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The map label for this gene is rfk [H]

Identifier: 227827544

GI number: 227827544

Start: 1211037

End: 1211609

Strand: Direct

Name: rfk [H]

Synonym: M1425_1271

Alternate gene names: 227827544

Gene position: 1211037-1211609 (Clockwise)

Preceding gene: 227827543

Following gene: 227827547

Centisome position: 46.42

GC content: 32.98

Gene sequence:

>573_bases
ATGCTCAATTTCTCACAGCAATCAATATCTCGTAAATTAAAAGAATTAGAGGATGATAAACTGATAGTAAGAATAATATC
TAAAGAAGGAGAGATAATAAGATTAACAGAAGAGGGAGAAAGATTTTTGAGTTCATGTTTAAGTAGCTTAAAAGATGCCA
TATTGTCACTTCATAGTCTTGAGATAAAAGGAAATATCGTATCTGGGCTTGGGGAAGGCAAAATATTTCTATCAATGGAT
TACTATAAAAGCCATATAAACAAAGTAATGGGATTTGACCCTTATCCAGGTACGCTAAATATTGTAATATACGATAAACT
ATCTCTAGAAAATAGATTACTCTTAGACTCATCACCTTCTCTAATAGTGCCCGAATATAAGCAAAAGGATAGAGTATTAG
GCGCAGTTAGATTATATCCTGCCTCAATTAATAAACTAACACCGGCTGCAGTAGTAATTCCCTTAAGAACTACACATCCA
AAGAGCGTAATAGAGGTAATTTCTCCATTTCATCTCAGGGAAAAACTGAATTTAAAAGATGGCGATGAGGTAACAATAGA
GGTTTATGTTTAA

Upstream 100 bases:

>100_bases
GGGATCAACTTGAAAAGATGAGTCAAACGCTTGTATATTAGCTAAAATTATAGCCTATTCAAAGGGCAAATCATACGTAA
CGCAGTCAGAGCTAGCTAAG

Downstream 100 bases:

>100_bases
CGTTGTTCTAATTTACAATACCTCTTTTTAATTTCATTTATTATATCCGTTGTACTTGAATGAGGCCATTTCCTTATTCT
CTCGTTTAATCTAATTATTT

Product: hypothetical protein

Products: NA

Alternate protein names: RFK; CTP-dependent riboflavin kinase; CTP:riboflavin 5'-phosphotransferase; Flavokinase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLNFSQQSISRKLKELEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSLEIKGNIVSGLGEGKIFLSMD
YYKSHINKVMGFDPYPGTLNIVIYDKLSLENRLLLDSSPSLIVPEYKQKDRVLGAVRLYPASINKLTPAAVVIPLRTTHP
KSVIEVISPFHLREKLNLKDGDEVTIEVYV

Sequences:

>Translated_190_residues
MLNFSQQSISRKLKELEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSLEIKGNIVSGLGEGKIFLSMD
YYKSHINKVMGFDPYPGTLNIVIYDKLSLENRLLLDSSPSLIVPEYKQKDRVLGAVRLYPASINKLTPAAVVIPLRTTHP
KSVIEVISPFHLREKLNLKDGDEVTIEVYV
>Mature_190_residues
MLNFSQQSISRKLKELEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSLEIKGNIVSGLGEGKIFLSMD
YYKSHINKVMGFDPYPGTLNIVIYDKLSLENRLLLDSSPSLIVPEYKQKDRVLGAVRLYPASINKLTPAAVVIPLRTTHP
KSVIEVISPFHLREKLNLKDGDEVTIEVYV

Specific function: Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) [H]

COG id: COG1339

COG function: function code KH; Transcriptional regulator of a riboflavin/FAD biosynthetic operon

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal riboflavin kinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015865
- InterPro:   IPR002834 [H]

Pfam domain/function: PF01982 CTP-dep_RFKase [H]

EC number: =2.7.1.161 [H]

Molecular weight: Translated: 21410; Mature: 21410

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFSQQSISRKLKELEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSL
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
EIKGNIVSGLGEGKIFLSMDYYKSHINKVMGFDPYPGTLNIVIYDKLSLENRLLLDSSPS
EECCCEEECCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEEEECCCCC
LIVPEYKQKDRVLGAVRLYPASINKLTPAAVVIPLRTTHPKSVIEVISPFHLREKLNLKD
EEECCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCC
GDEVTIEVYV
CCEEEEEEEC
>Mature Secondary Structure
MLNFSQQSISRKLKELEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSL
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
EIKGNIVSGLGEGKIFLSMDYYKSHINKVMGFDPYPGTLNIVIYDKLSLENRLLLDSSPS
EECCCEEECCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEEEECCCCC
LIVPEYKQKDRVLGAVRLYPASINKLTPAAVVIPLRTTHPKSVIEVISPFHLREKLNLKD
EEECCCCCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCC
GDEVTIEVYV
CCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]