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The map label for this gene is lysC [C]

Identifier: 227827613

GI number: 227827613

Start: 1273295

End: 1274635

Strand: Direct

Name: lysC [C]

Synonym: M1425_1341

Alternate gene names: 227827613

Gene position: 1273295-1274635 (Clockwise)

Preceding gene: 227827612

Following gene: 227827614

Centisome position: 48.81

GC content: 35.12

Gene sequence:

>1341_bases
ATGGCTCTAATAGTTAAAATAGGTGGATCAATACAAAAAGATGAAAAAGATTATGAACTTATCGTAAAGAAGATACAAGA
TTTTTCAAAGAAATCTGATAAGATCATAGTAGTAACATCAGCTATTAAAAACGTTACTAACGAGTTAATAAGTGCAACTT
TGAATACTGATAACTCTCCAAATATTGTAACTGAAATCTATGAAAGGCATATAAAACTGTTATCAAAATTGGCAGATGGC
AAGGAATTCGAGAACTCATTTAAGGATATATCTAGGCTGAGTGATGAACTGTTCAGAGTAGCTTGGTCTATAAGAGTATT
AGACGAAGTAACCCCTAGAGTTAGAGACTACATACTTTCATTTGGAGAGAGAATGGCTACCTTACTCCTTTCAGCAATAT
TGAGAAGTAACGGAATTGAAGCTGAAGGTGTGATTACTCCTCCATTTATAACTGATGAAAATTACGGTGAAGCTAACGTA
ATTGAGGATCTATCTAAAAAGGAAATCATGAGTATATTAGAAAATGTGAAAGCTAACGTGATAGTCCTTCCAGGTTTTAT
AGGGAGAACAAAAGAGGGGAGATACACCACTTTGGGAAGGGGTGGTAGTGATTATACTGCAACCTTACTAGGTAAACTAA
TTGGATTAAGAGAAGTTAGACTAGTTACCGAGGTCCCTGGGATAATGACAGGAGATCCTAAAAAATTCGAAAATGCTAAA
ACGATAACTAGACTCTCGTTGGAAGAAGCTATAGAACTCTCACAGTTAGGAGCAAAAAGGTTACATCCTAGAACTTTTGA
TCCAGTCTTTGGAAGTGATATGAAGGTAATAGTTGAGTCATTATATGAATATGGATTTACCATGATTAATGGAGAATGTG
AAAATAATGATGGACTTAAAGGAATATCACTCTTGGATAACGCAAAATTGATAACAGTTGAGAGTACTAAAATCGTTGGA
AAAATAGGATCTGCGGCAAGAATAACAAATGAAGCGAAGGAGGCTGGAGTTAACATAATATCAATTTCTCAACCAGCTAG
TGAGACCACAATACAACTCGTTGTTGATTCACTCTCTGCAAATAGACTGTTATCGAAATTAGAAGAATTAAAGGGAACTT
TGGTAAAGGATATCGAGGTAAATGATGTTAATATCGTAGGTATAGTAGGATGTGGAATAAAGAAGAAGGAGATCTCTACT
AAGGTTCTTTCAATAGCCTCAAGTTATGATCCGTTAGCTATCTCTAGGGGAATATCTAACGTAAGCATGACCTTTATAGT
TAATAAGGAAGAAGGAGAAAAATTAGCAAAAGAGTTGCATAAGGTGATCGTAAGTGGCTGA

Upstream 100 bases:

>100_bases
GGACATTCATTAAAAGATCCCGATAGTATGACCAAAGCTGAAGCTAAGAGAATATTAGTTAATCCTCTACATATGGAAAA
AATTATCCTAGGTGAGATAA

Downstream 100 bases:

>100_bases
TAAGATAAAAGTTTCGTTACTAGGCTCTACCGGAATGGTAGGGCAAAAAATGGTAAGGATGCTTTCAAAACATCCCTATA
TAGAATTAGTGAAAGTTAGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATLNTDNSPNIVTEIYERHIKLLSKLADG
KEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGVITPPFITDENYGEANV
IEDLSKKEIMSILENVKANVIVLPGFIGRTKEGRYTTLGRGGSDYTATLLGKLIGLREVRLVTEVPGIMTGDPKKFENAK
TITRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEYGFTMINGECENNDGLKGISLLDNAKLITVESTKIVG
KIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEELKGTLVKDIEVNDVNIVGIVGCGIKKKEIST
KVLSIASSYDPLAISRGISNVSMTFIVNKEEGEKLAKELHKVIVSG

Sequences:

>Translated_446_residues
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATLNTDNSPNIVTEIYERHIKLLSKLADG
KEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGVITPPFITDENYGEANV
IEDLSKKEIMSILENVKANVIVLPGFIGRTKEGRYTTLGRGGSDYTATLLGKLIGLREVRLVTEVPGIMTGDPKKFENAK
TITRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEYGFTMINGECENNDGLKGISLLDNAKLITVESTKIVG
KIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEELKGTLVKDIEVNDVNIVGIVGCGIKKKEIST
KVLSIASSYDPLAISRGISNVSMTFIVNKEEGEKLAKELHKVIVSG
>Mature_445_residues
ALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATLNTDNSPNIVTEIYERHIKLLSKLADGK
EFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGVITPPFITDENYGEANVI
EDLSKKEIMSILENVKANVIVLPGFIGRTKEGRYTTLGRGGSDYTATLLGKLIGLREVRLVTEVPGIMTGDPKKFENAKT
ITRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEYGFTMINGECENNDGLKGISLLDNAKLITVESTKIVGK
IGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEELKGTLVKDIEVNDVNIVGIVGCGIKKKEISTK
VLSIASSYDPLAISRGISNVSMTFIVNKEEGEKLAKELHKVIVSG

Specific function: Lysine biosynthesis; first step. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=464, Percent_Identity=26.2931034482759, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790455, Length=401, Percent_Identity=28.6783042394015, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790376, Length=447, Percent_Identity=26.3982102908277, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320893, Length=185, Percent_Identity=28.1081081081081, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48906; Mature: 48775

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATLNTDNSP
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
NIVTEIYERHIKLLSKLADGKEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
FGERMATLLLSAILRSNGIEAEGVITPPFITDENYGEANVIEDLSKKEIMSILENVKANV
HHHHHHHHHHHHHHHCCCCCCCCEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
IVLPGFIGRTKEGRYTTLGRGGSDYTATLLGKLIGLREVRLVTEVPGIMTGDPKKFENAK
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
TITRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEYGFTMINGECENNDGLK
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECEECCCCCCC
GISLLDNAKLITVESTKIVGKIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSA
CEEEECCCEEEEEECHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NRLLSKLEELKGTLVKDIEVNDVNIVGIVGCGIKKKEISTKVLSIASSYDPLAISRGISN
HHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCC
VSMTFIVNKEEGEKLAKELHKVIVSG
EEEEEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATLNTDNSP
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
NIVTEIYERHIKLLSKLADGKEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
FGERMATLLLSAILRSNGIEAEGVITPPFITDENYGEANVIEDLSKKEIMSILENVKANV
HHHHHHHHHHHHHHHCCCCCCCCEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
IVLPGFIGRTKEGRYTTLGRGGSDYTATLLGKLIGLREVRLVTEVPGIMTGDPKKFENAK
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
TITRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEYGFTMINGECENNDGLK
HHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECEECCCCCCC
GISLLDNAKLITVESTKIVGKIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSA
CEEEECCCEEEEEECHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NRLLSKLEELKGTLVKDIEVNDVNIVGIVGCGIKKKEISTKVLSIASSYDPLAISRGISN
HHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCC
VSMTFIVNKEEGEKLAKELHKVIVSG
EEEEEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]