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The map label for this gene is nagD [H]

Identifier: 227826564

GI number: 227826564

Start: 134387

End: 135181

Strand: Direct

Name: nagD [H]

Synonym: M1425_0154

Alternate gene names: 227826564

Gene position: 134387-135181 (Clockwise)

Preceding gene: 227826563

Following gene: 227826565

Centisome position: 5.15

GC content: 36.48

Gene sequence:

>795_bases
ATGTCAACACTTAATGACTATCAATTAATAATAAGTGATGTAGATGGGGTAATAGTAAGAGAAGGGGAACCAATATGGGA
AAATATACAAGCGTTAAGGAATATACAAAATAATGGAGTTAAGGTTATATTTGTAACTAATAATTCCGGTTTTAGTAGGA
TCTTATTATCTAGACAGCTGTCCTATTTAGGCCTTAAGGTTACTCCAGATATGATAATTACAAGTGGCTTGGCTGCTGCA
ATTTACATGAAGGAGAAGCTTAATGTTAAGTCTGTATTCGCAGTAGGCGAAGAGGGGCTTATTGAAGAATTGAAAAATCA
TGGTTTCTTAGTATTCTCTAGTGTGGAATCAGAGAGAAACTTACCAGATGCCGTAGTGATGGGACTGGATAGGTTAAGTA
CGTATGATAAACTATCGTTAGCCATGAGGTGTATAAGCAAGGGATCGAAATTCATAGTAACAAATATGGATAGACTTTGG
CCAGCTAAGGATGGGTTAAAGTTGGGTGCTGGGGCGTTGGCTAGTTCTATAATTTACGCCTTGAGAAGGGATCCAGACTT
TATAGCAGGGAAACCTAACACCTGGATAATAGAAATAGCCATGCGAATTTCAAGTGTTAAGAAGTTAGATAAAATTCTAG
TTATAGGGGATCAGATAGAGACTGATATCCAAATGGGATACAATATAGGTGCTGATACAGCATTAGTTTTAACTGGTATA
TCAACTGTTGACGATGTTGATAGGAGTAGTGTTAAGCCGAAATACGTAGTAAATAGTTTATTAGACCTTTTATGA

Upstream 100 bases:

>100_bases
GAAAACGTACTCTATACTGCAATCGGGCTTTTTGGCTTTGTGAATAAACAAGATGAATTGGAACAAAAAATTAGAGAAAA
GATGGAGGCGTTAATCAAAA

Downstream 100 bases:

>100_bases
AATTGTAGAAAGAATACTTAAAAACTATTATGCAAATATAAACTTGTATGGAAAAGATCGAATACGATGCAGTTGTAATT
GGAGGAGGACTAGCTGGCTT

Product: HAD-superfamily hydrolase, subfamily IIA

Products: 4-nitrophenol; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSTLNDYQLIISDVDGVIVREGEPIWENIQALRNIQNNGVKVIFVTNNSGFSRILLSRQLSYLGLKVTPDMIITSGLAAA
IYMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSVESERNLPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLW
PAKDGLKLGAGALASSIIYALRRDPDFIAGKPNTWIIEIAMRISSVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGI
STVDDVDRSSVKPKYVVNSLLDLL

Sequences:

>Translated_264_residues
MSTLNDYQLIISDVDGVIVREGEPIWENIQALRNIQNNGVKVIFVTNNSGFSRILLSRQLSYLGLKVTPDMIITSGLAAA
IYMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSVESERNLPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLW
PAKDGLKLGAGALASSIIYALRRDPDFIAGKPNTWIIEIAMRISSVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGI
STVDDVDRSSVKPKYVVNSLLDLL
>Mature_263_residues
STLNDYQLIISDVDGVIVREGEPIWENIQALRNIQNNGVKVIFVTNNSGFSRILLSRQLSYLGLKVTPDMIITSGLAAAI
YMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSVESERNLPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLWP
AKDGLKLGAGALASSIIYALRRDPDFIAGKPNTWIIEIAMRISSVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGIS
TVDDVDRSSVKPKYVVNSLLDLL

Specific function: Unknown

COG id: COG0647

COG function: function code G; Predicted sugar phosphatases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. NagD family [H]

Homologues:

Organism=Homo sapiens, GI108796653, Length=294, Percent_Identity=27.5510204081633, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI10092677, Length=277, Percent_Identity=28.158844765343, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI14149777, Length=261, Percent_Identity=25.6704980842912, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786890, Length=254, Percent_Identity=29.1338582677165, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17562458, Length=296, Percent_Identity=26.0135135135135, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560956, Length=296, Percent_Identity=26.0135135135135, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558880, Length=287, Percent_Identity=26.1324041811847, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193210059, Length=273, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319965, Length=246, Percent_Identity=28.8617886178862, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24666141, Length=264, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24656326, Length=260, Percent_Identity=25.7692307692308, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656330, Length=254, Percent_Identity=31.496062992126, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19920940, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22026920, Length=242, Percent_Identity=27.6859504132231, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006357
- InterPro:   IPR006354
- InterPro:   IPR023215 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.41

Molecular weight: Translated: 29054; Mature: 28923

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNDYQLIISDVDGVIVREGEPIWENIQALRNIQNNGVKVIFVTNNSGFSRILLSRQL
CCCCCCHHEEEECCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
SYLGLKVTPDMIITSGLAAAIYMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSVESERN
HHCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCEEEEECCCCCCC
LPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLWPAKDGLKLGAGALASSIIYA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHH
LRRDPDFIAGKPNTWIIEIAMRISSVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGI
HHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCCCCCCEEEEECC
STVDDVDRSSVKPKYVVNSLLDLL
CHHHCCHHHCCCHHHHHHHHHHHC
>Mature Secondary Structure 
STLNDYQLIISDVDGVIVREGEPIWENIQALRNIQNNGVKVIFVTNNSGFSRILLSRQL
CCCCCHHEEEECCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
SYLGLKVTPDMIITSGLAAAIYMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSVESERN
HHCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCEEEEECCCCCCC
LPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLWPAKDGLKLGAGALASSIIYA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHH
LRRDPDFIAGKPNTWIIEIAMRISSVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGI
HHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCHHHHHHHCCCCCCCCEEEEECC
STVDDVDRSSVKPKYVVNSLLDLL
CHHHCCHHHCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-nitrophenyl phosphate; H2O

Specific reaction: 4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA