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The map label for this gene is mccB [H]

Identifier: 227826575

GI number: 227826575

Start: 145795

End: 146925

Strand: Direct

Name: mccB [H]

Synonym: M1425_0165

Alternate gene names: 227826575

Gene position: 145795-146925 (Clockwise)

Preceding gene: 227826574

Following gene: 227826578

Centisome position: 5.59

GC content: 37.93

Gene sequence:

>1131_bases
TTGAAAGACGCAACTAGAACAGTAAGGGAAGATATAGATGAAGCGACTGGTGCTATTACTACTCCAATATATCAGACAAC
TGCCTATCATTATCCTGAAGGAGAGAAATACAGATATTCTAGGGAGGCAAATCCCACTGTTCTAGAACTCACTAAGAAAA
TCGTAGAACTTGAGAATGCTGAGATGGGTGTTGCGTTTTCTTCGGGGATGGGTGCTATATCCACATCTGCCTTAGCATTA
TTAAAGCCTGGCAATAGTGTTTTGGTTCATCGTGATATGTTTGGTAGGTCTTATCGTTTCTTTACTGATTATCTTAAAAA
CTGGGGTGTTAATGTAGACGCATCAAACCCTGGTAGTGATAATATTATAGAGAAAGCGAAGTCTAAGAGGTACGATGTAA
TATTTGTAGAGAATATTACGAACCCATTATTAAGGGTTGTGGATATTACTGAATTATCCAAGGTAGCAAAAGAAAGAGGA
AGTATATTAGTTGTGGATGCGACTTTTTCCACTCCAATAAATCAAAAGCCGCTGGAGTTAGGTGCTGATATAGTGGTTCA
TAGCGCATCGAAGTTTTTGGCTGGCCATAATGATGTAATAGCAGGATTAGCTGCTGGTTATGGTAAGTACTTAAATGTTA
TAGATCAAATGAGGAGAACATTAGGTACTTCCTTAGATGCCCATGCGGCATATCTGACTTTGAGGGGTATTAAGACTCTT
AAAATAAGGATGGATGTTATTAATAGGAATGCGGAGCAAATAGCTGAATTTTTAGAAGGACATCCTAAAGTTGTAAAGGT
GTATTACCCTGGTCTTAAGTCCCACGTAGATTATGAAATAGCTAGAAAGGTCCTCAAGGGATTTGGAGGTGTTTTAAGTT
TTGAAGTAAATGGAGGTCAAGAAAGTGCATTGAAAGTAATGAAATCACTTAAGTTAATAATTCCTGCACAAACGCTTGGT
GGAGTGAATTCCGTTATATCGCATCCAGCTACCATGTCACATAGGACACTAAGTTTAGAAGAGAGGAAGATAGTTGGGAT
AACAGATAGTCTTCTCAGACTATCTGTTGGAATAGAAGATGTAAATGACTTGATAGAGGATTTAGATAGAGCATTAGGCA
CTTTATACTAG

Upstream 100 bases:

>100_bases
ACCGATGAAAATAAAATTGCAGAAGAGGGTACTAAAACTTGTAATGAATTTAATGTTGAATGTGAAGTTATCAAAGCTAA
AATTGCTGGAGGTGTAGAGG

Downstream 100 bases:

>100_bases
ATTATTGTCCTTATTTCATGTCTTAATTCTTCCTCTAGCATTTCTCTTATCTCTCTCCTATGGACTCTGCTTAAGTGTAA
ATAAAGTCCTTTTTTCGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKDATRTVREDIDEATGAITTPIYQTTAYHYPEGEKYRYSREANPTVLELTKKIVELENAEMGVAFSSGMGAISTSALAL
LKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVDASNPGSDNIIEKAKSKRYDVIFVENITNPLLRVVDITELSKVAKERG
SILVVDATFSTPINQKPLELGADIVVHSASKFLAGHNDVIAGLAAGYGKYLNVIDQMRRTLGTSLDAHAAYLTLRGIKTL
KIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYEIARKVLKGFGGVLSFEVNGGQESALKVMKSLKLIIPAQTLG
GVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIEDVNDLIEDLDRALGTLY

Sequences:

>Translated_376_residues
MKDATRTVREDIDEATGAITTPIYQTTAYHYPEGEKYRYSREANPTVLELTKKIVELENAEMGVAFSSGMGAISTSALAL
LKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVDASNPGSDNIIEKAKSKRYDVIFVENITNPLLRVVDITELSKVAKERG
SILVVDATFSTPINQKPLELGADIVVHSASKFLAGHNDVIAGLAAGYGKYLNVIDQMRRTLGTSLDAHAAYLTLRGIKTL
KIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYEIARKVLKGFGGVLSFEVNGGQESALKVMKSLKLIIPAQTLG
GVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIEDVNDLIEDLDRALGTLY
>Mature_376_residues
MKDATRTVREDIDEATGAITTPIYQTTAYHYPEGEKYRYSREANPTVLELTKKIVELENAEMGVAFSSGMGAISTSALAL
LKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVDASNPGSDNIIEKAKSKRYDVIFVENITNPLLRVVDITELSKVAKERG
SILVVDATFSTPINQKPLELGADIVVHSASKFLAGHNDVIAGLAAGYGKYLNVIDQMRRTLGTSLDAHAAYLTLRGIKTL
KIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYEIARKVLKGFGGVLSFEVNGGQESALKVMKSLKLIIPAQTLG
GVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIEDVNDLIEDLDRALGTLY

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=356, Percent_Identity=34.8314606741573, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI299473759, Length=356, Percent_Identity=31.7415730337079, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI25453487, Length=355, Percent_Identity=31.5492957746479, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=38.8297872340425, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1789383, Length=344, Percent_Identity=28.1976744186047, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=38.7096774193548, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17560056, Length=365, Percent_Identity=35.6164383561644, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=331, Percent_Identity=35.6495468277946, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=37.0466321243523, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=424, Percent_Identity=30.188679245283, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=32.0802005012531, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=393, Percent_Identity=27.9898218829517, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=311, Percent_Identity=28.9389067524116, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922554, Length=363, Percent_Identity=34.9862258953168, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 41343; Mature: 41343

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDATRTVREDIDEATGAITTPIYQTTAYHYPEGEKYRYSREANPTVLELTKKIVELENA
CCCHHHHHHHHHHHHCCCEECCEEECCEEECCCCCCEEECCCCCCHHHHHHHHHHHHCCC
EMGVAFSSGMGAISTSALALLKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVDASNPGSD
CCCEEECCCCCCHHHCEEEEECCCCCEEEEEHHCCCHHHHHHHHHHHCCCEEECCCCCCH
NIIEKAKSKRYDVIFVENITNPLLRVVDITELSKVAKERGSILVVDATFSTPINQKPLEL
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
GADIVVHSASKFLAGHNDVIAGLAAGYGKYLNVIDQMRRTLGTSLDAHAAYLTLRGIKTL
CCHHEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCCEEE
KIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYEIARKVLKGFGGVLSFEVNGGQ
EEEEHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCH
ESALKVMKSLKLIIPAQTLGGVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIED
HHHHHHHHHHHHEECHHHHCCHHHHHCCCCCCCCCEEECCCCEEECHHHHHHHHHCCHHH
VNDLIEDLDRALGTLY
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDATRTVREDIDEATGAITTPIYQTTAYHYPEGEKYRYSREANPTVLELTKKIVELENA
CCCHHHHHHHHHHHHCCCEECCEEECCEEECCCCCCEEECCCCCCHHHHHHHHHHHHCCC
EMGVAFSSGMGAISTSALALLKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVDASNPGSD
CCCEEECCCCCCHHHCEEEEECCCCCEEEEEHHCCCHHHHHHHHHHHCCCEEECCCCCCH
NIIEKAKSKRYDVIFVENITNPLLRVVDITELSKVAKERGSILVVDATFSTPINQKPLEL
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
GADIVVHSASKFLAGHNDVIAGLAAGYGKYLNVIDQMRRTLGTSLDAHAAYLTLRGIKTL
CCHHEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCCEEE
KIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYEIARKVLKGFGGVLSFEVNGGQ
EEEEHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCH
ESALKVMKSLKLIIPAQTLGGVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIED
HHHHHHHHHHHHEECHHHHCCHHHHHCCCCCCCCCEEECCCCEEECHHHHHHHHHCCHHH
VNDLIEDLDRALGTLY
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]