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The map label for this gene is leuD [H]

Identifier: 227815749

GI number: 227815749

Start: 2890910

End: 2891491

Strand: Reverse

Name: leuD [H]

Synonym: BAMEG_3172

Alternate gene names: 227815749

Gene position: 2891491-2890910 (Counterclockwise)

Preceding gene: 227815750

Following gene: 227815748

Centisome position: 55.29

GC content: 34.02

Gene sequence:

>582_bases
ATGGAGCCATTTCGTATTCATAAAGGTACTGCCGCAGTACTAATGAATGATAACATTGATACGGATCAAATTATTCCGAA
GCAATATTTAAAGAGAATTGAAAGAACTGGGTTTGGAAAGTTTTTATTTGATGAGTGGCGATATGATAATGAGCGACACG
AAAACCCTAATTTCCCACTAAATGCACCAGATAGAAAAGGAGCAAGTATATTAATTACAGGTAATAATTTTGGATGTGGT
TCTTCAAGAGAACATGCACCTTGGGCACTTGCTGATTATGGTTTCCGCGTAATTATCGCCGGAGGATTTGCAGATATTTT
TTATATGAATTGTATGAAAAATGGTATGTTACCAATTGTAATGGATAAAGAAATGCGAGAGAAGCTTGTCAAAACGGATG
CGAGAGAACAAATAGAAGTAGATTTAGAGAATGAAGTCATTACAACGAGTACCCACAGATTCCATTTTACAATCGAGAAA
ATGTGGAAAGAGAAATTATTAAATGGTTTAGATGAAATTAGTATTACAATGCAATATGAACAAGAAATAAAAGAGTATGA
AAGAAGGATAGCTGCATATTGA

Upstream 100 bases:

>100_bases
AGACAAGGAAAAGGAGCACGAACGCATTTAGTTAGCCCAGCAATGGCGGCAGCAGCTGCGTTATATGGTCATTTTGTTGA
TATTAGAAAGGAGAGTTATG

Downstream 100 bases:

>100_bases
GATATAACGGAATGTTTAAAATGTATTTACATTCAGAATTATGTGTTATATACTATGTAAAAATTAGAGGAGAAAGGGAG
TAAGTATTATGAAAACAATG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNERHENPNFPLNAPDRKGASILITGNNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKEMREKLVKTDAREQIEVDLENEVITTSTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERRIAAY

Sequences:

>Translated_193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNERHENPNFPLNAPDRKGASILITGNNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKEMREKLVKTDAREQIEVDLENEVITTSTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERRIAAY
>Mature_193_residues
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNERHENPNFPLNAPDRKGASILITGNNFGCG
SSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKEMREKLVKTDAREQIEVDLENEVITTSTHRFHFTIEK
MWKEKLLNGLDEISITMQYEQEIKEYERRIAAY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=46.7005076142132, Blast_Score=186, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=47.4489795918367, Blast_Score=169, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22605; Mature: 22605

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNERHENPNFPL
CCCCEECCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC
NAPDRKGASILITGNNFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEEE
MDKEMREKLVKTDAREQIEVDLENEVITTSTHRFHFTIEKMWKEKLLNGLDEISITMQYE
ECHHHHHHHHHCCCCHHEEEECCCCEEEECCEEEEEHHHHHHHHHHHCCHHHEEEEEHHH
QEIKEYERRIAAY
HHHHHHHHHHCCC
>Mature Secondary Structure
MEPFRIHKGTAAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYDNERHENPNFPL
CCCCEECCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC
NAPDRKGASILITGNNFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEEE
MDKEMREKLVKTDAREQIEVDLENEVITTSTHRFHFTIEKMWKEKLLNGLDEISITMQYE
ECHHHHHHHHHCCCCHHEEEECCCCEEEECCEEEEEHHHHHHHHHHHCCHHHEEEEEHHH
QEIKEYERRIAAY
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]