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The map label for this gene is birA [C]

Identifier: 227827743

GI number: 227827743

Start: 1379537

End: 1380223

Strand: Direct

Name: birA [C]

Synonym: M1425_1474

Alternate gene names: 227827743

Gene position: 1379537-1380223 (Clockwise)

Preceding gene: 227827742

Following gene: 227827746

Centisome position: 52.88

GC content: 34.06

Gene sequence:

>687_bases
ATGTTGGTAATAAGACTCAATAAGGTTACATCTACACAAGATTTCGCAGAGGCTATTAGTAATATGTTATATGAAGATTT
TTTGATAATTGCAGAAGAGCAAACTAAAGCAAGAGGAAGATTAGGGAGAAGTTGGTATTCTCCTAAAGGTGGGCTATGGT
TTACTTATGTTAAAAAGAACTTTAGAACGGAAGTGATTCAGTTATCTGGTTTGAAAGTTGCCGTTGCAGTTCTAGATGTT
TTAAGAAAGTTTGTTGACGCAAAGATAAGATGGCCAAATGATATAGTGGTTCGTGATAAGAAAATTGCAGGCATCTTAAT
TGAGGCCAAGACTTATGAAGACAACATTAACTTGTCTGATCTCTTCATAGGTGTTGGTATAGATGTAGCAGTAAAGGAAT
TTCCAAGCGATGTAAATGCTACTTCTTTGCATTTAGAACTAAGAAAGGAAGTAGAACTAAGTATAGATGAGGTGATAACA
AAAATAGATGAGTATCTAAACGTTGATAATAAAAAAGCGGTAGATATTGTGAATCAGTTTCTTTCGCTCAAGGATAGAGA
CGTAATACTCAAAGGTGAAAATTGGGAAAAGAAGTGTAAAGCGTTATTTGTAGATTATATGGGGAGACTTATAACAGAAT
GTGGAATATATGAAGTTGAAGATGTGCACAGAGTAGAAACTTTATGA

Upstream 100 bases:

>100_bases
CATAGTGTTAGTGAGAGAGGAATAGGACTGGCGATAATGAATAGGACAAGAAAGGCATCTGGCTTCTCTTCAATTTATAG
TATAGAGGATGTGTGGAAAT

Downstream 100 bases:

>100_bases
TATTGAAAGGTCACTTACTCTTAATTTTCCACTAATTATTTTCCCATAATTTTCTACATCCTTTGAAGAACTTACTAGAT
TTTTCAGAAGTTGTTTAATA

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLVIRLNKVTSTQDFAEAISNMLYEDFLIIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFRTEVIQLSGLKVAVAVLDV
LRKFVDAKIRWPNDIVVRDKKIAGILIEAKTYEDNINLSDLFIGVGIDVAVKEFPSDVNATSLHLELRKEVELSIDEVIT
KIDEYLNVDNKKAVDIVNQFLSLKDRDVILKGENWEKKCKALFVDYMGRLITECGIYEVEDVHRVETL

Sequences:

>Translated_228_residues
MLVIRLNKVTSTQDFAEAISNMLYEDFLIIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFRTEVIQLSGLKVAVAVLDV
LRKFVDAKIRWPNDIVVRDKKIAGILIEAKTYEDNINLSDLFIGVGIDVAVKEFPSDVNATSLHLELRKEVELSIDEVIT
KIDEYLNVDNKKAVDIVNQFLSLKDRDVILKGENWEKKCKALFVDYMGRLITECGIYEVEDVHRVETL
>Mature_228_residues
MLVIRLNKVTSTQDFAEAISNMLYEDFLIIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFRTEVIQLSGLKVAVAVLDV
LRKFVDAKIRWPNDIVVRDKKIAGILIEAKTYEDNINLSDLFIGVGIDVAVKEFPSDVNATSLHLELRKEVELSIDEVIT
KIDEYLNVDNKKAVDIVNQFLSLKDRDVILKGENWEKKCKALFVDYMGRLITECGIYEVEDVHRVETL

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=118, Percent_Identity=38.135593220339, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26145; Mature: 26145

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIRLNKVTSTQDFAEAISNMLYEDFLIIAEEQTKARGRLGRSWYSPKGGLWFTYVKKN
CEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCCCCCCEEEEEEHHC
FRTEVIQLSGLKVAVAVLDVLRKFVDAKIRWPNDIVVRDKKIAGILIEAKTYEDNINLSD
HHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEEEEECCCCCCHHH
LFIGVGIDVAVKEFPSDVNATSLHLELRKEVELSIDEVITKIDEYLNVDNKKAVDIVNQF
EEEECCCHHHHHHCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LSLKDRDVILKGENWEKKCKALFVDYMGRLITECGIYEVEDVHRVETL
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MLVIRLNKVTSTQDFAEAISNMLYEDFLIIAEEQTKARGRLGRSWYSPKGGLWFTYVKKN
CEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCCCCCCEEEEEEHHC
FRTEVIQLSGLKVAVAVLDVLRKFVDAKIRWPNDIVVRDKKIAGILIEAKTYEDNINLSD
HHHHEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEEEEECCCCCCHHH
LFIGVGIDVAVKEFPSDVNATSLHLELRKEVELSIDEVITKIDEYLNVDNKKAVDIVNQF
EEEECCCHHHHHHCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
LSLKDRDVILKGENWEKKCKALFVDYMGRLITECGIYEVEDVHRVETL
HCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]