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The map label for this gene is ydaP [H]

Identifier: 227827485

GI number: 227827485

Start: 1146860

End: 1148509

Strand: Direct

Name: ydaP [H]

Synonym: M1425_1208

Alternate gene names: 227827485

Gene position: 1146860-1148509 (Clockwise)

Preceding gene: 227827484

Following gene: 227827486

Centisome position: 43.96

GC content: 34.73

Gene sequence:

>1650_bases
ATGCCATCAGTTGCAGAAGTAATAATAAGAGTATTAGAAGATAATGGAATTCAAAGAATATATGGAATTCCTGGAGATTC
CATTGACCCTTTAGTTGACGCGATAAGAAAATCTAAAGTAAAATACGTACAAGTAAGACATGAAGAAGGTGCAGCTTTAG
CTGCCTCGGTCGAAGCGAAAATAACTGGTAAGCCTTCAGCATGTATGGGTACTTCTGGTCCTGGATCAATCCATTTATTA
AATGGATTATACGATGCAAAAATGGATCATGCTCCAGTAATAGCGCTAACTGGACAAGTAGAGTCAGATATGATAGGTCA
CGATTATTTTCAAGAAGTTAACCTAACTAAGTTATTTGATGATGTGGCAGTATATAATCAAATTTTAATTAACCCAGAAA
ACGCGGAATATATAATAAGGAGGGCTATAAGAGAGGCTATTTCCAAAAGGGGAGTAGCTCACATAAATTTACCAGTAGAT
ATTCTCAGAAAGTCCTCAGAATATAAGGGTAGCAAGAATACTGAAGTAGGTAAAGTTAAATATTCGATAGATTTTTCTAG
AGCTAAGGAATTAATCAAAGAAAGTGAGAAACCAGTTTTACTAATTGGAGGAGGGACTAGAGGCCTAGGTAAAGAGATAA
ATAGGTTTGCTGAAAAAATAGGAGCACCAATAATATATACATTAAATGGTAAGGGGATTTTACCAGATTTAGATCCTAAA
GTTATGGGCGGAATAGGTCTTTTAGGAACTAAGCCTTCCATAGAGGCGATGGATAAGGCTGATTTATTAATAATGTTAGG
CGCATCATTTCCTTACGTTAATTTTCTAAATAAGAGTGCCAAAGTGATACAGGTTGATATAGATAATTCTAATATAGGTA
AGAGGTTAGATGTTGATCTCTCTTATCCGATTCCAGTTGCTGAGTTCCTAAATATAGATATCGAAGAGAAATCAGATAAA
TACTATGAAGAGTTAAAAGGAAAGAAGGAAGATTGGCTAGATTCTATAAGTAAGCAGGAGAATAGTTTAGATAAACCAAT
GAAACCTCAGAGAGTAGCTTATATAGTTTCCCAGAAGTGCAAGAAAGACGCAGTAATAGTTACTGATACTGGAAATGTAA
CTATGTGGACTGCTAGACACTTTAGAGCTTCAGGAGAGCAAACCTTTATATTTTCTGCTTGGTTAGGTTCAATGGGCATT
GGAGTCCCAGGAAGTGTAGGGGCTTCTTTTGCTGTAGAAAATAAAAGACAAGTTATTTCTTTTGTAGGAGATGGAGGTTT
TACTATGACTATGATGGAAATGATAACTGCTAAGAAATATGATCTTCCAGTTAAAATAATCGTTTATAATAATTCTAAAT
TAGGAATGATAAAATTTGAACAAGAAGTAATGGGGTACCCAGAATGGGGAGTCGATTTATATAACCCAGATTTCACAAAG
ATAGCTGAATCTATTGGATTTAAAGGATTTAGATTAGAAGAGCCAAAAGAGGCTGAGGAAATAATAGAAGATTTTCTAAA
CACTAAAGGACATGCACTTTTAGATGCAATAGTAGATCCAAATGAGAGACCAATGCCACCTAAACTAACTTTTAAGCAAG
CTGGAGAATACGTTCTTTCAATATTTAGAGAGAAATTAGAGGGTATTTAA

Upstream 100 bases:

>100_bases
TTCCAAATATTTTTCTATATTTTGCATTAAGAGAAGTTATAAGCCAGTTGCGGCTATTATAAAAAAGACTATAAAGGAAT
AAGTAAAATATATATATATT

Downstream 100 bases:

>100_bases
ATGATACTTATCTTAGGTGGAGGATTCGCAGGAGTTTCAGCTTATAATCAAAATAAAGAAAATTCCTTAGTAGTCGACAG
AAAGGACTATTTTCTTTTAA

Product: pyruvate dehydrogenase

Products: acetate; CO2; ferrocytochrome b1

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLL
NGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVD
ILRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVDLSYPIPVAEFLNIDIEEKSDK
YYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGI
GVPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGHALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI

Sequences:

>Translated_549_residues
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLL
NGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVD
ILRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVDLSYPIPVAEFLNIDIEEKSDK
YYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGI
GVPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGHALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI
>Mature_548_residues
PSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLLN
GLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDI
LRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPKV
MGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVDLSYPIPVAEFLNIDIEEKSDKY
YEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIG
VPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTKI
AESIGFKGFRLEEPKEAEEIIEDFLNTKGHALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=23.7847222222222, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=33.8709677419355, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=30.9090909090909, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.2685512367491, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786717, Length=521, Percent_Identity=24.9520153550864, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=23.8351254480287, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=475, Percent_Identity=24.2105263157895, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=475, Percent_Identity=24.2105263157895, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=23.4458259325044, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=552, Percent_Identity=28.2608695652174, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=548, Percent_Identity=23.1751824817518, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320123, Length=496, Percent_Identity=22.5806451612903, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320588, Length=551, Percent_Identity=21.0526315789474, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=25.5144032921811, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2

Molecular weight: Translated: 60765; Mature: 60634

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAK
CCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEE
ITGKPSACMGTSGPGSIHLLNGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFD
ECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHH
DVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDILRKSSEYKGSKNTEVGKVK
HHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCHHCCCCCCCEEEEEE
YSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
EEECHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVDL
HHCCEEEECCCCCCHHCCCCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEE
SYPIPVAEFLNIDIEEKSDKYYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKC
CCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
KKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIGVPGSVGASFAVENKRQVIS
CCCCEEEECCCCEEEEEEHHHCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEECCCCEEEE
FVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHH
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGHALLDAIVDPNERPMPPKLTFKQAGEYVLS
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCCCHHHHHHHHHH
IFREKLEGI
HHHHHHCCC
>Mature Secondary Structure 
PSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAK
CCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEE
ITGKPSACMGTSGPGSIHLLNGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFD
ECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHH
DVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDILRKSSEYKGSKNTEVGKVK
HHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCHHCCCCCCCEEEEEE
YSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
EEECHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVDL
HHCCEEEECCCCCCHHCCCCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEE
SYPIPVAEFLNIDIEEKSDKYYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKC
CCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
KKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIGVPGSVGASFAVENKRQVIS
CCCCEEEECCCCEEEEEEHHHCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEECCCCEEEE
FVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHH
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGHALLDAIVDPNERPMPPKLTFKQAGEYVLS
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCCCCCHHHHHHHHHH
IFREKLEGI
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: pyruvate; ferricytochrome b1; H2O

Specific reaction: pyruvate + ferricytochrome b1 + H2O = acetate + CO2 + ferrocytochrome b1

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]