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The map label for this gene is nadB [H]

Identifier: 227827499

GI number: 227827499

Start: 1163441

End: 1164862

Strand: Direct

Name: nadB [H]

Synonym: M1425_1223

Alternate gene names: 227827499

Gene position: 1163441-1164862 (Clockwise)

Preceding gene: 227827489

Following gene: 227827500

Centisome position: 44.6

GC content: 40.37

Gene sequence:

>1422_bases
ATGTTCGAGTATGATCTACATTTTCGGCAGTGGTCTACGGGTCTAAGCGCTGCAATATCCTTGCATAAATCTGGATACAA
GGTAACCGTTATTTCGAAGAAAATTGATGGGGGCTCAAGTTATTGGGCGAAGGGTGGTATTGCTGCAGCTGTTGGCAACG
AAGATTCCCCAGAACTTCACCTGAGCGATACTTTAAAAGTGGGTGATGGGTTATGTGATGTTAAATCTGTAGATTACGTT
ACGAGGGAGATTCGTTATGTTATTAGCACAGTTGAGAAATGGGGTTTCAGATTTGATGAGGGTTTAAGGTTAGAGGGCGG
GCATTCTAAGAGAAGGATATTGCATAAGACTGACGAGACTGGTAGGGAGATAACTAGTTTCCTTTTGAACTTAGCTAAGA
AAGAGGGAATTAATCTCGTTGAAGATAAGTTACTAGCCTTAAAGGTTAAAGATTGTAAGGTTGTAGGATTCGTAACGGAA
AAGGGTGGAAGTTTTGACGCCGAAAAGGTCGTTTTAGCTACAGGTGGTTATGGCTATTTGTTCAAATTTACTTCTAATCC
TAGTACAAACGTGGGAGACGGTATAGCCATAGCCTTTAGGACTGGAGCTTTGGTTTCAGATATGGAGTTTGTTCAATTCC
ACCCTACTGTTACAACGTTTGATGGTAAAGCTTATCTATTGACTGAAACCCTAAGGGGTGAGGGAGCGATTCTGCTGAAC
GAGAAGGGCGAAAGATTTACTTTCAATTATCACAGTAAGGGTGAATTAGCTCCAAGGGATGTTTTAAGTAGGGCTATCTA
TGACCAGTACAAGAAGGGGTATATAGTTTATATTGATCTTTCACCCATTGAAGATTTTGATGGGAAGTTCCCCGTTTTGA
GTAAATACATTAAGAGGTATGGTAAGAAGTTGCAAGTTTTTCCAGGGGCGCATTATACTATTGGAGGGATAAGGATAAAT
GTTCGTGGAGAAACTAATATTAAAGGTTTATATGCAATAGGTGAGGTTTCGGATAGTGGTCTTCACGGTGCGAATAGATT
GGCTAGTAATTCCTTGGCTGAGGATTTAGTGTATGGTGTTAACTTACCAATGTATATTGATAATTGGGAGGGGTTAAGTG
TGGATGACGCTAAGGAAACTGTTGAGGTTAAGTTAAGTGGTGGGAATTCTTTGAGTTTGGATGAGATAGGAGAGTATAAT
TGGAATTATTTAGGGATTGTCAGAAATGGCGAGGGGTTAAGTAAGTTGGTTAAGGTTTACGAGTCTGCTGATTTGAACGA
TAACGCTACACTGGTTAGCCTATTAAGTGCCAAAGGGGCATTGTTAAGAACGGAAAGTAGAGGTACACATTATAGGGAGG
ATTATCCTAATAAGAGTTGGGAAGACGGTAAGAGGATATATTTCGTGGTGAGCAGAAATTGA

Upstream 100 bases:

>100_bases
AATAACTCTTCAACTCTAGTCATTTTCCGAACATGTGTTCGTATAGCAATCTAGTATAAAAACTTTTAGTTAATAAAAAA
CTTTTTATTTGATCGAACAT

Downstream 100 bases:

>100_bases
TTGAGTGGATTTACGTTAAGAGGCTCTGGGATTATTTAGAAGAAGACGTAATGCCAGAGGATATTACTACCAAATTTTTA
GAGGGTATAAAGGCAAGGGG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MFEYDLHFRQWSTGLSAAISLHKSGYKVTVISKKIDGGSSYWAKGGIAAAVGNEDSPELHLSDTLKVGDGLCDVKSVDYV
TREIRYVISTVEKWGFRFDEGLRLEGGHSKRRILHKTDETGREITSFLLNLAKKEGINLVEDKLLALKVKDCKVVGFVTE
KGGSFDAEKVVLATGGYGYLFKFTSNPSTNVGDGIAIAFRTGALVSDMEFVQFHPTVTTFDGKAYLLTETLRGEGAILLN
EKGERFTFNYHSKGELAPRDVLSRAIYDQYKKGYIVYIDLSPIEDFDGKFPVLSKYIKRYGKKLQVFPGAHYTIGGIRIN
VRGETNIKGLYAIGEVSDSGLHGANRLASNSLAEDLVYGVNLPMYIDNWEGLSVDDAKETVEVKLSGGNSLSLDEIGEYN
WNYLGIVRNGEGLSKLVKVYESADLNDNATLVSLLSAKGALLRTESRGTHYREDYPNKSWEDGKRIYFVVSRN

Sequences:

>Translated_473_residues
MFEYDLHFRQWSTGLSAAISLHKSGYKVTVISKKIDGGSSYWAKGGIAAAVGNEDSPELHLSDTLKVGDGLCDVKSVDYV
TREIRYVISTVEKWGFRFDEGLRLEGGHSKRRILHKTDETGREITSFLLNLAKKEGINLVEDKLLALKVKDCKVVGFVTE
KGGSFDAEKVVLATGGYGYLFKFTSNPSTNVGDGIAIAFRTGALVSDMEFVQFHPTVTTFDGKAYLLTETLRGEGAILLN
EKGERFTFNYHSKGELAPRDVLSRAIYDQYKKGYIVYIDLSPIEDFDGKFPVLSKYIKRYGKKLQVFPGAHYTIGGIRIN
VRGETNIKGLYAIGEVSDSGLHGANRLASNSLAEDLVYGVNLPMYIDNWEGLSVDDAKETVEVKLSGGNSLSLDEIGEYN
WNYLGIVRNGEGLSKLVKVYESADLNDNATLVSLLSAKGALLRTESRGTHYREDYPNKSWEDGKRIYFVVSRN
>Mature_473_residues
MFEYDLHFRQWSTGLSAAISLHKSGYKVTVISKKIDGGSSYWAKGGIAAAVGNEDSPELHLSDTLKVGDGLCDVKSVDYV
TREIRYVISTVEKWGFRFDEGLRLEGGHSKRRILHKTDETGREITSFLLNLAKKEGINLVEDKLLALKVKDCKVVGFVTE
KGGSFDAEKVVLATGGYGYLFKFTSNPSTNVGDGIAIAFRTGALVSDMEFVQFHPTVTTFDGKAYLLTETLRGEGAILLN
EKGERFTFNYHSKGELAPRDVLSRAIYDQYKKGYIVYIDLSPIEDFDGKFPVLSKYIKRYGKKLQVFPGAHYTIGGIRIN
VRGETNIKGLYAIGEVSDSGLHGANRLASNSLAEDLVYGVNLPMYIDNWEGLSVDDAKETVEVKLSGGNSLSLDEIGEYN
WNYLGIVRNGEGLSKLVKVYESADLNDNATLVSLLSAKGALLRTESRGTHYREDYPNKSWEDGKRIYFVVSRN

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=530, Percent_Identity=29.4339622641509, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1788928, Length=520, Percent_Identity=34.0384615384615, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1790597, Length=508, Percent_Identity=31.1023622047244, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1786942, Length=534, Percent_Identity=29.9625468164794, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17505833, Length=525, Percent_Identity=30.6666666666667, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17550100, Length=533, Percent_Identity=30.3939962476548, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71986328, Length=458, Percent_Identity=28.82096069869, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=31.4393939393939, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322416, Length=544, Percent_Identity=29.7794117647059, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320788, Length=461, Percent_Identity=31.236442516269, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6322511, Length=426, Percent_Identity=26.9953051643192, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137288, Length=517, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24655642, Length=517, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24655647, Length=517, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24663005, Length=526, Percent_Identity=28.7072243346008, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 52460; Mature: 52460

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEYDLHFRQWSTGLSAAISLHKSGYKVTVISKKIDGGSSYWAKGGIAAAVGNEDSPELH
CCEEEEEHHHHCCCHHHEEEEECCCEEEEEEEEECCCCCCEECCCCEEEEECCCCCCCEE
LSDTLKVGDGLCDVKSVDYVTREIRYVISTVEKWGFRFDEGLRLEGGHSKRRILHKTDET
EECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCCCH
GREITSFLLNLAKKEGINLVEDKLLALKVKDCKVVGFVTEKGGSFDAEKVVLATGGYGYL
HHHHHHHHHHHHHHCCCCHHHCCEEEEEEECEEEEEEEECCCCCCCCCEEEEEECCCEEE
FKFTSNPSTNVGDGIAIAFRTGALVSDMEFVQFHPTVTTFDGKAYLLTETLRGEGAILLN
EEEECCCCCCCCCCEEEEEECCCEECCCEEEEECCEEEEECCCEEEEEEECCCCCEEEEC
EKGERFTFNYHSKGELAPRDVLSRAIYDQYKKGYIVYIDLSPIEDFDGKFPVLSKYIKRY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
GKKLQVFPGAHYTIGGIRINVRGETNIKGLYAIGEVSDSGLHGANRLASNSLAEDLVYGV
CCEEEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
NLPMYIDNWEGLSVDDAKETVEVKLSGGNSLSLDEIGEYNWNYLGIVRNGEGLSKLVKVY
CCCEEEECCCCCCCCCCCCEEEEEECCCCEECHHHHCCCCCEEEEEEECCCHHHHHHHHH
ESADLNDNATLVSLLSAKGALLRTESRGTHYREDYPNKSWEDGKRIYFVVSRN
HCCCCCCCCHHHEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFEYDLHFRQWSTGLSAAISLHKSGYKVTVISKKIDGGSSYWAKGGIAAAVGNEDSPELH
CCEEEEEHHHHCCCHHHEEEEECCCEEEEEEEEECCCCCCEECCCCEEEEECCCCCCCEE
LSDTLKVGDGLCDVKSVDYVTREIRYVISTVEKWGFRFDEGLRLEGGHSKRRILHKTDET
EECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCCCH
GREITSFLLNLAKKEGINLVEDKLLALKVKDCKVVGFVTEKGGSFDAEKVVLATGGYGYL
HHHHHHHHHHHHHHCCCCHHHCCEEEEEEECEEEEEEEECCCCCCCCCEEEEEECCCEEE
FKFTSNPSTNVGDGIAIAFRTGALVSDMEFVQFHPTVTTFDGKAYLLTETLRGEGAILLN
EEEECCCCCCCCCCEEEEEECCCEECCCEEEEECCEEEEECCCEEEEEEECCCCCEEEEC
EKGERFTFNYHSKGELAPRDVLSRAIYDQYKKGYIVYIDLSPIEDFDGKFPVLSKYIKRY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
GKKLQVFPGAHYTIGGIRINVRGETNIKGLYAIGEVSDSGLHGANRLASNSLAEDLVYGV
CCEEEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
NLPMYIDNWEGLSVDDAKETVEVKLSGGNSLSLDEIGEYNWNYLGIVRNGEGLSKLVKVY
CCCEEEECCCCCCCCCCCCEEEEEECCCCEECHHHHCCCCCEEEEEEECCCHHHHHHHHH
ESADLNDNATLVSLLSAKGALLRTESRGTHYREDYPNKSWEDGKRIYFVVSRN
HCCCCCCCCHHHEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]