESAGYNFSSNKTIAMEQAAYDLAYILSHFNV
SNTTIQKVMSYPHQAVVVIDPYTVEFNLDYPYSAFLAALSTSTGAIVNPVFVDENGGVQIDTSNTYLSTHALGSGPYILE
TPIGGSYVVLNASPNYWASKVPTKDLNPMLETPKIKTIIIDYQTNEAVRILDLQQEKAQISQIDVIDLQELIGSSGVQQL
QNLVNGKTFPTAYTSGNVTIYIWGPSAQIDFLAIDAYQYPFNITAVRLAISHAINPVQIQQQVYKGFAINYVGPLDPSLP
YYNSSIIGYTYNPSLSIQLLEEAGFKLTLPNGTTVNPNGKPFPTITLTYQTGSTALQDEALLVQQQLAQIGITVQLNPES
AVTIVESYLNPPNSSAYPAFQLAGNFPPVLSPIDPAIYLLSQARLHHGNPAFVDNSTINQLIVEAVRTNNPQELQHIYNE
ITLLTLAQAQYVWLDDFLAYTVASSSIHGFWYSPGLDGLFYADLY

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=436, Percent_Identity=24.5412844036697, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787762, Length=426, Percent_Identity=21.830985915493, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789966, Length=309, Percent_Identity=20.7119741100324, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789887, Length=354, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 74414; Mature: 74414

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRKVFKGLSRTFIAIIVVVIVVIGIAVGVLLGNHPSSNNISTTTTSSNIISTTTLSST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCC
SSSTSTPNSVSIPSSITVEEAATPVSVDPASSYDIAGGEIIQNVYQTLVFYNGTNTSSFI
CCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEE
GVLAENWTVENNGTTYIFHLWPFITFSNGNPLNATDVWFSIYRTMLINLGISIYTSQALA
EEEECCEEEECCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCEEE
VNNGLGFVGKLPNGKYGTIMLPNGILQALESAGYNFSSNKTIAMEQAAYDLAYILSHFNV
EECCCCEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
SNTTIQKVMSYPHQAVVVIDPYTVEFNLDYPYSAFLAALSTSTGAIVNPVFVDENGGVQI
CHHHHHHHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEE
DTSNTYLSTHALGSGPYILETPIGGSYVVLNASPNYWASKVPTKDLNPMLETPKIKTIII
ECCCCEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
DYQTNEAVRILDLQQEKAQISQIDVIDLQELIGSSGVQQLQNLVNGKTFPTAYTSGNVTI
EECCCCEEEEEECCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEEE
YIWGPSAQIDFLAIDAYQYPFNITAVRLAISHAINPVQIQQQVYKGFAINYVGPLDPSLP
EEECCCCCEEEEEEEEEECCCCHHHEEEEHHHCCCHHHHHHHHHCCEEEEEECCCCCCCC
YYNSSIIGYTYNPSLSIQLLEEAGFKLTLPNGTTVNPNGKPFPTITLTYQTGSTALQDEA
CCCCEEEEEEECCCEEEEEEECCCCEEECCCCCEECCCCCCCCEEEEEEECCCCCHHHHH
LLVQQQLAQIGITVQLNPESAVTIVESYLNPPNSSAYPAFQLAGNFPPVLSPIDPAIYLL
HHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
SQARLHHGNPAFVDNSTINQLIVEAVRTNNPQELQHIYNEITLLTLAQAQYVWLDDFLAY
HHHHHCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCEEEEHHHHHHH
TVASSSIHGFWYSPGLDGLFYADLY
HHHCCCCCEEEECCCCCCEEEEECC
>Mature Secondary Structure
MKSRKVFKGLSRTFIAIIVVVIVVIGIAVGVLLGNHPSSNNISTTTTSSNIISTTTLSST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCC
SSSTSTPNSVSIPSSITVEEAATPVSVDPASSYDIAGGEIIQNVYQTLVFYNGTNTSSFI
CCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEE
GVLAENWTVENNGTTYIFHLWPFITFSNGNPLNATDVWFSIYRTMLINLGISIYTSQALA
EEEECCEEEECCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCEEE
VNNGLGFVGKLPNGKYGTIMLPNGILQALESAGYNFSSNKTIAMEQAAYDLAYILSHFNV
EECCCCEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
SNTTIQKVMSYPHQAVVVIDPYTVEFNLDYPYSAFLAALSTSTGAIVNPVFVDENGGVQI
CHHHHHHHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEE
DTSNTYLSTHALGSGPYILETPIGGSYVVLNASPNYWASKVPTKDLNPMLETPKIKTIII
ECCCCEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
DYQTNEAVRILDLQQEKAQISQIDVIDLQELIGSSGVQQLQNLVNGKTFPTAYTSGNVTI
EECCCCEEEEEECCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEEE
YIWGPSAQIDFLAIDAYQYPFNITAVRLAISHAINPVQIQQQVYKGFAINYVGPLDPSLP
EEECCCCCEEEEEEEEEECCCCHHHEEEEHHHCCCHHHHHHHHHCCEEEEEECCCCCCCC
YYNSSIIGYTYNPSLSIQLLEEAGFKLTLPNGTTVNPNGKPFPTITLTYQTGSTALQDEA
CCCCEEEEEEECCCEEEEEEECCCCEEECCCCCEECCCCCCCCEEEEEEECCCCCHHHHH
LLVQQQLAQIGITVQLNPESAVTIVESYLNPPNSSAYPAFQLAGNFPPVLSPIDPAIYLL
HHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHH
SQARLHHGNPAFVDNSTINQLIVEAVRTNNPQELQHIYNEITLLTLAQAQYVWLDDFLAY
HHHHHCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCEEEEHHHHHHH
TVASSSIHGFWYSPGLDGLFYADLY
HHHCCCCCEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA