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The map label for this gene is rnz

Identifier: 227827458

GI number: 227827458

Start: 1111976

End: 1112851

Strand: Direct

Name: rnz

Synonym: M1425_1178

Alternate gene names: 227827458

Gene position: 1111976-1112851 (Clockwise)

Preceding gene: 227827457

Following gene: 227827461

Centisome position: 42.62

GC content: 34.93

Gene sequence:

>876_bases
GTGATTCAAATATTCTTTTTAGGGACAGGTGCAGGTTCCCCTTCTAAAAAGAGGAAATTACCAGCATTCTTGGTAAGAAG
AGAAGGCTTAAACATTTTATTGGATTGTGGAGAAGGAACACAATATACATTAATGAATAATAAATTAGGAATAAATTCAA
TAAAAATTATAGGAATAACTCACATGCATGGAGATCATGTATTTGGATTACTAGGAGTAATTGCAAGCATGGGTCTCCTA
GATAGAAAGGAGACACTATACATTCTCGGCCCGAGAGACCTTAAGGATTTCCTCTACACGTCCTTTGAATACTCTAAATT
TAATCCTTCATTCAAAATTGAATTTATAGATAATTACAATGATCAGAATATTACAATAGCTACATTTAAAACATGCCATA
CCGTAGAGTCCCAAGGGTATTTAATTTCAGAAAGGGATAGAGTGAAAATAGACGAAGAAAAACTAGAAAAGGAAAAAATA
AAGGACTGGAGGGTAATGAGAAAACTAAAAGAAGGAAAAACAGTGGAATATAATGGAAAGTTCTTAAAGCCAGAGGATTA
TTTAGTGATAAAGAGGGGATTAAAAGTAGCCTATACTGGTGATACTATACCTTGCCAATCAGTAATAGAGTCCGTTAAAG
GTGTTGATCTCTTAATTCACGATTCAACATTCTTAAATGAGCCTTCAGCTTGCACGTATGGTCATTCAAATGTTGCAGAT
GCCGCTAAAGTCGCTTTAGAAGCCTCGGTGAAGCTATTGGCCTTAACTCACATAAGTCCCAGATATGAGGATGTAACTGA
ACACTTAAAAGTGGCTAGGCGAATTTTCCCTAAATCAATTTTACCAGATGACTTATCTTACATAACTCTTAAGTAG

Upstream 100 bases:

>100_bases
TTATAGACAAATTAAAGGAAAATAGTTTTATTATAAAAGGATTACATTACTTATATTCACATAAGAGTTTAACAAGGGCA
ATTTACGTGGTGCAGAAAAA

Downstream 100 bases:

>100_bases
TCAAACATCTGGTGGAATAGGATTATCCCAAAGAGGTACACCATGAGCCGTCCTTGGAGCTAACCAGTCGACTACTTCTT
TTGGATTTTGATATTCTATA

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIQIFFLGTGAGSPSKKRKLPAFLVRREGLNILLDCGEGTQYTLMNNKLGINSIKIIGITHMHGDHVFGLLGVIASMGLL
DRKETLYILGPRDLKDFLYTSFEYSKFNPSFKIEFIDNYNDQNITIATFKTCHTVESQGYLISERDRVKIDEEKLEKEKI
KDWRVMRKLKEGKTVEYNGKFLKPEDYLVIKRGLKVAYTGDTIPCQSVIESVKGVDLLIHDSTFLNEPSACTYGHSNVAD
AAKVALEASVKLLALTHISPRYEDVTEHLKVARRIFPKSILPDDLSYITLK

Sequences:

>Translated_291_residues
MIQIFFLGTGAGSPSKKRKLPAFLVRREGLNILLDCGEGTQYTLMNNKLGINSIKIIGITHMHGDHVFGLLGVIASMGLL
DRKETLYILGPRDLKDFLYTSFEYSKFNPSFKIEFIDNYNDQNITIATFKTCHTVESQGYLISERDRVKIDEEKLEKEKI
KDWRVMRKLKEGKTVEYNGKFLKPEDYLVIKRGLKVAYTGDTIPCQSVIESVKGVDLLIHDSTFLNEPSACTYGHSNVAD
AAKVALEASVKLLALTHISPRYEDVTEHLKVARRIFPKSILPDDLSYITLK
>Mature_291_residues
MIQIFFLGTGAGSPSKKRKLPAFLVRREGLNILLDCGEGTQYTLMNNKLGINSIKIIGITHMHGDHVFGLLGVIASMGLL
DRKETLYILGPRDLKDFLYTSFEYSKFNPSFKIEFIDNYNDQNITIATFKTCHTVESQGYLISERDRVKIDEEKLEKEKI
KDWRVMRKLKEGKTVEYNGKFLKPEDYLVIKRGLKVAYTGDTIPCQSVIESVKGVDLLIHDSTFLNEPSACTYGHSNVAD
AAKVALEASVKLLALTHISPRYEDVTEHLKVARRIFPKSILPDDLSYITLK

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=326, Percent_Identity=30.6748466257669, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI87082078, Length=302, Percent_Identity=32.4503311258278, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322932, Length=307, Percent_Identity=25.7328990228013, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_SULIA (C3N554)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843174.1
- ProteinModelPortal:   C3N554
- GeneID:   7814397
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1242
- OMA:   HSTSTMA
- ProtClustDB:   CLSK883695
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIFFLGTGAGSPSKKRKLPAFLVRREGLNILLDCGEGTQYTLMNNKLGINSIKIIGIT
CEEEEEEECCCCCCHHHHCCCHHEEECCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEE
HMHGDHVFGLLGVIASMGLLDRKETLYILGPRDLKDFLYTSFEYSKFNPSFKIEFIDNYN
EECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCEECCCCCCEEEEEEECCC
DQNITIATFKTCHTVESQGYLISERDRVKIDEEKLEKEKIKDWRVMRKLKEGKTVEYNGK
CCEEEEEEEEEEEEECCCCCEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCEEEECCE
FLKPEDYLVIKRGLKVAYTGDTIPCQSVIESVKGVDLLIHDSTFLNEPSACTYGHSNVAD
EECCCCEEEEECCEEEEEECCCCCHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCHHH
AAKVALEASVKLLALTHISPRYEDVTEHLKVARRIFPKSILPDDLSYITLK
HHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MIQIFFLGTGAGSPSKKRKLPAFLVRREGLNILLDCGEGTQYTLMNNKLGINSIKIIGIT
CEEEEEEECCCCCCHHHHCCCHHEEECCCCEEEEECCCCCEEEEECCCCCCCEEEEEEEE
HMHGDHVFGLLGVIASMGLLDRKETLYILGPRDLKDFLYTSFEYSKFNPSFKIEFIDNYN
EECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCCEECCCCCCEEEEEEECCC
DQNITIATFKTCHTVESQGYLISERDRVKIDEEKLEKEKIKDWRVMRKLKEGKTVEYNGK
CCEEEEEEEEEEEEECCCCCEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCEEEECCE
FLKPEDYLVIKRGLKVAYTGDTIPCQSVIESVKGVDLLIHDSTFLNEPSACTYGHSNVAD
EECCCCEEEEECCEEEEEECCCCCHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCHHH
AAKVALEASVKLLALTHISPRYEDVTEHLKVARRIFPKSILPDDLSYITLK
HHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA