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The map label for this gene is pqqE [H]

Identifier: 227827328

GI number: 227827328

Start: 990671

End: 991807

Strand: Direct

Name: pqqE [H]

Synonym: M1425_1047

Alternate gene names: 227827328

Gene position: 990671-991807 (Clockwise)

Preceding gene: 227827327

Following gene: 227827329

Centisome position: 37.97

GC content: 36.06

Gene sequence:

>1137_bases
ATGATTCCAATAAGCGTTCTAGTAACTAATGCTGGTACAGTTTCATTTCACATTAAGGGTGAGTATAATAAGGATAGACC
TAGTAAATTCAGCGACGTATTTAGACCAGTGGTAACGTGGAATCTCACGCGGAAATGCAACTTGAAATGTCTTCATTGTT
ATATTAACGCGTCACCAGAAAGTGAGGATGGGTCAACTACAGAAGAGGCGTTAAGGCTAATAGACGAAATGGCTGAAATG
AAAATTCCATTAATTATAATGAGCGGCGGAGAGCCATTAATGAGAAGGGATTTCTTTGAATTAGCATCATATGCTAGGAT
AAAGGGAATAAAATTAGCCTTATCTACAAATGGCACATTGATTACCGAGAGTGTTGCGAAGAAATTAAAAGAACTGGATT
TCTCTTACGTAGGTATAAGTTTAGATAGTTACGATCCAGAATTTCACGATAAATTTAGAGGTCTAAATGGAGCCTTTAAC
ATGACAGTAAAGGGAATTAAAAATGCAATAAACGTTGGACTAAACGTTGGACTTAGATTTACTATTACGGCTAGAAATAT
TCATCAGATAGACGAATACGTGAAATTGGCCTTGGAACTGGGAGTTAAGAGAATAACGTTTTATCATTTATCAGCTAGTG
GGAGGGGTAAGGAATTAAGGGAGTGGATGTATACTCCAGAAGAATATGTTGGTTTCATAAACAAAATGATTGACTATGCA
ATTAAGCTAAGTGGAAAGATAGAGATTGAGACTACCTTGGGACAATTCGATGGAGTTTACATAGCTAAAAAGTTGGCTAG
AGATGAGGAAGAACTTGAAAAGTACATGAAATTTGTTGAAAATAGTGGAGGATGTGGAAGGAAGATGATATCAATATATC
CAAATGGTGACGTTTACCCTTGTCAATTCATTGACTTTTACAAATTAGGTAACGTTAGGGAAAAACCGCTTAAGGAGATA
ATTAAGAATATTCCCGACCTTTTCATAAATACGGATAAGTATTTGAGGGGGAAATATTGTGATAAATGTAAATATAAATT
GGCGTGTAAAGGAGGAGATAGAGCTAGAGCGTATTATTGGAATGGAGATGTTTATGGAGATGACCCATTATGCCCTTTGA
AAACGCTCCACATTTAG

Upstream 100 bases:

>100_bases
AGAGTTAAAAAGATAATTGTGCAAAACAACCCCGTTTAAGAAAATAATTGCTAAAAGATTAGGGATCACTTTTTTATGAC
TAAGATATCTTTTTATTATT

Downstream 100 bases:

>100_bases
TCTTCTGGGAAGTAACTAAAGCCTGCCCATTAACTTGTAAACACTGTAGAGCTAATGCTATAGATAAGCCGTTGCCAAGT
GAGCTTAATACTGAAGAGAG

Product: radical SAM protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPESEDGSTTEEALRLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGKYCDKCKYKLACKGGDRARAYYWNGDVYGDDPLCPLKTLHI

Sequences:

>Translated_378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPESEDGSTTEEALRLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGKYCDKCKYKLACKGGDRARAYYWNGDVYGDDPLCPLKTLHI
>Mature_378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPESEDGSTTEEALRLIDEMAEM
KIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDKFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGKYCDKCKYKLACKGGDRARAYYWNGDVYGDDPLCPLKTLHI

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=342, Percent_Identity=26.0233918128655, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786999, Length=206, Percent_Identity=24.7572815533981, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43082; Mature: 43082

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
SEDGSTTEEALRLIDEMAEMKIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCE
ITESVAKKLKELDFSYVGISLDSYDPEFHDKFRGLNGAFNMTVKGIKNAINVGLNVGLRF
EHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCEECCEEE
TITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLRGKYCDKCKYKLACKGGDRARAYYW
EEEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCCCCCCCEEEEECCCCCEEEEEE
NGDVYGDDPLCPLKTLHI
CCEEECCCCCCCCEECCC
>Mature Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
SEDGSTTEEALRLIDEMAEMKIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCE
ITESVAKKLKELDFSYVGISLDSYDPEFHDKFRGLNGAFNMTVKGIKNAINVGLNVGLRF
EHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCEECCEEE
TITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLRGKYCDKCKYKLACKGGDRARAYYW
EEEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCCCCCCCEEEEECCCCCEEEEEE
NGDVYGDDPLCPLKTLHI
CCEEECCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA