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The map label for this gene is gatD [H]

Identifier: 227827562

GI number: 227827562

Start: 1224011

End: 1225348

Strand: Direct

Name: gatD [H]

Synonym: M1425_1289

Alternate gene names: 227827562

Gene position: 1224011-1225348 (Clockwise)

Preceding gene: 227827561

Following gene: 227827563

Centisome position: 46.92

GC content: 36.55

Gene sequence:

>1338_bases
GTGCAAGAAAATTATAAAGCAAAGGCTTATGATATTTTGAAAAATCTAAATATAGAGGAAGGGGACCTAATAGAGATTAA
GAAAGGGGACCTAAGAATAAGAGGCATATTGTTACCAAGTTACTCAAAAGATGAAAGAATATTCGTAATAAAATTAGATA
ACGGTTACAATATAGGAATATCTATCGATAATATATCTGAGATTAAACTAATCACAAAAAATTCAAGTAAAGCTCAAGAG
AGTGAAAGAAAGGAAGTTAGCCGTAATGGTGCTAAAAGCGAAATAAAGATAATTAGCACAGGAGGTACAATAGTAAGTAA
AGTTGAATACGAAACTGGTGCAGTCAGACCCGCGCTGACCACTGAAGAAATAGTACAATTTTTGCCAGAAATAAACGAAA
TAGCAAAAGTTGATGCTGAGGTACTTTTCTCTATATTAAGCGAAAACATGAAACCTGAATACTGGGTTAAGATAGCTGAA
TCAGTAAAGAAAGCGTTTGATGAAGGAAACACTGGAGTGGTTATTGCTCATGGTACTGATACTATGGCATACACAGCCTC
TGCCTTAGCTTTCTCTCTAAGATCATTACAAGGACCAGTAGTGTTGGTAGGTTCGCAGAGAAGTAGTGATAGACCTAGTA
GTGATTCAGCAATAAATCTCTTATCCGCAGTTACCACAGCTAAATATGCGCCTTTTGGAGAAGTAGTAGTAAACATGCAC
GCTGACTCCTCCGACACTTACGCATTAGTACATAGAGGAGTGAAGGTTAGAAAAATGCACAGTAGTAGAAGAGATGCTTT
TCAGTCAGTAAACGATAAACCTTTAGCCAAAGTCCTTTGGAAAGAAAGGAAACTGGTTATGCTCGACAAGAGTTACATGA
GCAAAAAAGGCGAAACTACACTTGACGCTAAATTTGATAATAGAGCATTTTTACTATACTATTACCCTGGATTAGATAGA
GACTTCTTAGAGCACATTTTAACTAATACTAAAATAAGAGGTCTCATAATAGCTGGTACTGGTTTAGGTCATACTTCATC
AGATTATGTGGAATTATTCAGAAAAGCTACTAAAGATGGCATATTTATAGGAATGACAACACAGTGTTTATTTGGAAGAG
TTAATATGAATGTGTATACTACTGGAAGACAGTTACTCGATGCTGGTGTCACCCCATTGGAAGATATGCTACCAGAAGTT
GCATTAGTTAAACTCATGTGGGTACTTGCGCACGAGCAAGATCTAGAAAAAATACGAAGTTTAATGATATCGAATCTAGT
GGGGGAAATAAATCCTCGTCACACTTTGGATCTCTTCCCAAGGTGGTCATATGAGTGA

Upstream 100 bases:

>100_bases
AGTAAGGAATAGAAAGGAATATGAAAAGAGAGTAGTAAAAGCTAGGCAACAAGCTCCTGCTAGATAATAAACTATTTTTG
CTATTACCTAATCTTTTTCT

Downstream 100 bases:

>100_bases
GTTAAATTATGAAGAATTAGGATTAAAAGTAGGATTAGAAATTCACCAACAGCTTAACACTTCTCATAAATTATTCTGCA
ACTGTAGTACTAATTTGAAA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQENYKAKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDERIFVIKLDNGYNIGISIDNISEIKLITKNSSKAQE
SERKEVSRNGAKSEIKIISTGGTIVSKVEYETGAVRPALTTEEIVQFLPEINEIAKVDAEVLFSILSENMKPEYWVKIAE
SVKKAFDEGNTGVVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVTTAKYAPFGEVVVNMH
ADSSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLDKSYMSKKGETTLDAKFDNRAFLLYYYPGLDR
DFLEHILTNTKIRGLIIAGTGLGHTSSDYVELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEV
ALVKLMWVLAHEQDLEKIRSLMISNLVGEINPRHTLDLFPRWSYE

