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The map label for this gene is thrC [H]

Identifier: 227082486

GI number: 227082486

Start: 2454613

End: 2455893

Strand: Reverse

Name: thrC [H]

Synonym: VCM66_2285

Alternate gene names: 227082486

Gene position: 2455893-2454613 (Counterclockwise)

Preceding gene: 227082487

Following gene: 227082484

Centisome position: 84.9

GC content: 49.73

Gene sequence:

>1281_bases
ATGAAGCTGTACAACATAAAAGAAAATGATGAACAAGTCTCCTTTGGTCAAGCCGTTCGCCAAGGGTTAGGCCGCAATCA
AGGCCTGTTTTTTCCGGCACAATTGCCGAAGTTTGACGATATTGATGCTCTATTGGCCGAAGATTTCGTCTCTCGTAGCA
GCAAGATTTTATCGGCGTTGATTGGCGATGAACTGCCTGCGGATAAAATTCGCGCTATGGTGGATTCCGCCTTTCAATTC
GCCGCACCGATTGCCAAAGTGAAAGAGGGCGTTTACGCGCTCGAATTGTTCCACGGGCCAACGCTCGCGTTTAAAGATTT
TGGTGGTCGCTTTATGGCCCAGTCTCTGGCGGCGGTCTCGAACGGCGGAAAAATCACGATTTTGACGGCGACATCCGGTG
ATACCGGCGCTGCTGTTGCGCACGCATTTTATGGTATGCCAGACATTAATGTGGTGATCCTTTATCCGAAAGGAAAAATC
AGTCCGCTGCAAGAAAAGCTGTTCTGCACATTAGGTAAAAACATTCACACCGTGGCGATCAATGGCGATTTTGATGCTTG
CCAAGCACTAGTGAAACAAGCTTTTGATGACGCAGAGCTGCGCCAACAGATAGGTTTGAACTCTGCCAACTCAATTAACA
TCAGCCGTTTGATGGCACAAATCTGTTACTACTTTGAAGCCGCTGCGCAGATGAGCAAAGCTGAGCGTGAAAATCTGGTG
ATTTCAGTGCCTAGTGGCAACTTTGGTAACTTAACCGCAGGGCTGTTAGCCAAAGCACTGGGTTTACCGGTGAAGCGCTT
TATTGCGGCAACCAATGCCAACGATACAGTGCCACGTTACTTGGAAACGGGACGTTGGGAACCCAAACCCACGGTAGCAA
CAACGTCAAATGCGATGGATGTCAGCCAACCGAACAACTGGCCGCGTATCGAAGAGCTCTGCCAACGTATGGGATGGGGC
TTAGAAACGCTGGGCAAAGGCGCGGTTTCTGACGAGGAAAGTGCGCAAGCCGTACAAGAACTTTACGATCTAGGCTACTT
GTGTGAACCGCATGGTTCGATTGCTTACCGTGTATTGGAAGAGCAGCGCCAGCCGCAAGAAACGGGGCTGTTCTTATGTA
CCGCGCATCCCGCCAAATTCAAAGAAGTGGTGGACGATATTTTAGGTACCGACATTGCTCTACCAGCGCCATTGGCAAAA
CATGCGGCGATGGAATTGCTCTCACAAGAGCTGAATGCCGATTTCGCAGCGCTGCGTGCGTTACTGGTGAATGTGAAATA
A

Upstream 100 bases:

>100_bases
TGGCACGTTGGCTTGAGCAAAACTATGTGCAAAATGAAGAAGGATTCGTCCACATTTGTCGTCTAGACAAGCAAGGTTCG
AAAGTAACAGGAAGTGAGCT

Downstream 100 bases:

>100_bases
TTCGCGGATTTGAGAAAGCGAAGATAATAAAAAACGGTGCTCATTGAGCACCGTTTTAGTATGGACTCTTTACTGACGAT
TACAGAGATTCAGTGAAAGT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPAQLPKFDDIDALLAEDFVSRSSKILSALIGDELPADKIRAMVDSAFQF
AAPIAKVKEGVYALELFHGPTLAFKDFGGRFMAQSLAAVSNGGKITILTATSGDTGAAVAHAFYGMPDINVVILYPKGKI
SPLQEKLFCTLGKNIHTVAINGDFDACQALVKQAFDDAELRQQIGLNSANSINISRLMAQICYYFEAAAQMSKAERENLV
ISVPSGNFGNLTAGLLAKALGLPVKRFIAATNANDTVPRYLETGRWEPKPTVATTSNAMDVSQPNNWPRIEELCQRMGWG
LETLGKGAVSDEESAQAVQELYDLGYLCEPHGSIAYRVLEEQRQPQETGLFLCTAHPAKFKEVVDDILGTDIALPAPLAK
HAAMELLSQELNADFAALRALLVNVK

