Click here to switch to the map view.

The map label for this gene is gcvH2 [H]

Identifier: 227827439

GI number: 227827439

Start: 1094897

End: 1095343

Strand: Direct

Name: gcvH2 [H]

Synonym: M1425_1158

Alternate gene names: 227827439

Gene position: 1094897-1095343 (Clockwise)

Preceding gene: 227827436

Following gene: 227827440

Centisome position: 41.97

GC content: 39.37

Gene sequence:

>447_bases
ATGGTAGTTGAATCAAACTGTGAAATACCAGAAAATCTTTACTACTTTATAGAAGGTAAAAACACAGTTTGGGCTAGATT
AGAGGGCTCAGATACAATTGTTGTAGGAATAACTGATCTCGCACAAACAATGGCAGGGAAGATAGTCAAGGTAAGAATAA
AGAAAAAAGGCACTAAAGTGGAGAAAGGAAGACCAGTAGCCACCATGGAAAGTGGCAAGTGGGCTGGACCAGTTCCCGCA
CCAGTAACTGGAGAAGTGGTTGAGGTTAATGCAGAGGCAGAGAAAAGTCCAGTAATTATAAATCAAGATCCTTATGGAAA
AGGATGGTTAGTAAAGATGAAGATGAGTAATCCAGAGGAGTTGAAACAACTAGTTAGTGGACAAGCAGCAATACAGAAAT
TGAAGGAGTTAATAGCCTCAGAAAAGTTAACATGCAAGAGGCTATAA

Upstream 100 bases:

>100_bases
TATCTAAAAAATAAGATTTCGTTATTCTCATTTTTCTGATAACTGCTTTCTAAAGCAAACTTTTTTTAGTTAATTAGACT
TATTATATTACTGACCTAGT

Downstream 100 bases:

>100_bases
AATGAGTTGGAGATTTATATCTCTCCCTCCTCAAGACGGCTATCACATGGTAACTTCGTTCGTATCAGTAGCAGATTATG
TAAATAGAGGAGGGAAAAAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MVVESNCEIPENLYYFIEGKNTVWARLEGSDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPVIINQDPYGKGWLVKMKMSNPEELKQLVSGQAAIQKLKELIASEKLTCKRL

Sequences:

>Translated_148_residues
MVVESNCEIPENLYYFIEGKNTVWARLEGSDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPVIINQDPYGKGWLVKMKMSNPEELKQLVSGQAAIQKLKELIASEKLTCKRL
>Mature_148_residues
MVVESNCEIPENLYYFIEGKNTVWARLEGSDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPVIINQDPYGKGWLVKMKMSNPEELKQLVSGQAAIQKLKELIASEKLTCKRL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=103, Percent_Identity=36.8932038834951, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI89057342, Length=103, Percent_Identity=35.9223300970874, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=38.8429752066116, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551294, Length=92, Percent_Identity=38.0434782608696, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507493, Length=93, Percent_Identity=36.5591397849462, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319272, Length=106, Percent_Identity=35.8490566037736, Blast_Score=71, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17865652, Length=103, Percent_Identity=35.9223300970874, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR002930
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 16225; Mature: 16225

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVESNCEIPENLYYFIEGKNTVWARLEGSDTIVVGITDLAQTMAGKIVKVRIKKKGTKV
CEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCEEEEEEEECCCCCC
EKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPVIINQDPYGKGWLVKMKMSNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLVSGQAAIQKLKELIASEKLTCKRL
HHHHHCCHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MVVESNCEIPENLYYFIEGKNTVWARLEGSDTIVVGITDLAQTMAGKIVKVRIKKKGTKV
CEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCEEEEEEEECCCCCC
EKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPVIINQDPYGKGWLVKMKMSNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLVSGQAAIQKLKELIASEKLTCKRL
HHHHHCCHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]