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The map label for this gene is pyk [H]

Identifier: 227827515

GI number: 227827515

Start: 1181885

End: 1183234

Strand: Direct

Name: pyk [H]

Synonym: M1425_1242

Alternate gene names: 227827515

Gene position: 1181885-1183234 (Clockwise)

Preceding gene: 227827514

Following gene: 227827517

Centisome position: 45.3

GC content: 36.0

Gene sequence:

>1350_bases
ATGAGAAAAACTAAAATAGTTGCCACTTTAGGTCCTTCCTCAGAGGAAAAAGTAAAGGAGCTTGCTGAATACGTTGATGT
TTTTAGAATAAATTTTGCTCATGGTGATGAAAAATCCCACAAGAAGTATTTTGATCTCATTATGACATACGCACCAGAGT
CTAGCATCTTAGTAGATTTACCAGGGCCTAAATTGAGACTGGGAGAGCTTAAAGAACCAATAGAGGTGAAGAAAGGAGAT
AAAATAATTTTCTCCCAAAAAGATGGAATTCCAGTCGATGATGAGCTATTCTATTCCGCTGTAAAAGAAAACTCATACAT
CTTAATTGCAGATGGAACAATACGTGTAAGGGTCACGTCGAAAAGTAAGGATAAAGTAGAGGGAATTGTTGTAGAGGGTG
GAATTTTATTATCGAGAAAAGGAATAAATATCCCTAACGTCAGTCTAAAATCTGGGATAACAGATAACGATTTAAAACTT
TTGAAAAGAGCGTTGGATCTGGGAGCAGATTATATAGGTCTCTCTTTTGTAATCAGTGAAGATGATGTAAAGAAGGTAAA
GGAATTTGTAAAAGATGAAGCGTGGATTATTGCAAAGATAGAGAAGAGTGAGGCGTTAAAGAACTTGACCAATATTGTCA
AGGAATCAGATGGAATAATGGTAGCTCGAGGCGATTTGGGAGTTGAGACTGGGCTAGAAAATTTGCCTTTAATTCAAAGG
AGAATAGTAAGGATTTCAAGAGTATTTGGCAAACCCGTTATTTTAGCAACTCAAGTACTAACTTCAATGATAAACAGTCC
TATACCTACTAGGGCGGAAATTATAGATATTTCTAACTCAATCATGCAGGGCGTAGACTCTATAATGTTAAGCGATGAAA
CTGCCATAGGTAATTATCCAGTTGAAAGCGTAAGAACTCTTCATAATATTATAAGTAATGTAGAAAAGAGTATAAAGCAC
GGACCAATTGGGCCACTTAATAGTGAAAGTGATGCAATAGCTTTAGCTGCTGTAAACGCAAGTAAAATATCTAAAGCAGA
CGTAATAGTAGTGTATAGTAGATCAGGGAATTCAATATTGCGTGTATCGAGATTAAGGCCTGAGCGTAACATAATAGGAA
TCTCTCCTGATCCTAAACTATCTAAGAAGTTTAAGCTATGTTATGGTGTAATACCTCTAAGTATAAGCAAGAAAATGGAA
TCAATAGACGAGATAATAGACGTTTCCACAAAGCTGATGGAGAAAAAGGTTAAGGACTTAAGATTTAAAAAAATTGTTAT
TGTAGGAGGGGATCCAAAACAAGAGGCAGGGAAGACTAACTTCGTCATAGTCAAGACATTAGAACAGTAG

Upstream 100 bases:

>100_bases
GTGCAGTTAGAGAAAGTAATGAAGTCCTAAGGAAGGCATGTGAAAACGGATTAAAAGAGGCATGTGAAAACATTAAAGAA
ATAGCTAGTAAGATTATGTA

Downstream 100 bases:

>100_bases
AAGGAATGATAGAATTATTGCAAAAATTATAATTATAACAGAAAAGATAGTATTCTCAGTCTTTTTTGGTTTAATAGAAC
CTTTCTCTAAATCCTTTTCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDEKSHKKYFDLIMTYAPESSILVDLPGPKLRLGELKEPIEVKKGD
KIIFSQKDGIPVDDELFYSAVKENSYILIADGTIRVRVTSKSKDKVEGIVVEGGILLSRKGINIPNVSLKSGITDNDLKL
LKRALDLGADYIGLSFVISEDDVKKVKEFVKDEAWIIAKIEKSEALKNLTNIVKESDGIMVARGDLGVETGLENLPLIQR
RIVRISRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSIKH
GPIGPLNSESDAIALAAVNASKISKADVIVVYSRSGNSILRVSRLRPERNIIGISPDPKLSKKFKLCYGVIPLSISKKME
SIDEIIDVSTKLMEKKVKDLRFKKIVIVGGDPKQEAGKTNFVIVKTLEQ

Sequences:

>Translated_449_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDEKSHKKYFDLIMTYAPESSILVDLPGPKLRLGELKEPIEVKKGD
KIIFSQKDGIPVDDELFYSAVKENSYILIADGTIRVRVTSKSKDKVEGIVVEGGILLSRKGINIPNVSLKSGITDNDLKL
LKRALDLGADYIGLSFVISEDDVKKVKEFVKDEAWIIAKIEKSEALKNLTNIVKESDGIMVARGDLGVETGLENLPLIQR
RIVRISRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSIKH
GPIGPLNSESDAIALAAVNASKISKADVIVVYSRSGNSILRVSRLRPERNIIGISPDPKLSKKFKLCYGVIPLSISKKME
SIDEIIDVSTKLMEKKVKDLRFKKIVIVGGDPKQEAGKTNFVIVKTLEQ
>Mature_449_residues
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDEKSHKKYFDLIMTYAPESSILVDLPGPKLRLGELKEPIEVKKGD
KIIFSQKDGIPVDDELFYSAVKENSYILIADGTIRVRVTSKSKDKVEGIVVEGGILLSRKGINIPNVSLKSGITDNDLKL
LKRALDLGADYIGLSFVISEDDVKKVKEFVKDEAWIIAKIEKSEALKNLTNIVKESDGIMVARGDLGVETGLENLPLIQR
RIVRISRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSIKH
GPIGPLNSESDAIALAAVNASKISKADVIVVYSRSGNSILRVSRLRPERNIIGISPDPKLSKKFKLCYGVIPLSISKKME
SIDEIIDVSTKLMEKKVKDLRFKKIVIVGGDPKQEAGKTNFVIVKTLEQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=32.7235772357724, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=32.7235772357724, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI33286418, Length=484, Percent_Identity=33.2644628099174, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI33286422, Length=484, Percent_Identity=32.8512396694215, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI33286420, Length=484, Percent_Identity=32.8512396694215, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=31.5972222222222, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=31.5972222222222, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=28.2700421940928, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=28.2700421940928, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1787965, Length=439, Percent_Identity=31.8906605922551, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=31.9415448851775, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=32.5102880658436, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=30.9426229508197, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=30.9426229508197, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=30.9426229508197, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=30.9426229508197, Blast_Score=181, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319279, Length=430, Percent_Identity=30.4651162790698, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324923, Length=429, Percent_Identity=28.9044289044289, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=32.3828920570265, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=32.3828920570265, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24648966, Length=404, Percent_Identity=34.4059405940594, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24581235, Length=428, Percent_Identity=28.2710280373832, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24646914, Length=257, Percent_Identity=37.7431906614786, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 49529; Mature: 49529

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDEKSHKKYFDLIMTYAPESSILVDL
CCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEC
PGPKLRLGELKEPIEVKKGDKIIFSQKDGIPVDDELFYSAVKENSYILIADGTIRVRVTS
CCCCEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCEEEEEEEC
KSKDKVEGIVVEGGILLSRKGINIPNVSLKSGITDNDLKLLKRALDLGADYIGLSFVISE
CCCCCCCEEEEECCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECC
DDVKKVKEFVKDEAWIIAKIEKSEALKNLTNIVKESDGIMVARGDLGVETGLENLPLIQR
HHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHH
RIVRISRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYP
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
VESVRTLHNIISNVEKSIKHGPIGPLNSESDAIALAAVNASKISKADVIVVYSRSGNSIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHCCCCCEEEEEECCCCCEE
RVSRLRPERNIIGISPDPKLSKKFKLCYGVIPLSISKKMESIDEIIDVSTKLMEKKVKDL
EEEECCCCCCEEECCCCCCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFKKIVIVGGDPKQEAGKTNFVIVKTLEQ
CEEEEEEECCCCHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDEKSHKKYFDLIMTYAPESSILVDL
CCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEC
PGPKLRLGELKEPIEVKKGDKIIFSQKDGIPVDDELFYSAVKENSYILIADGTIRVRVTS
CCCCEECCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCEEEEEEEC
KSKDKVEGIVVEGGILLSRKGINIPNVSLKSGITDNDLKLLKRALDLGADYIGLSFVISE
CCCCCCCEEEEECCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECC
DDVKKVKEFVKDEAWIIAKIEKSEALKNLTNIVKESDGIMVARGDLGVETGLENLPLIQR
HHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHH
RIVRISRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYP
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
VESVRTLHNIISNVEKSIKHGPIGPLNSESDAIALAAVNASKISKADVIVVYSRSGNSIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHCCCCCEEEEEECCCCCEE
RVSRLRPERNIIGISPDPKLSKKFKLCYGVIPLSISKKMESIDEIIDVSTKLMEKKVKDL
EEEECCCCCCEEECCCCCCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RFKKIVIVGGDPKQEAGKTNFVIVKTLEQ
CEEEEEEECCCCHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA