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The map label for this gene is leuB [H]

Identifier: 227827688

GI number: 227827688

Start: 1334936

End: 1335946

Strand: Direct

Name: leuB [H]

Synonym: M1425_1417

Alternate gene names: 227827688

Gene position: 1334936-1335946 (Clockwise)

Preceding gene: 227827687

Following gene: 227827689

Centisome position: 51.17

GC content: 36.7

Gene sequence:

>1011_bases
ATGACTTTTCAAGTAGCCGTAATTCCTGGTGACGGCATAGGACCAGAGTTATACGAAGGAAGTAAAAGAGTTATAGCTAA
GCTAATAGAGAAATACAATCTTGATATTAACTTAATTGAAGTTGAGGCTGGCGATACAGCATTAAATAAATATGGCGAAG
CCTTACCTAAACAAACCTTAAGCGTTATAGAAAAGGCGGATATAATTCTAAAAGGTCCAGTTGGAGAGACTGCAATGGAT
GTTGTAGTTAAATTGAGACAAATGTATGATATGTATGCAAATATTAGACCAGCTAAGTCACTGCCAAATGTTCAGTCAAA
ATATTCTAATGTTGATTTACTAATTGTAAGAGAAAATACAGAAGATTTGTATAAAGGGTTTGAATTTGTTCCGTCTAGTG
GAGTAGCAATAGCTATTAAAGTAACCACTGAGTTTGCCTCTAAAAGAATAGTTAATGTAGCATTAAATTACGCTTTAATT
AGACGTCATAAAGTTACGTGTGTCCATAAGGCTAACGTTATGAGAATTACGGATGGGCTTTTCGCAAGCGTCTGTAGGGA
AGTCTTGAAAGGCAAGGTGAATTTCGATGAGATGTATGTTGATGCAGCTGCCGCCAATTTAGTGCGAGATCCAACTCGGT
TTGACGTAATAGTGACTAGTAACGTTTATGGCGATATTCTAAGTGATGAGGCTAGTCAGATAGCTGGAAGTTTGGGTCTA
GCTCCATCTGCAAATATTGGAGATAGGAAATCTATGTTTGAACCAGTACATGGCGCAGCTTTTGATATTGCAGGAAAAGG
GATAGCTAATCCTACCGCATTTCTATTATCTGTCTCCATGATGCTTAGTAGAATGTATGATGTATCTAAGGAGAATAAAT
ATATATTAGCATCAAAATCGTTAGAGGGTGCAATTATAGAGACCTACAAAAGTGGGAATAAGCTAACGGAGGATGTTGGC
GGTAATGCTAAGTTAAAGGATATGGTGGATGAAATATATAAGTATATATGA

Upstream 100 bases:

>100_bases
CGTCATAAAACGTACGTCCCCTCAAACTTATAATCTCTTAGGGGAGATAGCGAATAAGATAAGATCAAAAGGTTTTAAGC
TAGACTAATTTTCTTTACAT

Downstream 100 bases:

>100_bases
TATATGTGTGATGTAAGGTTGATTGGGTACGAAGTTTAATTTTTATAAACTTGTCAAGTTTAATAGGGTATGGTGAGTTG
AATTATCCACCGTTATTAAG

Product: isopropylmalate/isohomocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTFQVAVIPGDGIGPELYEGSKRVIAKLIEKYNLDINLIEVEAGDTALNKYGEALPKQTLSVIEKADIILKGPVGETAMD
VVVKLRQMYDMYANIRPAKSLPNVQSKYSNVDLLIVRENTEDLYKGFEFVPSSGVAIAIKVTTEFASKRIVNVALNYALI
RRHKVTCVHKANVMRITDGLFASVCREVLKGKVNFDEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYDVSKENKYILASKSLEGAIIETYKSGNKLTEDVG
GNAKLKDMVDEIYKYI

Sequences:

>Translated_336_residues
MTFQVAVIPGDGIGPELYEGSKRVIAKLIEKYNLDINLIEVEAGDTALNKYGEALPKQTLSVIEKADIILKGPVGETAMD
VVVKLRQMYDMYANIRPAKSLPNVQSKYSNVDLLIVRENTEDLYKGFEFVPSSGVAIAIKVTTEFASKRIVNVALNYALI
RRHKVTCVHKANVMRITDGLFASVCREVLKGKVNFDEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYDVSKENKYILASKSLEGAIIETYKSGNKLTEDVG
GNAKLKDMVDEIYKYI
>Mature_335_residues
TFQVAVIPGDGIGPELYEGSKRVIAKLIEKYNLDINLIEVEAGDTALNKYGEALPKQTLSVIEKADIILKGPVGETAMDV
VVKLRQMYDMYANIRPAKSLPNVQSKYSNVDLLIVRENTEDLYKGFEFVPSSGVAIAIKVTTEFASKRIVNVALNYALIR
RHKVTCVHKANVMRITDGLFASVCREVLKGKVNFDEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGLA
PSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYDVSKENKYILASKSLEGAIIETYKSGNKLTEDVGG
NAKLKDMVDEIYKYI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=346, Percent_Identity=41.907514450867, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=34.6938775510204, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI28178816, Length=342, Percent_Identity=33.6257309941521, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI28178821, Length=295, Percent_Identity=35.5932203389831, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI28178838, Length=292, Percent_Identity=37.3287671232877, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI28178819, Length=158, Percent_Identity=40.5063291139241, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI87081683, Length=337, Percent_Identity=32.3442136498516, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=32.033426183844, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787381, Length=359, Percent_Identity=30.3621169916435, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=337, Percent_Identity=37.0919881305638, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=31.4868804664723, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=33.9181286549708, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25144293, Length=347, Percent_Identity=32.2766570605187, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=37.8531073446328, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=37.0689655172414, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=32.6470588235294, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=29.5392953929539, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=41.0557184750733, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=41.0557184750733, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24661184, Length=348, Percent_Identity=37.6436781609195, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=32.1533923303835, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=32.1533923303835, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20130355, Length=335, Percent_Identity=29.8507462686567, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=29.4985250737463, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=29.4985250737463, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=29.4985250737463, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=29.3255131964809, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=29.3768545994065, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550436, Length=172, Percent_Identity=25.5813953488372, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36794; Mature: 36663

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQVAVIPGDGIGPELYEGSKRVIAKLIEKYNLDINLIEVEAGDTALNKYGEALPKQTL
CEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHH
SVIEKADIILKGPVGETAMDVVVKLRQMYDMYANIRPAKSLPNVQSKYSNVDLLIVRENT
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEEECCH
EDLYKGFEFVPSSGVAIAIKVTTEFASKRIVNVALNYALIRRHKVTCVHKANVMRITDGL
HHHHCCHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEHHHH
FASVCREVLKGKVNFDEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYDVSKENKYILASKS
CCCCCCCCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
LEGAIIETYKSGNKLTEDVGGNAKLKDMVDEIYKYI
CCCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
TFQVAVIPGDGIGPELYEGSKRVIAKLIEKYNLDINLIEVEAGDTALNKYGEALPKQTL
EEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHH
SVIEKADIILKGPVGETAMDVVVKLRQMYDMYANIRPAKSLPNVQSKYSNVDLLIVRENT
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEEECCH
EDLYKGFEFVPSSGVAIAIKVTTEFASKRIVNVALNYALIRRHKVTCVHKANVMRITDGL
HHHHCCHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEHHHH
FASVCREVLKGKVNFDEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
APSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYDVSKENKYILASKS
CCCCCCCCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
LEGAIIETYKSGNKLTEDVGGNAKLKDMVDEIYKYI
CCCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726 [H]