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The map label for this gene is aes [H]

Identifier: 227826693

GI number: 227826693

Start: 247527

End: 248444

Strand: Direct

Name: aes [H]

Synonym: M1425_0285

Alternate gene names: 227826693

Gene position: 247527-248444 (Clockwise)

Preceding gene: 227826692

Following gene: 227826694

Centisome position: 9.49

GC content: 38.89

Gene sequence:

>918_bases
ATGCCCCTAGACCCTCGAATCAAAGAGCTCCTAGAATCAGGTTTTGTCGTACCAATTGGTAAAGCCTCGGTAGATGAGGT
AAGAAAAATATTTAGGCAATTAGCATCAGCCGCGCCTAAAGTTGAAGTTGGAAAAGTAGAAGATATAAAAATACCAGGTA
GTGAAGCCAGTATAAACGCTCGAGTATATTTGCCAAAGGCTAACGGTCCCTATGGAGTTTTAATATATCTTCATGGAGGA
GGCTTTGTTATAGGAGATGTGGAATCTTATGACCCATTGTGTAGAGCTATCACCAATGCATGCAACTGTGTCGTAGTATC
AGTTGATTATAGGTTAGCTCCAGAATATAAGTTTCCTTCTGCTGTCATCGATTCATTTGATGCTACTAATTGGGTTTATA
ATAATTTGGACAAGTTTGATGGAAAAATGGGTGTCGCTATTGCAGGGGACAGCGCTGGGGGAAATTTGGCAGCAGTTGTA
GCTCTTCTTTCAAAGGGTAAACTTAATTTAAAGTATCAAATTCTAATTTACCCTGCTGTAGGTTTCGATAACGTTTCAAG
ATCCATGATAGAATACTCTGATGGTTTTTTCCTAACTAGAGAACATATAGAGTGGTTCGGTTCTCAATACTTGCGAAGTC
CTGCAGACTTGCTAGACTTTAGGTTTTCTCCAATTCTAGTACAAGATTTAAGCGGATTACCTCCAGCCTTAATAATAACA
GCAGAATATGACCCATTAAGGGATCAAGGAGAAGCTTATGCGAATAGATTACTACAAGCTGGAGTACCGGTTACCAGTGT
TAGGTTCAATAACGTTATACACGGATTCCTTTCATTTTTCCCGTTAATAGAGCAAGGAAGAGATGCTATAGGTTTAATAG
GCTCTGTTTTAAGACGCGCATTTTATGATAAAAGTTAA

Upstream 100 bases:

>100_bases
CTTTACCATAAGGTACTAAAATTAAACCATACGATACTAGTTTAGAAATCATGAATGTGATATGTAAAAAGATTTTATAT
TTCGAATTTCAATATAACAT

Downstream 100 bases:

>100_bases
TAATCTTTTTTATTTTAATTTTTCTTTTAAGGCATTATTAAAAACGTTTAAAAAGAGTCAATAACAAATTAAGACTATGA
AAGCTGTTATTCTTCCAGCT

Product: alpha/beta hydrolase fold-3 domain protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPLDPRIKELLESGFVVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEASINARVYLPKANGPYGVLIYLHGG
GFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPSAVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVV
ALLSKGKLNLKYQILIYPAVGFDNVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIIT
AEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRAFYDKS

Sequences:

>Translated_305_residues
MPLDPRIKELLESGFVVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEASINARVYLPKANGPYGVLIYLHGG
GFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPSAVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVV
ALLSKGKLNLKYQILIYPAVGFDNVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIIT
AEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRAFYDKS
>Mature_304_residues
PLDPRIKELLESGFVVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEASINARVYLPKANGPYGVLIYLHGGG
FVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPSAVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVVA
LLSKGKLNLKYQILIYPAVGFDNVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIITA
EYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRAFYDKS

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=314, Percent_Identity=33.4394904458599, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI206597554, Length=291, Percent_Identity=31.6151202749141, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI68051721, Length=307, Percent_Identity=30.6188925081433, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI226423947, Length=315, Percent_Identity=29.8412698412698, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157041239, Length=266, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21328446, Length=92, Percent_Identity=38.0434782608696, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1786682, Length=251, Percent_Identity=28.6852589641434, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=266, Percent_Identity=31.203007518797, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71996133, Length=306, Percent_Identity=27.1241830065359, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=143, Percent_Identity=39.1608391608392, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=131, Percent_Identity=35.1145038167939, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533412, Length=103, Percent_Identity=39.8058252427184, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=103, Percent_Identity=39.8058252427184, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656084, Length=92, Percent_Identity=41.304347826087, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656076, Length=92, Percent_Identity=41.304347826087, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130169, Length=92, Percent_Identity=41.304347826087, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33420; Mature: 33289

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPRIKELLESGFVVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEASINA
CCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCEEE
RVYLPKANGPYGVLIYLHGGGFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPS
EEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHCCCCEEEEEECCEECCCCCCCH
AVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVVALLSKGKLNLKYQILIYPAV
HHHHCCCCCHHHHHCHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEECC
GFDNVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIIT
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCCEEEEE
AEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRA
ECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FYDKS
HCCCC
>Mature Secondary Structure 
PLDPRIKELLESGFVVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEASINA
CCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCEEE
RVYLPKANGPYGVLIYLHGGGFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPS
EEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHCCCCEEEEEECCEECCCCCCCH
AVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVVALLSKGKLNLKYQILIYPAV
HHHHCCCCCHHHHHCHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEECC
GFDNVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIIT
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCCEEEEE
AEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRA
ECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FYDKS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA