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The map label for this gene is dgoD [H]

Identifier: 227828603

GI number: 227828603

Start: 2188053

End: 2189312

Strand: Direct

Name: dgoD [H]

Synonym: M1425_2356

Alternate gene names: 227828603

Gene position: 2188053-2189312 (Clockwise)

Preceding gene: 227828602

Following gene: 227828605

Centisome position: 83.87

GC content: 39.68

Gene sequence:

>1260_bases
ATGGAACTAAAAATCACTGATTTCAAAGTTTTCGTAGCTCAAGCCAATTTCGAATGGGCTTTCGTGAGAATCTACTCCAA
GGACTTGTATGGTACTGGTGAGGCTGGTCCAGCACCGGGTTTAAAAGGAATGGAAAGCGAGTTTAGACAATTACTAATTG
GCGAAGATGCGTTTAAAATTAATAGGATAGTTGAGAAACTGAGATATGCCGCACTATACTCCGGGACTACTGTGTATCAT
CTGATCTCAGCCATTAATATAGCATTATACGATATTATTGGAAAATTCCTAAATGTACCAATATACAAATTGCTAGGAGG
GGATAAGACGGAAATACCAGTATATGTTGACGCTCATGGAGGGAAAGGATTAGAAGCGATTAACGCTTTACACTTACCAG
TTAACTTACCTTGGATAAAGGAAGCTGAAGTGGAGACAAGTAGGTTGCTCACAACCAATAATCCAGTTCATGGTAGGTTG
TCAATGGAAAAGTGGAATGAGGACTACTCCCCTGAGGCTTACGCTAAGAGAGCTTTGAAGATGAAGAACGAGGGTTATAA
AGCCATGAAATTTGACCTAGACGTTCCTACACCTTATATAGATTTAAGGAGAGTGAGAAATGGGGATTTATCCCTTAAAG
ACATCGACTACATGGCAGAAATAGTTAAGGCTGTTAGGGAATCCGTTGGCAATGAAGTCGAAATAATGTTCGATTTGCAT
TGGAGATATAATTTAAACACTGCTATTAGGATTTGCAAGTCATTAGAACCATATAGGCTAAGATGGCTAGAGGATCCAAC
ACCTGCAATAATGGCTGTTCCCAATTACGACGAATTGAAGTTGTTGACATCTCAGTGCTCAGTTCCAATAGAAACTGGTG
AGAATCTCTATACAGTATACCAGTTCAAGGACTTACTGAATACTGGAACAAGAGTATGGGCTCCAGATATAGTTAAGGCT
GGCGGGATAACTGAGGGAAGAAGAATAGCTGAATTAGCTGCAATGTACGATATAGAATATTCTCCTCATAATATTGCTTC
ACCAATAGGTACTATGGCTCACGCTCATGTAGGGTCGATTGCAAACACTTTCGGTTTCGTGGAATTTCACGGTCATGATG
TGCCTTTCTGGAATGAGATAGTTAAACCTAAGAGAAGGATTATAGGGGATGGTGTAATTAAGCTTACCGAGGATCCAGGA
TTAGGTGTTGATTTAGATGATGAGGTTATGAGGAAATACTGGCCAGATTATAATTTATAA

Upstream 100 bases:

>100_bases
AGCACCTGCAAAGCAACAACCCCAACCACAACAACCAAATCCATACCTAGGTTAAAAGGTTAAGATATGATTATTTTATT
TAGGTTTTCTTTATTACTTT

Downstream 100 bases:

>100_bases
AATATGTATGAACTTCCTAACTCATAAACCTAGATATAACTTCAAGTTATTTTTGAAAATGTAATTATAAATTACTGATA
AAGTTTTATATAAAGAAAGA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFRQLLIGEDAFKINRIVEKLRYAALYSGTTVYH
LISAINIALYDIIGKFLNVPIYKLLGGDKTEIPVYVDAHGGKGLEAINALHLPVNLPWIKEAEVETSRLLTTNNPVHGRL
SMEKWNEDYSPEAYAKRALKMKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMAEIVKAVRESVGNEVEIMFDLH
WRYNLNTAIRICKSLEPYRLRWLEDPTPAIMAVPNYDELKLLTSQCSVPIETGENLYTVYQFKDLLNTGTRVWAPDIVKA
GGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSIANTFGFVEFHGHDVPFWNEIVKPKRRIIGDGVIKLTEDPG
LGVDLDDEVMRKYWPDYNL

Sequences:

>Translated_419_residues
MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFRQLLIGEDAFKINRIVEKLRYAALYSGTTVYH
LISAINIALYDIIGKFLNVPIYKLLGGDKTEIPVYVDAHGGKGLEAINALHLPVNLPWIKEAEVETSRLLTTNNPVHGRL
SMEKWNEDYSPEAYAKRALKMKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMAEIVKAVRESVGNEVEIMFDLH
WRYNLNTAIRICKSLEPYRLRWLEDPTPAIMAVPNYDELKLLTSQCSVPIETGENLYTVYQFKDLLNTGTRVWAPDIVKA
GGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSIANTFGFVEFHGHDVPFWNEIVKPKRRIIGDGVIKLTEDPG
LGVDLDDEVMRKYWPDYNL
>Mature_419_residues
MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFRQLLIGEDAFKINRIVEKLRYAALYSGTTVYH
LISAINIALYDIIGKFLNVPIYKLLGGDKTEIPVYVDAHGGKGLEAINALHLPVNLPWIKEAEVETSRLLTTNNPVHGRL
SMEKWNEDYSPEAYAKRALKMKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMAEIVKAVRESVGNEVEIMFDLH
WRYNLNTAIRICKSLEPYRLRWLEDPTPAIMAVPNYDELKLLTSQCSVPIETGENLYTVYQFKDLLNTGTRVWAPDIVKA
GGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSIANTFGFVEFHGHDVPFWNEIVKPKRRIIGDGVIKLTEDPG
LGVDLDDEVMRKYWPDYNL

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=428, Percent_Identity=26.6355140186916, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994953, Length=202, Percent_Identity=31.1881188118812, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 47539; Mature: 47539

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFRQLLIGEDAFKI
CCEEEEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
NRIVEKLRYAALYSGTTVYHLISAINIALYDIIGKFLNVPIYKLLGGDKTEIPVYVDAHG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCC
GKGLEAINALHLPVNLPWIKEAEVETSRLLTTNNPVHGRLSMEKWNEDYSPEAYAKRALK
CCCCHHHEEEECCCCCCCCCCCCCCHHEEEECCCCCCEEEEHHHCCCCCCHHHHHHHHHH
MKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMAEIVKAVRESVGNEVEIMFDLH
HCCCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
WRYNLNTAIRICKSLEPYRLRWLEDPTPAIMAVPNYDELKLLTSQCSVPIETGENLYTVY
EEECCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCEECCCCCEEEHH
QFKDLLNTGTRVWAPDIVKAGGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSI
HHHHHHHCCCEECCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHH
ANTFGFVEFHGHDVPFWNEIVKPKRRIIGDGVIKLTEDPGLGVDLDDEVMRKYWPDYNL
HHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFRQLLIGEDAFKI
CCEEEEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
NRIVEKLRYAALYSGTTVYHLISAINIALYDIIGKFLNVPIYKLLGGDKTEIPVYVDAHG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEECCC
GKGLEAINALHLPVNLPWIKEAEVETSRLLTTNNPVHGRLSMEKWNEDYSPEAYAKRALK
CCCCHHHEEEECCCCCCCCCCCCCCHHEEEECCCCCCEEEEHHHCCCCCCHHHHHHHHHH
MKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMAEIVKAVRESVGNEVEIMFDLH
HCCCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
WRYNLNTAIRICKSLEPYRLRWLEDPTPAIMAVPNYDELKLLTSQCSVPIETGENLYTVY
EEECCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCCEECCCCCEEEHH
QFKDLLNTGTRVWAPDIVKAGGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSI
HHHHHHHCCCEECCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHH
ANTFGFVEFHGHDVPFWNEIVKPKRRIIGDGVIKLTEDPGLGVDLDDEVMRKYWPDYNL
HHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA