BacMap

Definition	Sulfolobus islandicus M.14.25 chromosome, complete genome.
Accession	NC_012588
Length	2,608,832

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The map label for this gene is paaF [C]

Identifier: 227827751

GI number: 227827751

Start: 1386338

End: 1387096

Strand: Direct

Name: paaF [C]

Synonym: M1425_1482

Alternate gene names: 227827751

Gene position: 1386338-1387096 (Clockwise)

Preceding gene: 227827749

Following gene: 227827753

Centisome position: 53.14

GC content: 36.63

Gene sequence:

>759_bases
ATGGAGACAATAATCGTAAGAGAAGAACCCCCAATAGAGTGGATAATATTAAATAGACCAGATAAGCTAAATTCAATTAA
TGTAAAGATGGTAGAGGAAATTGGAAGTGCACTAAGGCAAAATATAAATAATGATAAAATTAAGGTTATCATATTTACGG
GTTCTGGAAAGGCTTTTTCAGCTGGGGCTGATATCTCTCAGTTTAAAGAATTGGACCCAGTTAAAGCGTGGGAATTTGCT
ACAAAGGGAAGAGAATTAATGGACTATATAGAGAGGTATCCTAAGCCTACTATAGCCATGATAAATGGATATGCATTAGG
CGGAGGTTTAGAACTGGCATTAAGTTGTGATCTTAGAATTGCTTCCCAAAATGCGGAACTAGGATTGCCGGAAATCAATT
TAGGAATCTATCCAGGATTCGGTGGTACTCAGAGATTAGTTAGGCTAATTGGAAAAGGGAGGGCATTAGAAATTATCATG
TTAGGTGATAGAATAAAAGCGGATTATGCGGAGAGAATTGGTCTTGTAAATAGAGTCGTAGAATCTTCATCACTTGAAAA
AGAAGTAAGGGAGTTAGCTTTAAAACTTGCAGAAAAACCACAAACGGCTATAAGATTAATTAAGACTGTAGTAAATTACG
GTAATGATGGCCCAATACTTAGTGGTTTAACGTTAGAAAGCTTAGGGTGGGGTATTGCGTTCGCTACAGAGGAAGAGAAG
AAAAGAGTTGATGAGTTTCTTTCAAAGAGGTCTAAATAA

Upstream 100 bases:

>100_bases
TTGATATTATTGTATCTCTTCTAGTCACATGTACTATTACTACTTATAGCTTAAAAAGATAAATATTGCAATGCATCATA
TTCTTTGAGGGTTTCTATTA

Downstream 100 bases:

>100_bases
ATACTGTCTCAAATCTTTCCTTAAATGCGTGTCTAGATAACTTTCCTTTTATAGGTAACTTATATCCACAGAATTTACAC
CTCATATCCTCAGTTAGATG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
METIIVREEPPIEWIILNRPDKLNSINVKMVEEIGSALRQNINNDKIKVIIFTGSGKAFSAGADISQFKELDPVKAWEFA
TKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRIASQNAELGLPEINLGIYPGFGGTQRLVRLIGKGRALEIIM
LGDRIKADYAERIGLVNRVVESSSLEKEVRELALKLAEKPQTAIRLIKTVVNYGNDGPILSGLTLESLGWGIAFATEEEK
KRVDEFLSKRSK

Sequences:

>Translated_252_residues
METIIVREEPPIEWIILNRPDKLNSINVKMVEEIGSALRQNINNDKIKVIIFTGSGKAFSAGADISQFKELDPVKAWEFA
TKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRIASQNAELGLPEINLGIYPGFGGTQRLVRLIGKGRALEIIM
LGDRIKADYAERIGLVNRVVESSSLEKEVRELALKLAEKPQTAIRLIKTVVNYGNDGPILSGLTLESLGWGIAFATEEEK
KRVDEFLSKRSK
>Mature_252_residues
METIIVREEPPIEWIILNRPDKLNSINVKMVEEIGSALRQNINNDKIKVIIFTGSGKAFSAGADISQFKELDPVKAWEFA
TKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRIASQNAELGLPEINLGIYPGFGGTQRLVRLIGKGRALEIIM
LGDRIKADYAERIGLVNRVVESSSLEKEVRELALKLAEKPQTAIRLIKTVVNYGNDGPILSGLTLESLGWGIAFATEEEK
KRVDEFLSKRSK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=236, Percent_Identity=36.4406779661017, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=38.860103626943, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=33.7142857142857, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=28.6307053941909, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=32.5581395348837, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=33.8912133891213, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=252, Percent_Identity=27.3809523809524, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI213417737, Length=215, Percent_Identity=28.8372093023256, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI157694516, Length=215, Percent_Identity=28.8372093023256, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI261878539, Length=103, Percent_Identity=46.6019417475728, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=26.9387755102041, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI157694520, Length=174, Percent_Identity=31.0344827586207, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=210, Percent_Identity=31.4285714285714, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI37594471, Length=210, Percent_Identity=31.4285714285714, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI25453481, Length=203, Percent_Identity=28.0788177339901, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538573, Length=203, Percent_Identity=28.0788177339901, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=203, Percent_Identity=28.0788177339901, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538581, Length=203, Percent_Identity=28.0788177339901, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=199, Percent_Identity=25.6281407035176, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=199, Percent_Identity=25.6281407035176, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=199, Percent_Identity=27.1356783919598, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=199, Percent_Identity=27.1356783919598, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=35.0597609561753, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI221142681, Length=206, Percent_Identity=37.8640776699029, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=38.265306122449, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=37.8531073446328, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=32.03125, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788597, Length=242, Percent_Identity=33.0578512396694, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082183, Length=242, Percent_Identity=28.9256198347107, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=36.3636363636364, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=34.9397590361446, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17540714, Length=216, Percent_Identity=36.5740740740741, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=33.1877729257642, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=29.8642533936652, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=162, Percent_Identity=38.8888888888889, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=32.6732673267327, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=207, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=207, Percent_Identity=34.7826086956522, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=191, Percent_Identity=36.6492146596859, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=191, Percent_Identity=27.7486910994764, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25141363, Length=205, Percent_Identity=28.2926829268293, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=203, Percent_Identity=31.0344827586207, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=203, Percent_Identity=31.0344827586207, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=186, Percent_Identity=30.6451612903226, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=25.5905511811024, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320241, Length=222, Percent_Identity=28.3783783783784, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=38.4920634920635, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=38.4920634920635, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24653139, Length=244, Percent_Identity=35.2459016393443, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=34.9753694581281, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922422, Length=207, Percent_Identity=26.0869565217391, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357171, Length=216, Percent_Identity=32.4074074074074, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921000, Length=208, Percent_Identity=34.1346153846154, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24583077, Length=208, Percent_Identity=34.1346153846154, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24583079, Length=208, Percent_Identity=34.1346153846154, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=33.7209302325581, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=33.7209302325581, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=26.2008733624454, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24654903, Length=199, Percent_Identity=27.1356783919598, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583165, Length=136, Percent_Identity=30.1470588235294, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921018, Length=189, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27944; Mature: 27944

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIIVREEPPIEWIILNRPDKLNSINVKMVEEIGSALRQNINNDKIKVIIFTGSGKAFS
CCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEE
AGADISQFKELDPVKAWEFATKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRI
CCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEEEEEE
ASQNAELGLPEINLGIYPGFGGTQRLVRLIGKGRALEIIMLGDRIKADYAERIGLVNRVV
ECCCCCCCCCEECEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
ESSSLEKEVRELALKLAEKPQTAIRLIKTVVNYGNDGPILSGLTLESLGWGIAFATEEEK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCCCHHHCCCCEEEECCHHH
KRVDEFLSKRSK
HHHHHHHHHCCC
>Mature Secondary Structure
METIIVREEPPIEWIILNRPDKLNSINVKMVEEIGSALRQNINNDKIKVIIFTGSGKAFS
CCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEE
AGADISQFKELDPVKAWEFATKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRI
CCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEEEEEE
ASQNAELGLPEINLGIYPGFGGTQRLVRLIGKGRALEIIMLGDRIKADYAERIGLVNRVV
ECCCCCCCCCEECEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
ESSSLEKEVRELALKLAEKPQTAIRLIKTVVNYGNDGPILSGLTLESLGWGIAFATEEEK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECCCCHHHCCCCEEEECCHHH
KRVDEFLSKRSK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA