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The map label for this gene is gatE [H]

Identifier: 227827563

GI number: 227827563

Start: 1225341

End: 1227242

Strand: Direct

Name: gatE [H]

Synonym: M1425_1290

Alternate gene names: 227827563

Gene position: 1225341-1227242 (Clockwise)

Preceding gene: 227827562

Following gene: 227827564

Centisome position: 46.97

GC content: 34.38

Gene sequence:

>1902_bases
ATGAGTGAGTTAAATTATGAAGAATTAGGATTAAAAGTAGGATTAGAAATTCACCAACAGCTTAACACTTCTCATAAATT
ATTCTGCAACTGTAGTACTAATTTGAAAGAAGATTATAAACTAACCTTAGAGAGATATTTAAGACCTGCATTAAGTGAAT
TAGGGGAAGTCGATGTCGCAGCATTGTTTGAGTGGAAAAAAGGTAAAAAATACGTATACAGAATACCTATTACTACAAGT
TGTCTTGTAGAAGCTGATGAAGAACCTCCACATGCAATAAATGAGGAAGCGTTAAAAATAGCGTTAGCTATCGCTATAGC
GCTAAATAGTAACATAGTAGATGAAATTTATGTCATGAGAAAAATTGTAATAGATGGTTCAAACACCACTGGATTCCAAA
GAACAGCAATAGTAGCACTTGGTGGCATGCTAAAAGATGAGGGAGTTACAATACAAACTATTGCAGTTGAGGAGGACGCT
GCAAGGAAAATAGATGAAAGAACAGATCAAGTAACCTATTCGCTTGATAGATTAGGGATTCCCTTAATAGAAATTTCAAC
TGGACCAGATATAAGGAGCCCAGAACAAGCTGAGAGAGTTGCACTAAAAATTGGTCAATTGCTTAGAATGACAGGTAAAG
TTAAAAGGGGTATAGGCACAATAAGACAAGATCTAAATATCTCTATAAAAGGAGGTACAAAGATAGAAATTAAAGGAGTA
CAAAAACTGGAATTAATACCCGATATAGTTAGATATGAAGCAATGAGACAATTTAACTTACTAAAGATAAAAGAAGAATT
GAATAAAAGAGGAGTGAGTAAAAATCTAATTTTGTCTAATTTTGTTGTAAAAGATCTCACAGAACTTTTTAAAAACACAA
ATAGTAAAATCATTAAAAGCGGAATAGAAAAGGGAGGATTAGTATACGGAATAAGAGCGTATAAGTTAAAGGGAATATTG
GGTTGGGAACTGATACCAAAAAAGAGAAGATTTGGAACAGAAATTGCAGATTACGTTAGAGCACTTGCAGAATTAGGTGG
ACTCTTCCATTCTGATGAGCTACCCAATTACGGCATAACTGAAGAGGAGATAAACAAAGTTAGAGAAGCCCTTAATGCGA
CGACCGAAGATGGTTTCATTCTAATCGTTGGCGAAAGAGAGAGGTTGGATAAAGCTGTAGAAGTAATAAGGGACAGGATA
CTATTAGCGTTTGATGGTATTCCTAAGGAAACTAGAGGAGCGTTAGATGATGGAACAACGAAATTCTTAAGACCACAGCC
AAGCTCAGCTAGAATGTATCCAGAAACTGATATTCCACCTAGAAGAATAGATGAAAAACTGCTTGAGGATGCTAAAAAAT
TTGTTCCGGAATCGCCAGAGTCTAAAATGAAAAGGTATATCACACTGGGTTTAAGTGAGGAACTCGCTAAGGAGATAATT
AGAGATCCCAGACTCGACTTATTTGAAGAATTAGTTAACAAATATTCACCAAAGGTCTCTCCAGTTGTAATAGCGAGTAC
GATTACTAACACATTGAAGTATGTTAAGTCAAAAGGTGGTGATATATCAAAGATAAATGAGGAAGATATAGAGGAGCTAA
TAAAAAGCGTTTATGAGAACAAAATTAGCAAGGATTCGATTTCGGAAATACTCCTAGAATACACTACTAATAAAAATGTG
GAACTAAAGGATGTCATACGTAAATACGAGGCATTACCAACTGAAGAATTAGAAAAGATAATAGATGATGTAATTAGTTC
TAATCTAGACGAGATAAGGAAAAGAAAAGATAAAGCAGTAAACCTAATAATGTCAAAAGTAATGAGCAAAGTTAAAGGAA
GAGCTGAGGGGAAAGTTATATTAGAATTAATAAAGTCAAGACTCAAAAATGTTATGGAGTGA

Upstream 100 bases:

>100_bases
CACGAGCAAGATCTAGAAAAAATACGAAGTTTAATGATATCGAATCTAGTGGGGGAAATAAATCCTCGTCACACTTTGGA
TCTCTTCCCAAGGTGGTCAT

Downstream 100 bases:

>100_bases
AGAATTACCCTCGGACAGATTAATGATGAAGAGAAGGCATTTGTCGGATTTAAATGATGAGCTTGACCACTTATGAAGCT
AGATGATATAACAAAGGTAG

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MSELNYEELGLKVGLEIHQQLNTSHKLFCNCSTNLKEDYKLTLERYLRPALSELGEVDVAALFEWKKGKKYVYRIPITTS
CLVEADEEPPHAINEEALKIALAIAIALNSNIVDEIYVMRKIVIDGSNTTGFQRTAIVALGGMLKDEGVTIQTIAVEEDA
ARKIDERTDQVTYSLDRLGIPLIEISTGPDIRSPEQAERVALKIGQLLRMTGKVKRGIGTIRQDLNISIKGGTKIEIKGV
QKLELIPDIVRYEAMRQFNLLKIKEELNKRGVSKNLILSNFVVKDLTELFKNTNSKIIKSGIEKGGLVYGIRAYKLKGIL
GWELIPKKRRFGTEIADYVRALAELGGLFHSDELPNYGITEEEINKVREALNATTEDGFILIVGERERLDKAVEVIRDRI
LLAFDGIPKETRGALDDGTTKFLRPQPSSARMYPETDIPPRRIDEKLLEDAKKFVPESPESKMKRYITLGLSEELAKEII
RDPRLDLFEELVNKYSPKVSPVVIASTITNTLKYVKSKGGDISKINEEDIEELIKSVYENKISKDSISEILLEYTTNKNV
ELKDVIRKYEALPTEELEKIIDDVISSNLDEIRKRKDKAVNLIMSKVMSKVKGRAEGKVILELIKSRLKNVME

Sequences:

>Translated_633_residues
MSELNYEELGLKVGLEIHQQLNTSHKLFCNCSTNLKEDYKLTLERYLRPALSELGEVDVAALFEWKKGKKYVYRIPITTS
CLVEADEEPPHAINEEALKIALAIAIALNSNIVDEIYVMRKIVIDGSNTTGFQRTAIVALGGMLKDEGVTIQTIAVEEDA
ARKIDERTDQVTYSLDRLGIPLIEISTGPDIRSPEQAERVALKIGQLLRMTGKVKRGIGTIRQDLNISIKGGTKIEIKGV
QKLELIPDIVRYEAMRQFNLLKIKEELNKRGVSKNLILSNFVVKDLTELFKNTNSKIIKSGIEKGGLVYGIRAYKLKGIL
GWELIPKKRRFGTEIADYVRALAELGGLFHSDELPNYGITEEEINKVREALNATTEDGFILIVGERERLDKAVEVIRDRI
LLAFDGIPKETRGALDDGTTKFLRPQPSSARMYPETDIPPRRIDEKLLEDAKKFVPESPESKMKRYITLGLSEELAKEII
RDPRLDLFEELVNKYSPKVSPVVIASTITNTLKYVKSKGGDISKINEEDIEELIKSVYENKISKDSISEILLEYTTNKNV
ELKDVIRKYEALPTEELEKIIDDVISSNLDEIRKRKDKAVNLIMSKVMSKVKGRAEGKVILELIKSRLKNVME
>Mature_632_residues
SELNYEELGLKVGLEIHQQLNTSHKLFCNCSTNLKEDYKLTLERYLRPALSELGEVDVAALFEWKKGKKYVYRIPITTSC
LVEADEEPPHAINEEALKIALAIAIALNSNIVDEIYVMRKIVIDGSNTTGFQRTAIVALGGMLKDEGVTIQTIAVEEDAA
RKIDERTDQVTYSLDRLGIPLIEISTGPDIRSPEQAERVALKIGQLLRMTGKVKRGIGTIRQDLNISIKGGTKIEIKGVQ
KLELIPDIVRYEAMRQFNLLKIKEELNKRGVSKNLILSNFVVKDLTELFKNTNSKIIKSGIEKGGLVYGIRAYKLKGILG
WELIPKKRRFGTEIADYVRALAELGGLFHSDELPNYGITEEEINKVREALNATTEDGFILIVGERERLDKAVEVIRDRIL
LAFDGIPKETRGALDDGTTKFLRPQPSSARMYPETDIPPRRIDEKLLEDAKKFVPESPESKMKRYITLGLSEELAKEIIR
DPRLDLFEELVNKYSPKVSPVVIASTITNTLKYVKSKGGDISKINEEDIEELIKSVYENKISKDSISEILLEYTTNKNVE
LKDVIRKYEALPTEELEKIIDDVISSNLDEIRKRKDKAVNLIMSKVMSKVKGRAEGKVILELIKSRLKNVME

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 71529; Mature: 71398

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNYEELGLKVGLEIHQQLNTSHKLFCNCSTNLKEDYKLTLERYLRPALSELGEVDVA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ALFEWKKGKKYVYRIPITTSCLVEADEEPPHAINEEALKIALAIAIALNSNIVDEIYVMR
HHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
KIVIDGSNTTGFQRTAIVALGGMLKDEGVTIQTIAVEEDAARKIDERTDQVTYSLDRLGI
HHEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
PLIEISTGPDIRSPEQAERVALKIGQLLRMTGKVKRGIGTIRQDLNISIKGGTKIEIKGV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCC
QKLELIPDIVRYEAMRQFNLLKIKEELNKRGVSKNLILSNFVVKDLTELFKNTNSKIIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GIEKGGLVYGIRAYKLKGILGWELIPKKRRFGTEIADYVRALAELGGLFHSDELPNYGIT
HHCCCCEEEEEEHHEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
EEEINKVREALNATTEDGFILIVGERERLDKAVEVIRDRILLAFDGIPKETRGALDDGTT
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCC
KFLRPQPSSARMYPETDIPPRRIDEKLLEDAKKFVPESPESKMKRYITLGLSEELAKEII
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
RDPRLDLFEELVNKYSPKVSPVVIASTITNTLKYVKSKGGDISKINEEDIEELIKSVYEN
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KISKDSISEILLEYTTNKNVELKDVIRKYEALPTEELEKIIDDVISSNLDEIRKRKDKAV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NLIMSKVMSKVKGRAEGKVILELIKSRLKNVME
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELNYEELGLKVGLEIHQQLNTSHKLFCNCSTNLKEDYKLTLERYLRPALSELGEVDVA
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ALFEWKKGKKYVYRIPITTSCLVEADEEPPHAINEEALKIALAIAIALNSNIVDEIYVMR
HHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
KIVIDGSNTTGFQRTAIVALGGMLKDEGVTIQTIAVEEDAARKIDERTDQVTYSLDRLGI
HHEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
PLIEISTGPDIRSPEQAERVALKIGQLLRMTGKVKRGIGTIRQDLNISIKGGTKIEIKGV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCC
QKLELIPDIVRYEAMRQFNLLKIKEELNKRGVSKNLILSNFVVKDLTELFKNTNSKIIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
GIEKGGLVYGIRAYKLKGILGWELIPKKRRFGTEIADYVRALAELGGLFHSDELPNYGIT
HHCCCCEEEEEEHHEECCCCCHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
EEEINKVREALNATTEDGFILIVGERERLDKAVEVIRDRILLAFDGIPKETRGALDDGTT
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCCCHHCCCCCCCCC
KFLRPQPSSARMYPETDIPPRRIDEKLLEDAKKFVPESPESKMKRYITLGLSEELAKEII
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHH
RDPRLDLFEELVNKYSPKVSPVVIASTITNTLKYVKSKGGDISKINEEDIEELIKSVYEN
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KISKDSISEILLEYTTNKNVELKDVIRKYEALPTEELEKIIDDVISSNLDEIRKRKDKAV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NLIMSKVMSKVKGRAEGKVILELIKSRLKNVME
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA