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The map label for this gene is eno

Identifier: 227827580

GI number: 227827580

Start: 1243069

End: 1244328

Strand: Direct

Name: eno

Synonym: M1425_1308

Alternate gene names: 227827580

Gene position: 1243069-1244328 (Clockwise)

Preceding gene: 227827577

Following gene: 227827582

Centisome position: 47.65

GC content: 34.92

Gene sequence:

>1260_bases
ATGATTAACCGTTTTTCCATAGAGAAGGTTAAGGGATTAGAAATCATAGATTCTAGGGGCAATCCCACCATAAGAGTTTT
CGTAAGAACTAATGACGGTGTTGAATCCTTTGGGGACGCGCCAGCAGGAGCTTCTAAAGGAACAAGAGAGGCAATCGAAG
TCAGGGATGAAAATGGGCTTACTGTGAAGAGAGCCGTGGATATTGCAAATTACATAATAGATCCTGCGTTACACGGAATC
GATGTAAGAGAACAAGGTATAATCGACAAAATACTAATAGATATAGACTCCACTGAGAATAAGTCTAAATTAGGAGGAAA
CACAATAATTGCAACATCCATAGCTGCATTAAAAACAGCTTCTAAGGCCTTGGGCCTAGAGGTTTTTAAATACATAGCTG
GGCCTCGGTTACCTAAAATCCCAATACCTTTACTCAATATAATAAATGGCGGCTTACATGCTGGAAATAAGCTAAAAATA
CAAGAATTTATCGTATTACCAATTAAGTTTAATACTTTTAAAGAAGCTTTTTTCGCTGCCATAGAAGTATATAGAAATCT
AAAAGGGCTAATATCAGAGAGATATGGTAAAATTTACACAGCAGTTGGAGATGAAGGAGGATTCTCTCCACCTTTAGAAG
AGACTAGAGAGGCCTTAGATCTGATATATACTTCAATAAATAACGCAGGCTATCAAGGAAAAATATATATGGGAATGGAC
GCTGCAGCAAGTGATTTCTACGATCCTAAGAAAGAGAAATATATAATAGATGGCAAAGAATTGAATCCCAATCAATTACT
TGAATTTTACCTCGACTTAGCTAAAGAATATCCCATAGTGTACTTAGAAGATCCATTCGAAGAGAATTCTTTTGATATGT
TCGGTGAACTACAAAATAAACTAAATTCAACAATCGTCACCGGAGACGACCTATATACTACGAATATAAAATATCTAAAA
ATAGGTATAGAAAAGAGATCAACTAAAGGTGTTATAGTTAAGCCTAATCAAGTCGGTACAATATCTGAAACATTTGAATT
TACTAATCTTGCTAGAAGGAACTCAATAAAGTTAGTAACTAGTCATAGAAGTGGAGAGACGGAAGACAATTTCATAGCAG
AATTTGCAGTGGGAATTGAATCAGATTTCATAAAGACTGGTGCACCAGCAAGAGGAGAAAGAACTAGCAAATATAATAAA
CTGTTAGAAATAGAAAATAAATTTGGATTGGAATATGGAGGAAAATATTTTTATCTTTAA

Upstream 100 bases:

>100_bases
AATCTAGAGAAAATGAGGGAAAGTAAACCATATGCTAAAAACACTATCGAGGCCATGCTAGGAGTTAACATTTAATTATT
GCTAATTCGCTTCAGTAAAT

Downstream 100 bases:

>100_bases
GGCAATAATATCCCTAACACTACAAGGAAAATTGCTATTCCTAGTAAGAACAATATGAACTCCTTAGTTGACATTGATGG
CACTGCTCTAGCTGCCTTCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MINRFSIEKVKGLEIIDSRGNPTIRVFVRTNDGVESFGDAPAGASKGTREAIEVRDENGLTVKRAVDIANYIIDPALHGI
DVREQGIIDKILIDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYIAGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIVLPIKFNTFKEAFFAAIEVYRNLKGLISERYGKIYTAVGDEGGFSPPLEETREALDLIYTSINNAGYQGKIYMGMD
AAASDFYDPKKEKYIIDGKELNPNQLLEFYLDLAKEYPIVYLEDPFEENSFDMFGELQNKLNSTIVTGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSIKLVTSHRSGETEDNFIAEFAVGIESDFIKTGAPARGERTSKYNK
LLEIENKFGLEYGGKYFYL

Sequences:

>Translated_419_residues
MINRFSIEKVKGLEIIDSRGNPTIRVFVRTNDGVESFGDAPAGASKGTREAIEVRDENGLTVKRAVDIANYIIDPALHGI
DVREQGIIDKILIDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYIAGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIVLPIKFNTFKEAFFAAIEVYRNLKGLISERYGKIYTAVGDEGGFSPPLEETREALDLIYTSINNAGYQGKIYMGMD
AAASDFYDPKKEKYIIDGKELNPNQLLEFYLDLAKEYPIVYLEDPFEENSFDMFGELQNKLNSTIVTGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSIKLVTSHRSGETEDNFIAEFAVGIESDFIKTGAPARGERTSKYNK
LLEIENKFGLEYGGKYFYL
>Mature_419_residues
MINRFSIEKVKGLEIIDSRGNPTIRVFVRTNDGVESFGDAPAGASKGTREAIEVRDENGLTVKRAVDIANYIIDPALHGI
DVREQGIIDKILIDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYIAGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIVLPIKFNTFKEAFFAAIEVYRNLKGLISERYGKIYTAVGDEGGFSPPLEETREALDLIYTSINNAGYQGKIYMGMD
AAASDFYDPKKEKYIIDGKELNPNQLLEFYLDLAKEYPIVYLEDPFEENSFDMFGELQNKLNSTIVTGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSIKLVTSHRSGETEDNFIAEFAVGIESDFIKTGAPARGERTSKYNK
LLEIENKFGLEYGGKYFYL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=42.4942263279446, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=42.4942263279446, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI301897477, Length=424, Percent_Identity=42.4528301886792, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI301897469, Length=424, Percent_Identity=42.4528301886792, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI301897479, Length=417, Percent_Identity=39.3285371702638, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.8928571428571, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.8928571428571, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.8928571428571, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789141, Length=418, Percent_Identity=43.3014354066986, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=44.2660550458716, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=44.4444444444444, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI32563855, Length=206, Percent_Identity=41.747572815534, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=40.5963302752294, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=40.3669724770642, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=40.3669724770642, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=41.4578587699317, Blast_Score=300, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=38.6727688787185, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=41.9724770642202, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=41.9724770642202, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=41.9724770642202, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=41.9724770642202, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=41.9354838709677, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=41.9354838709677, Blast_Score=315, Evalue=3e-86,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SULIA (C3N5G6)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843286.1
- ProteinModelPortal:   C3N5G6
- GeneID:   7812569
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1358
- OMA:   IHARQIY
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46652; Mature: 46652

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 334-334 BINDING 153-153 BINDING 162-162 BINDING 283-283 BINDING 309-309 BINDING 334-334 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRFSIEKVKGLEIIDSRGNPTIRVFVRTNDGVESFGDAPAGASKGTREAIEVRDENGL
CCCCCCCHHHCCEEEEECCCCCEEEEEEEECCCHHHCCCCCCCCCCCCCHHEEEECCCCC
TVKRAVDIANYIIDPALHGIDVREQGIIDKILIDIDSTENKSKLGGNTIIATSIAALKTA
EEHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEEEECCCCCCHHCCCCEEEEHHHHHHHHH
SKALGLEVFKYIAGPRLPKIPIPLLNIINGGLHAGNKLKIQEFIVLPIKFNTFKEAFFAA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHH
IEVYRNLKGLISERYGKIYTAVGDEGGFSPPLEETREALDLIYTSINNAGYQGKIYMGMD
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
AAASDFYDPKKEKYIIDGKELNPNQLLEFYLDLAKEYPIVYLEDPFEENSFDMFGELQNK
CHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
LNSTIVTGDDLYTTNIKYLKIGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSIKLVT
HCCEEEECCCEEECCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEE
SHRSGETEDNFIAEFAVGIESDFIKTGAPARGERTSKYNKLLEIENKFGLEYGGKYFYL
ECCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCEEEEC
>Mature Secondary Structure
MINRFSIEKVKGLEIIDSRGNPTIRVFVRTNDGVESFGDAPAGASKGTREAIEVRDENGL
CCCCCCCHHHCCEEEEECCCCCEEEEEEEECCCHHHCCCCCCCCCCCCCHHEEEECCCCC
TVKRAVDIANYIIDPALHGIDVREQGIIDKILIDIDSTENKSKLGGNTIIATSIAALKTA
EEHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEEEECCCCCCHHCCCCEEEEHHHHHHHHH
SKALGLEVFKYIAGPRLPKIPIPLLNIINGGLHAGNKLKIQEFIVLPIKFNTFKEAFFAA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHH
IEVYRNLKGLISERYGKIYTAVGDEGGFSPPLEETREALDLIYTSINNAGYQGKIYMGMD
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
AAASDFYDPKKEKYIIDGKELNPNQLLEFYLDLAKEYPIVYLEDPFEENSFDMFGELQNK
CHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
LNSTIVTGDDLYTTNIKYLKIGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSIKLVT
HCCEEEECCCEEECCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEE
SHRSGETEDNFIAEFAVGIESDFIKTGAPARGERTSKYNKLLEIENKFGLEYGGKYFYL
ECCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA