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The map label for this gene is prs [H]

Identifier: 227827456

GI number: 227827456

Start: 1110169

End: 1111044

Strand: Direct

Name: prs [H]

Synonym: M1425_1176

Alternate gene names: 227827456

Gene position: 1110169-1111044 (Clockwise)

Preceding gene: 227827447

Following gene: 227827457

Centisome position: 42.55

GC content: 36.87

Gene sequence:

>876_bases
ATGATAATAATAGGTGGAACAGCCACAAATGGAATAGATGAAAGTTTATCAAAAATATTATCCATACCTTTAGTAAAAGT
GGAAAGTAAGATATTCCCAGATGGGGAATCCTACATAAGAGTACCCTCTTCAATTAGAAATGAAGAAGTATTATTGGTAC
AAACAACAGACTATCCCCAAGATAAACATCTAATAGAATTATTCCTAATTGCAGAAACCATCAGAGATCTAGGAGCTAAA
AAGCTAACTGCCGTAGTCCCATACTTAGCGTATAGTAGACAAGATAGAAGATTCAAAGATGGAGAAGCAGTTAGTATAAA
GACGATATTACATATACTCGGTGAAGTAGGAGTTAACGGTTTATTGGTAGTAGAGCCACATAAACCAGAAGAACTGTCAT
ACTTTAAAGGAGAACTTAAAATAGTCCATCCTTATCACCAAATTTCGAGAAAAATAAAGGAAATCGTTGAAGATCCATTC
ATATTGGCACCAGATAGAGGAGCGTTAGATAGAGCCAGAAAAATCGCCGAAGAAATTAGTGTTCCATACTCTTATATCGA
AAAGGAAAGGGATAGGACAACGGGCGAAGTTAGAATAAAAGAGGCACCAAACATAAACCTAAAAGGGAAAGATGTGGTTC
TCATAGATGATATAATTAGTACTGGTGGTACCATAGTACAAGCTACACGTTTAGCCTATTCCTTAGGTGCTAGAAGCGTT
ACAGCTGTAGCAGTCCACCTATTACTAGTGGGAGATGCAAAAGAGAGGCTAAGAGAGGCGGGGGTGAAGACGTTGATAGG
AACCAATACCATTAATATAAAGGACAAAGATATAATAACCATAGACGTTTCACAATCAATAGCACTAAGCTTATGA

Upstream 100 bases:

>100_bases
TCGTCTTCCCCGATCCTGCAGTTCCAAGTATAAAAACGTAGTACATATCTCTTTTCACTCAGCTTTTAATCTTAAAAACT
TCCCCTTAAAGATAATAATA

Downstream 100 bases:

>100_bases
AAACCTACGCTGTATTAAAAGGAAACAATTATTTTTTGTCATTAGCTGAATTGAAAGCTATGCTTAATGACGATAATGCT
GAGATTAACTACTTCACTGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIIIGGTATNGIDESLSKILSIPLVKVESKIFPDGESYIRVPSSIRNEEVLLVQTTDYPQDKHLIELFLIAETIRDLGAK
KLTAVVPYLAYSRQDRRFKDGEAVSIKTILHILGEVGVNGLLVVEPHKPEELSYFKGELKIVHPYHQISRKIKEIVEDPF
ILAPDRGALDRARKIAEEISVPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVLIDDIISTGGTIVQATRLAYSLGARSV
TAVAVHLLLVGDAKERLREAGVKTLIGTNTINIKDKDIITIDVSQSIALSL

Sequences:

>Translated_291_residues
MIIIGGTATNGIDESLSKILSIPLVKVESKIFPDGESYIRVPSSIRNEEVLLVQTTDYPQDKHLIELFLIAETIRDLGAK
KLTAVVPYLAYSRQDRRFKDGEAVSIKTILHILGEVGVNGLLVVEPHKPEELSYFKGELKIVHPYHQISRKIKEIVEDPF
ILAPDRGALDRARKIAEEISVPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVLIDDIISTGGTIVQATRLAYSLGARSV
TAVAVHLLLVGDAKERLREAGVKTLIGTNTINIKDKDIITIDVSQSIALSL
>Mature_291_residues
MIIIGGTATNGIDESLSKILSIPLVKVESKIFPDGESYIRVPSSIRNEEVLLVQTTDYPQDKHLIELFLIAETIRDLGAK
KLTAVVPYLAYSRQDRRFKDGEAVSIKTILHILGEVGVNGLLVVEPHKPEELSYFKGELKIVHPYHQISRKIKEIVEDPF
ILAPDRGALDRARKIAEEISVPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVLIDDIISTGGTIVQATRLAYSLGARSV
TAVAVHLLLVGDAKERLREAGVKTLIGTNTINIKDKDIITIDVSQSIALSL

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=294, Percent_Identity=26.8707482993197, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI4506129, Length=296, Percent_Identity=25.6756756756757, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI84875539, Length=299, Percent_Identity=26.0869565217391, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI28557709, Length=272, Percent_Identity=26.1029411764706, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4506133, Length=310, Percent_Identity=23.5483870967742, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787458, Length=281, Percent_Identity=28.1138790035587, Blast_Score=130, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25149168, Length=294, Percent_Identity=25.5102040816327, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17554704, Length=294, Percent_Identity=25.5102040816327, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17554702, Length=294, Percent_Identity=25.5102040816327, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989924, Length=294, Percent_Identity=25.5102040816327, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17570245, Length=321, Percent_Identity=20.8722741433022, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320946, Length=294, Percent_Identity=26.530612244898, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319403, Length=296, Percent_Identity=26.0135135135135, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321776, Length=289, Percent_Identity=25.2595155709343, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322667, Length=167, Percent_Identity=22.1556886227545, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355239, Length=294, Percent_Identity=27.5510204081633, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45551540, Length=317, Percent_Identity=25.5520504731861, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 32181; Mature: 32181

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIGGTATNGIDESLSKILSIPLVKVESKIFPDGESYIRVPSSIRNEEVLLVQTTDYPQ
CEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCCCCCCEEEEEEECCCCC
DKHLIELFLIAETIRDLGAKKLTAVVPYLAYSRQDRRFKDGEAVSIKTILHILGEVGVNG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
LLVVEPHKPEELSYFKGELKIVHPYHQISRKIKEIVEDPFILAPDRGALDRARKIAEEIS
EEEECCCCCCHHHEECCCEEEECCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
VPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVLIDDIISTGGTIVQATRLAYSLGARSV
CCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCHH
TAVAVHLLLVGDAKERLREAGVKTLIGTNTINIKDKDIITIDVSQSIALSL
HHHHHHHHEECCHHHHHHHCCCCEEECCCEEEECCCEEEEEECCCCEEECC
>Mature Secondary Structure
MIIIGGTATNGIDESLSKILSIPLVKVESKIFPDGESYIRVPSSIRNEEVLLVQTTDYPQ
CEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCCCCCCEEEEEEECCCCC
DKHLIELFLIAETIRDLGAKKLTAVVPYLAYSRQDRRFKDGEAVSIKTILHILGEVGVNG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
LLVVEPHKPEELSYFKGELKIVHPYHQISRKIKEIVEDPFILAPDRGALDRARKIAEEIS
EEEECCCCCCHHHEECCCEEEECCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHC
VPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVLIDDIISTGGTIVQATRLAYSLGARSV
CCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCHH
TAVAVHLLLVGDAKERLREAGVKTLIGTNTINIKDKDIITIDVSQSIALSL
HHHHHHHHEECCHHHHHHHCCCCEEECCCEEEECCCEEEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]