Sequences:

>Translated_445_residues
MQENYKAKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDERIFVIKLDNGYNIGISIDNISEIKLITKNSSKAQE
SERKEVSRNGAKSEIKIISTGGTIVSKVEYETGAVRPALTTEEIVQFLPEINEIAKVDAEVLFSILSENMKPEYWVKIAE
SVKKAFDEGNTGVVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVTTAKYAPFGEVVVNMH
ADSSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLDKSYMSKKGETTLDAKFDNRAFLLYYYPGLDR
DFLEHILTNTKIRGLIIAGTGLGHTSSDYVELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEV
ALVKLMWVLAHEQDLEKIRSLMISNLVGEINPRHTLDLFPRWSYE
>Mature_445_residues
MQENYKAKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDERIFVIKLDNGYNIGISIDNISEIKLITKNSSKAQE
SERKEVSRNGAKSEIKIISTGGTIVSKVEYETGAVRPALTTEEIVQFLPEINEIAKVDAEVLFSILSENMKPEYWVKIAE
SVKKAFDEGNTGVVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVTTAKYAPFGEVVVNMH
ADSSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLDKSYMSKKGETTLDAKFDNRAFLLYYYPGLDR
DFLEHILTNTKIRGLIIAGTGLGHTSSDYVELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEV
ALVKLMWVLAHEQDLEKIRSLMISNLVGEINPRHTLDLFPRWSYE

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=373, Percent_Identity=28.9544235924933, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788065, Length=346, Percent_Identity=33.8150289017341, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789327, Length=328, Percent_Identity=27.4390243902439, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=336, Percent_Identity=32.4404761904762, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193207374, Length=336, Percent_Identity=32.4404761904762, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17558278, Length=336, Percent_Identity=32.4404761904762, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17558280, Length=336, Percent_Identity=32.4404761904762, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320527, Length=138, Percent_Identity=34.0579710144928, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=275, Percent_Identity=24.3636363636364, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323187, Length=275, Percent_Identity=24.3636363636364, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323186, Length=275, Percent_Identity=24.3636363636364, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323184, Length=275, Percent_Identity=24.3636363636364, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639609, Length=390, Percent_Identity=28.7179487179487, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24645530, Length=283, Percent_Identity=31.4487632508834, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 49814; Mature: 49814

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENYKAKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDERIFVIKLDNGYNIGI
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEEEEECCCEEEEE
SIDNISEIKLITKNSSKAQESERKEVSRNGAKSEIKIISTGGTIVSKVEYETGAVRPALT
EECCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCC
TEEIVQFLPEINEIAKVDAEVLFSILSENMKPEYWVKIAESVKKAFDEGNTGVVIAHGTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
TMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVTTAKYAPFGEVVVNMH
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
ADSSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLDKSYMSKKGETT
CCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCE
LDAKFDNRAFLLYYYPGLDRDFLEHILTNTKIRGLIIAGTGLGHTSSDYVELFRKATKDG
EEEEECCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
IFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIRS
EEEEEEHHHHEEEECEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMISNLVGEINPRHTLDLFPRWSYE
HHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQENYKAKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDERIFVIKLDNGYNIGI
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCEEEEEEECCCEEEEE
SIDNISEIKLITKNSSKAQESERKEVSRNGAKSEIKIISTGGTIVSKVEYETGAVRPALT
EECCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCC
TEEIVQFLPEINEIAKVDAEVLFSILSENMKPEYWVKIAESVKKAFDEGNTGVVIAHGTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
TMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVTTAKYAPFGEVVVNMH
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
ADSSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLDKSYMSKKGETT
CCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCE
LDAKFDNRAFLLYYYPGLDRDFLEHILTNTKIRGLIIAGTGLGHTSSDYVELFRKATKDG
EEEEECCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
IFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIRS
EEEEEEHHHHEEEECEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMISNLVGEINPRHTLDLFPRWSYE
HHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA