| Definition | Vibrio cholerae M66-2 chromosome I, complete genome. |
|---|---|
| Accession | NC_012578 |
| Length | 2,892,523 |
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The map label for this gene is thrA [H]
Identifier: 227082488
GI number: 227082488
Start: 2456867
End: 2459344
Strand: Reverse
Name: thrA [H]
Synonym: VCM66_2287
Alternate gene names: 227082488
Gene position: 2459344-2456867 (Counterclockwise)
Preceding gene: 227082490
Following gene: 227082487
Centisome position: 85.02
GC content: 50.08
Gene sequence:
>2478_bases ATGCATTTGGAGGAAGGGATGCGAGTATTAAAGTTTGGAGGTTCATCGCTGGCGGATCCGGAGCGTTTTCTACGCGCTGC CGATATCATCGCGAATAACGCACAGCAGGAAGAGGTGGCCGTTGTCCTCTCTGCTCCGGGAAAAACCACCAATAAATTGG TGGCAGTGATTGAAAGTGCTTTACGTCAGGGAGAGGTGGAAGCGCAAATCGTAGAGCTAGAGAGCTCATTCTATGCCTTG CTTGATGGCATTAAAGCGCAGCTCCCGAATTTGGATGACAGTGCTTATCAACAGCAAGTGCACTCTTCCATGACTCAGTT ACGCCAGTTTGTGCATGGCATTACGTTGCTCGGTATGTGTCCAGACAATGTGAATGCACGCATTATCAGTAAAGGTGAAC GCGTTTCCATTCAATTGATGAAAGCGGTGATGGAAGCCAAAGGTCTGCCAGCGAATTTGGTTGACCCCGTTAAATACCTG CTTGCCAAAGGGGATCATCTTGAAGCTATGGTCGATGTGGAAATCTCGACTCAGCGTTTCAGACAAGCACCATTGCCACA ACAGCACGTCAATATCATGCCGGGCTTTACTGCGGGTAATGCACAGGGTGAGTTGGTCTGTTTAGGGCGTAACGGTTCGG ATTACTCAGCCGCAGTACTGGCTGCCTGTTTACGTGCGGATTGTTGTGAAATCTGGACGGATGTGGATGGAGTTTATAAC TGCGATCCGCGTTTGGTCGATGATGCGCGTCTTTTGAAATCCCTTAGCTATCAAGAGGCGATGGAGCTTTCTTACTTCGG AGCATCTGTGTTGCATCCGAAGACCATTGCACCGATTGCTCAATTTCAAATCCCTTGCCTGATCAAAAACAGCTTCAATC CACAAGGTGCGGGCACCTTGATTGGCCAAGACACTGGCGAAGATAAACTCGCGATCAAAGGCATTACCACTCTGAGCAAT CTGACCATGGTCAACGTTTCTGGTCCAGGAATGAAAGGCATGGTGGGGATGGCGAGCCGCGTGTTTGGCGCGATGTCAGC GGCAGATGTGTCGATCGTGCTCATTACTCAATCTTCTTCGGAATACAGCATCAGTTTCTGTATCGAAGCACAACATAAAG CTTTGGCACAGCAAGCGCTGGCGGATGCATTTGAACTGGAACTCAAAGATGGCCTGCTTGAGCCGGTTGAGTTTGTCGAT AACGTCGCCATCATCACGCTGGTTGGTGATGGTATGCGTACCTCTCGCGGTGTCGCGTCGCAATTTTTCTCATCTCTGGC CGAAGTACACGTCAACGTGATTGCGATTGCCCAAGGCTCTTCTGAGCGTGCGATTTCAGCGGTGATCCCCGATGATAAGA TTTCAGAAGCGATCAAGGCCTGTCACGAAAACCTCTTCAACTCTAAACACTTTTTAGATGTGTTTGTGGTCGGTGTTGGT GGCGTGGGCGGTGAGTTGGTGGATCAAATTCAGCGTCAACAAGCCAAGCTTGCGGAAAAAGGCATCATGATGCGCGTTTG TGGCCTCGCTAACAGTAAAGGCATGCTGCTTGATAGCCAAGGCTTGCCGTTAGAGCAGTGGCGCGATCGTATGATCAATG CGGATCAAGCGTTCAGTTTAGAGAATCTGGTGGCTTTGGTGCAGCGTAATCACATCATTAACCCAGTATTGGTGGATTGC ACGTCCAGTGATGAGATTGCTAACCAGTATGCGGATTTCCTCGCCGCGGGTTTCCATGTGGTGACCCCGAACAAGAAAGC CAATACCGCTAGCATGAGTTATTACCATCAGCTACGTAATGTGGCTCGTCATTCGCGTCGCAAACTGATGTATGAAACTA CAGTTGGCGCGGGTCTACCCGTTATCGAAAACTTGCAAAACCTAATTGCAGCAGGGGATGAGCTGGAAAAATTCAGCGGT ATTCTCTCAGGTTCTCTCTCCTTTATCTTCGGTAAATTGGATGAAGGCATGACCTTGAGCCAAGCAACGCAGCTTGCTAA AGAGAAAGGCTTTACAGAGCCAGATCCGCGCGATGATCTCTCTGGTATGGATGTGGCGCGCAAGTTACTGATTTTGGCGC GTGAAGCAGGGTACGAATTGGAGCTGAGCGATGTGGATGTTGAACAAGCACTGCCCGCTGGCTTTGATGCTTCAGGCAGT GTTGAGGAGTTCATGGCTCGTTTAGCGCAAGCCGATGCCGCGTTTGCCGAGCGAGTGGCGCAAGCCAAAGCCGAAGGCAA AGTGCTTCGCTATGTGGCGCAAATCGTCGATGGCCAGTGCCAAGTGCGGATTGTCGCGGTTGATGAAAACGATCCTATGT TCAAAGTCAAAGAAGGTGAAAACGCGCTGGCTTTCTACAGCCGTTACTATCAGCCAATCCCATTGGTATTACGTGGTTAC GGCGCGGGTTCTGAAGTGACTGCTGCAGGCGTGTTCTCAGATGTGATGCGTACACTCGGTTGGAAATTAGGGGTTTAA
Upstream 100 bases:
>100_bases TACCGACACGTCGCATGTTGGGGCAGGCTGCTGAGCGCAAAATTTCACAAAAAAGGCCTGTATCCCAACCGATACAGGCC TTTTTTTATACCCGCTTTTT
Downstream 100 bases:
>100_bases TAGAAATGAGTTCAGGTGACATGAGTGTTGTGGTGTACGCACCCGCTTCCATTGGTAATGTCAGTGTCGGGTTTGATGTG TTGGGGGCCGCAGTGTCCCC
Product: bifunctional aspartokinase I/homeserine dehydrogenase I
Products: NA
Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]
Number of amino acids: Translated: 825; Mature: 825
Protein sequence:
>825_residues MHLEEGMRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESALRQGEVEAQIVELESSFYAL LDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMCPDNVNARIISKGERVSIQLMKAVMEAKGLPANLVDPVKYL LAKGDHLEAMVDVEISTQRFRQAPLPQQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYN CDPRLVDDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTLIGQDTGEDKLAIKGITTLSN LTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSSEYSISFCIEAQHKALAQQALADAFELELKDGLLEPVEFVD NVAIITLVGDGMRTSRGVASQFFSSLAEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVG GVGGELVDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSLENLVALVQRNHIINPVLVDC TSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRNVARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEKFSG ILSGSLSFIFGKLDEGMTLSQATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGS VEEFMARLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGENALAFYSRYYQPIPLVLRGY GAGSEVTAAGVFSDVMRTLGWKLGV
Sequences:
>Translated_825_residues MHLEEGMRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESALRQGEVEAQIVELESSFYAL LDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMCPDNVNARIISKGERVSIQLMKAVMEAKGLPANLVDPVKYL LAKGDHLEAMVDVEISTQRFRQAPLPQQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYN CDPRLVDDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTLIGQDTGEDKLAIKGITTLSN LTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSSEYSISFCIEAQHKALAQQALADAFELELKDGLLEPVEFVD NVAIITLVGDGMRTSRGVASQFFSSLAEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVG GVGGELVDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSLENLVALVQRNHIINPVLVDC TSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRNVARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEKFSG ILSGSLSFIFGKLDEGMTLSQATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGS VEEFMARLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGENALAFYSRYYQPIPLVLRGY GAGSEVTAAGVFSDVMRTLGWKLGV >Mature_825_residues MHLEEGMRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESALRQGEVEAQIVELESSFYAL LDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMCPDNVNARIISKGERVSIQLMKAVMEAKGLPANLVDPVKYL LAKGDHLEAMVDVEISTQRFRQAPLPQQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYN CDPRLVDDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTLIGQDTGEDKLAIKGITTLSN LTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSSEYSISFCIEAQHKALAQQALADAFELELKDGLLEPVEFVD NVAIITLVGDGMRTSRGVASQFFSSLAEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVG GVGGELVDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSLENLVALVQRNHIINPVLVDC TSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRNVARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEKFSG ILSGSLSFIFGKLDEGMTLSQATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGS VEEFMARLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGENALAFYSRYYQPIPLVLRGY GAGSEVTAAGVFSDVMRTLGWKLGV
Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]
COG id: COG0527
COG function: function code E; Aspartokinases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 2 ACT domains [H]
Homologues:
Organism=Escherichia coli, GI1786183, Length=820, Percent_Identity=62.1951219512195, Blast_Score=1042, Evalue=0.0, Organism=Escherichia coli, GI1790376, Length=826, Percent_Identity=31.9612590799031, Blast_Score=363, Evalue=1e-101, Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=28.4164859002169, Blast_Score=160, Evalue=3e-40, Organism=Saccharomyces cerevisiae, GI6322599, Length=339, Percent_Identity=32.1533923303835, Blast_Score=169, Evalue=2e-42, Organism=Saccharomyces cerevisiae, GI6320893, Length=508, Percent_Identity=26.3779527559055, Blast_Score=149, Evalue=2e-36,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002912 - InterPro: IPR001048 - InterPro: IPR005106 - InterPro: IPR001341 - InterPro: IPR018042 - InterPro: IPR011147 - InterPro: IPR001342 - InterPro: IPR019811 - InterPro: IPR016040 [H]
Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]
EC number: =2.7.2.4; =1.1.1.3 [H]
Molecular weight: Translated: 89220; Mature: 89220
Theoretical pI: Translated: 4.79; Mature: 4.79
Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MHLEEGMRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESA CCHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH LRQGEVEAQIVELESSFYALLDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMC HHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEC PDNVNARIISKGERVSIQLMKAVMEAKGLPANLVDPVKYLLAKGDHLEAMVDVEISTQRF CCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECHHHH RQAPLPQQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYN HCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC CDPRLVDDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTL CCCCCCHHHHHHHHHCHHHHHHHHHCCCHHCCCCHHCCHHHHCCCEEEECCCCCCCCCEE IGQDTGEDKLAIKGITTLSNLTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSS EECCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC EYSISFCIEAQHKALAQQALADAFELELKDGLLEPVEFVDNVAIITLVGDGMRTSRGVAS CEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCCHHHHHH QFFSSLAEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVG HHHHHHHHHHEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC GVGGELVDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSL CCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCEEECCCCCHHHHHHHHCCCHHHHHH ENLVALVQRNHIINPVLVDCTSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRN HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH VARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEKFSGILSGSLSFIFGKLDEGMTLS HHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCCHH QATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGS HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCCCC VEEFMARLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGE HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCC NALAFYSRYYQPIPLVLRGYGAGSEVTAAGVFSDVMRTLGWKLGV CHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHCCC >Mature Secondary Structure MHLEEGMRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESA CCHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH LRQGEVEAQIVELESSFYALLDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMC HHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEC PDNVNARIISKGERVSIQLMKAVMEAKGLPANLVDPVKYLLAKGDHLEAMVDVEISTQRF CCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECHHHH RQAPLPQQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYN HCCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC CDPRLVDDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTL CCCCCCHHHHHHHHHCHHHHHHHHHCCCHHCCCCHHCCHHHHCCCEEEECCCCCCCCCEE IGQDTGEDKLAIKGITTLSNLTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSS EECCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC EYSISFCIEAQHKALAQQALADAFELELKDGLLEPVEFVDNVAIITLVGDGMRTSRGVAS CEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCCHHHHHH QFFSSLAEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVG HHHHHHHHHHEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC GVGGELVDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSL CCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCEEECCCCCHHHHHHHHCCCHHHHHH ENLVALVQRNHIINPVLVDCTSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRN HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH VARHSRRKLMYETTVGAGLPVIENLQNLIAAGDELEKFSGILSGSLSFIFGKLDEGMTLS HHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCCHH QATQLAKEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGS HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCCCC VEEFMARLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGE HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCC NALAFYSRYYQPIPLVLRGYGAGSEVTAAGVFSDVMRTLGWKLGV CHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8423151 [H]