Sequences:

>Translated_426_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPAQLPKFDDIDALLAEDFVSRSSKILSALIGDELPADKIRAMVDSAFQF
AAPIAKVKEGVYALELFHGPTLAFKDFGGRFMAQSLAAVSNGGKITILTATSGDTGAAVAHAFYGMPDINVVILYPKGKI
SPLQEKLFCTLGKNIHTVAINGDFDACQALVKQAFDDAELRQQIGLNSANSINISRLMAQICYYFEAAAQMSKAERENLV
ISVPSGNFGNLTAGLLAKALGLPVKRFIAATNANDTVPRYLETGRWEPKPTVATTSNAMDVSQPNNWPRIEELCQRMGWG
LETLGKGAVSDEESAQAVQELYDLGYLCEPHGSIAYRVLEEQRQPQETGLFLCTAHPAKFKEVVDDILGTDIALPAPLAK
HAAMELLSQELNADFAALRALLVNVK
>Mature_426_residues
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPAQLPKFDDIDALLAEDFVSRSSKILSALIGDELPADKIRAMVDSAFQF
AAPIAKVKEGVYALELFHGPTLAFKDFGGRFMAQSLAAVSNGGKITILTATSGDTGAAVAHAFYGMPDINVVILYPKGKI
SPLQEKLFCTLGKNIHTVAINGDFDACQALVKQAFDDAELRQQIGLNSANSINISRLMAQICYYFEAAAQMSKAERENLV
ISVPSGNFGNLTAGLLAKALGLPVKRFIAATNANDTVPRYLETGRWEPKPTVATTSNAMDVSQPNNWPRIEELCQRMGWG
LETLGKGAVSDEESAQAVQELYDLGYLCEPHGSIAYRVLEEQRQPQETGLFLCTAHPAKFKEVVDDILGTDIALPAPLAK
HAAMELLSQELNADFAALRALLVNVK

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI153792148, Length=421, Percent_Identity=29.6912114014252, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI149193323, Length=422, Percent_Identity=28.436018957346, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1786185, Length=426, Percent_Identity=68.075117370892, Blast_Score=579, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6319901, Length=448, Percent_Identity=32.5892857142857, Blast_Score=216, Evalue=4e-57,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 46229; Mature: 46229

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPAQLPKFDDIDALLAEDFVSRSSKILSAL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGDELPADKIRAMVDSAFQFAAPIAKVKEGVYALELFHGPTLAFKDFGGRFMAQSLAAVS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHCCCHHHHHHHHHHC
NGGKITILTATSGDTGAAVAHAFYGMPDINVVILYPKGKISPLQEKLFCTLGKNIHTVAI
CCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
NGDFDACQALVKQAFDDAELRQQIGLNSANSINISRLMAQICYYFEAAAQMSKAERENLV
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
ISVPSGNFGNLTAGLLAKALGLPVKRFIAATNANDTVPRYLETGRWEPKPTVATTSNAMD
EECCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEECCCCCC
VSQPNNWPRIEELCQRMGWGLETLGKGAVSDEESAQAVQELYDLGYLCEPHGSIAYRVLE
CCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHH
EQRQPQETGLFLCTAHPAKFKEVVDDILGTDIALPAPLAKHAAMELLSQELNADFAALRA
HHHCCHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LLVNVK
HHHCCC
>Mature Secondary Structure
MKLYNIKENDEQVSFGQAVRQGLGRNQGLFFPAQLPKFDDIDALLAEDFVSRSSKILSAL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGDELPADKIRAMVDSAFQFAAPIAKVKEGVYALELFHGPTLAFKDFGGRFMAQSLAAVS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHCCCHHHHHHHHHHC
NGGKITILTATSGDTGAAVAHAFYGMPDINVVILYPKGKISPLQEKLFCTLGKNIHTVAI
CCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEE
NGDFDACQALVKQAFDDAELRQQIGLNSANSINISRLMAQICYYFEAAAQMSKAERENLV
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
ISVPSGNFGNLTAGLLAKALGLPVKRFIAATNANDTVPRYLETGRWEPKPTVATTSNAMD
EECCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEECCCCCC
VSQPNNWPRIEELCQRMGWGLETLGKGAVSDEESAQAVQELYDLGYLCEPHGSIAYRVLE
CCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHH
EQRQPQETGLFLCTAHPAKFKEVVDDILGTDIALPAPLAKHAAMELLSQELNADFAALRA
HHHCCHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LLVNVK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]