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The map label for this gene is lplA [H]

Identifier: 227827440

GI number: 227827440

Start: 1095345

End: 1096445

Strand: Direct

Name: lplA [H]

Synonym: M1425_1159

Alternate gene names: 227827440

Gene position: 1095345-1096445 (Clockwise)

Preceding gene: 227827439

Following gene: 227827441

Centisome position: 41.99

GC content: 35.24

Gene sequence:

>1101_bases
ATGAGTTGGAGATTTATATCTCTCCCTCCTCAAGACGGCTATCACATGGTAACTTCGTTCGTATCAGTAGCAGATTATGT
AAATAGAGGAGGGAAAAATACATTATTGATTTTTTCAGTTAAGGAGCCCTTTGTAAATGTAGGAGTCCATCAAGAAGTAT
GGCTCGAAGTTGATTTAGAATTTACCAAAAAGATGAAAATTCCAGTAGTTAGGCGTGATTTAGGAGGAGGGACAGTAGTA
ATAACAGCAGGAGAACATGATTATTTTATTGTGGTTAGGCAGGAAGATGCACCAAGAAATCCCACTGAACTATATAGGAA
ATTCCTCACGCCCGTAGTAAACGTTTTACGTTCATATGGGTTGAACGCCGATTTAAGGGATCAAGATATAGTAGTCAATG
GAAAGAAAATAAGTGGAAACGGTGCAATGACACGTGGTAATTCAGTTGTATTAGCTGGCAATATTTTGTTAAGCCTAGAT
GTTGATTTAATTAGTAAGTGCATAAAGGTTCCTACAGAAAAGTTTAGGGACAAAATGGCTAAGGATATGTCAGAATGGTT
AACATCATTAGAAAAGGAATTAGGGTATGTTCCACCTAGGGATGAAATTAACAGAAAACTTAAGGAAGCCTTTGAAAAGG
AATTAGGCATCAAATTCGAGGATTCAACATTAACACCAGAGGAAATAGAATTATGGGAGAAACTGGCAAGTGAAAAGGCT
AAAGAGGAATGGATATTTTACAAGGATAATAGACACCCTAATATTCACACAGAAAGATGCGTTAAAATCTCGTCAGCAGT
AGCTCTTTGTCACCTTGACTATAAGGCTAGAAAGTTGTTAAGAATTACCCTTAAAATAGTAAATAAGAAAATTGATGAAA
TATCAATTTCTGGTGACTTTTTCGTCATGTCACCGAATGGATTCATAGAATATTTGGAAGATAGGCTAAAGGGAGTATCA
GCTAATTTGGAGGAGATAAGAGAAGTAATTCTTGACGAATTTAACGAGAAGAAACCAGTGATATTTGGTTTTAATGAAAA
CGATTTAATAGATGCATTAACTGAAATTATGAGAAAACCTGAAATACAAGAGGTTATTTAA

Upstream 100 bases:

>100_bases
AATCCAGAGGAGTTGAAACAACTAGTTAGTGGACAAGCAGCAATACAGAAATTGAAGGAGTTAATAGCCTCAGAAAAGTT
AACATGCAAGAGGCTATAAA

Downstream 100 bases:

>100_bases
ATTTTTACCTTATTTTCTTATTATATTAAATTACTTAAAGTCGGTTAGTAATATTTAATGGTTATATAGTAGTATAAAAG
TAAAGAAAGGCTTTTTTACT

Product: biotin/lipoate A/B protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase subunit 1 [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSWRFISLPPQDGYHMVTSFVSVADYVNRGGKNTLLIFSVKEPFVNVGVHQEVWLEVDLEFTKKMKIPVVRRDLGGGTVV
ITAGEHDYFIVVRQEDAPRNPTELYRKFLTPVVNVLRSYGLNADLRDQDIVVNGKKISGNGAMTRGNSVVLAGNILLSLD
VDLISKCIKVPTEKFRDKMAKDMSEWLTSLEKELGYVPPRDEINRKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKA
KEEWIFYKDNRHPNIHTERCVKISSAVALCHLDYKARKLLRITLKIVNKKIDEISISGDFFVMSPNGFIEYLEDRLKGVS
ANLEEIREVILDEFNEKKPVIFGFNENDLIDALTEIMRKPEIQEVI

Sequences:

>Translated_366_residues
MSWRFISLPPQDGYHMVTSFVSVADYVNRGGKNTLLIFSVKEPFVNVGVHQEVWLEVDLEFTKKMKIPVVRRDLGGGTVV
ITAGEHDYFIVVRQEDAPRNPTELYRKFLTPVVNVLRSYGLNADLRDQDIVVNGKKISGNGAMTRGNSVVLAGNILLSLD
VDLISKCIKVPTEKFRDKMAKDMSEWLTSLEKELGYVPPRDEINRKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKA
KEEWIFYKDNRHPNIHTERCVKISSAVALCHLDYKARKLLRITLKIVNKKIDEISISGDFFVMSPNGFIEYLEDRLKGVS
ANLEEIREVILDEFNEKKPVIFGFNENDLIDALTEIMRKPEIQEVI
>Mature_365_residues
SWRFISLPPQDGYHMVTSFVSVADYVNRGGKNTLLIFSVKEPFVNVGVHQEVWLEVDLEFTKKMKIPVVRRDLGGGTVVI
TAGEHDYFIVVRQEDAPRNPTELYRKFLTPVVNVLRSYGLNADLRDQDIVVNGKKISGNGAMTRGNSVVLAGNILLSLDV
DLISKCIKVPTEKFRDKMAKDMSEWLTSLEKELGYVPPRDEINRKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKAK
EEWIFYKDNRHPNIHTERCVKISSAVALCHLDYKARKLLRITLKIVNKKIDEISISGDFFVMSPNGFIEYLEDRLKGVSA
NLEEIREVILDEFNEKKPVIFGFNENDLIDALTEIMRKPEIQEVI

Specific function: Lipoate-protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. This subunit can alone only catalyze the lipoat

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 41966; Mature: 41835

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWRFISLPPQDGYHMVTSFVSVADYVNRGGKNTLLIFSVKEPFVNVGVHQEVWLEVDLE
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCEEEEEEEEEE
FTKKMKIPVVRRDLGGGTVVITAGEHDYFIVVRQEDAPRNPTELYRKFLTPVVNVLRSYG
HHCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
LNADLRDQDIVVNGKKISGNGAMTRGNSVVLAGNILLSLDVDLISKCIKVPTEKFRDKMA
CCCCCCCCCEEEECCEECCCCCEECCCEEEEECCEEEEECHHHHHHHHHCCHHHHHHHHH
KDMSEWLTSLEKELGYVPPRDEINRKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHH
KEEWIFYKDNRHPNIHTERCVKISSAVALCHLDYKARKLLRITLKIVNKKIDEISISGDF
HHCEEEEECCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHEEEECCCE
FVMSPNGFIEYLEDRLKGVSANLEEIREVILDEFNEKKPVIFGFNENDLIDALTEIMRKP
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCC
EIQEVI
CHHHCC
>Mature Secondary Structure 
SWRFISLPPQDGYHMVTSFVSVADYVNRGGKNTLLIFSVKEPFVNVGVHQEVWLEVDLE
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCEEEEEEEEEE
FTKKMKIPVVRRDLGGGTVVITAGEHDYFIVVRQEDAPRNPTELYRKFLTPVVNVLRSYG
HHCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
LNADLRDQDIVVNGKKISGNGAMTRGNSVVLAGNILLSLDVDLISKCIKVPTEKFRDKMA
CCCCCCCCCEEEECCEECCCCCEECCCEEEEECCEEEEECHHHHHHHHHCCHHHHHHHHH
KDMSEWLTSLEKELGYVPPRDEINRKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHH
KEEWIFYKDNRHPNIHTERCVKISSAVALCHLDYKARKLLRITLKIVNKKIDEISISGDF
HHCEEEEECCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHEEEECCCE
FVMSPNGFIEYLEDRLKGVSANLEEIREVILDEFNEKKPVIFGFNENDLIDALTEIMRKP
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCC
EIQEVI
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11029001 [H]