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The map label for this gene is pdxB [H]

Identifier: 227082233

GI number: 227082233

Start: 2201826

End: 2202989

Strand: Reverse

Name: pdxB [H]

Synonym: VCM66_2031

Alternate gene names: 227082233

Gene position: 2202989-2201826 (Counterclockwise)

Preceding gene: 227082234

Following gene: 227082232

Centisome position: 76.16

GC content: 49.91

Gene sequence:

>1164_bases
TTGCCGCAAATTCCCACTATGAAAATTCTGATTGATGAGAACATGCCTTACGCTCAAGCGCTGTTTAGTCAGCTTGGCGA
AGTGATCTTAAAACCCGGCCGTACCTTAACCGCCGATGATCTGATTGATGTCGATGCCTTAATGATTCGCTCAGTCACCA
AAGTCAATGACGCTCTCCTTGCTAAAGCGAATCGCTTGAAGTTCGTGGGTACCGCGACCGCGGGTATGGACCATGTTGAC
CAAGCTCTGTTAAGAGAGCGTGGCATCTTCTTCACGGCGGCTCCTGGCTGTAACAAAGTCGGTGTTGCGGAATACGTGTT
TAGCGTGCTCATGGTGCTTGCGCAGCAGCAAGGGTTCTCGGTTTTCGATAAAACGGTAGGCATCATCGGTGCTGGCCAAG
TGGGTAGCTATCTTGCGAAGTGTCTTTCTGGCATTGGCATGAAAGTGCTGCTCAATGATCCGCCTAAGCAAGCGCAAGGT
GATGAGCGAGAATTTACCGAGCTGGAAACCTTACTTAAACAAGCGGATGTGATCACTCTGCATACGCCGATTACCCGCGG
CGGTGAGTGGCCAACCCATCATCTGATTGATGCGGCAATTCTTGAGCAACTGCGCAGTGACCAGATTTTAATTAATGCGG
CGCGTGGCCCTGTAGTGGATAACGCGGCGCTGAAAGCGCGTTTACAACAAGGCGATGGTTTTACCGCTGTATTAGACGTG
TTTGAGTTTGAACCGCAAGTGGATATGGAATTGCTACCGCTGCTCGCATTTGCCACTCCGCATATTGCAGGTTATGGCCT
TGAAGGTAAGGCGCGTGGTACCACCATGATTTTCAATAGCTACTGCGAGTTTCTTGGCAGTGCACACTGTGCAAACCCAG
CAAGCTTGTTACCCAAAGCGCCTGTGCCCAAAGTTTATCTAGAGCGAGCATGGGACGAGGAAACACTGCGTACGCTAACT
CAAATTATTTATGATGTGCGTAAAGATGACGCACAGTTTCGCCGCGAGATTCACCAACCCGGAGCGTTTGACTTGATGCG
CAAACACTATTGGGATAGACGTGAATACAGCGCGGTGACTCTAGCTGGAGGCGCTGATTGCCATTTGGCTCCTTTGGCTA
AACTCGGTTTTCAAGTAGAGGTATGTGATGAGCCAACAATTTAA

Upstream 100 bases:

>100_bases
GGATCCTTTTGTTCTGGATGTTTGCCCGGCGGCCTAGGTCGTCGGGTATTTTTATTCTCGACAGCCTCCGTGCTTTTCTG
CACAATGCCTGCAACTTTGA

Downstream 100 bases:

>100_bases
TGTTGCCATTTTTGGCGCAACCGGCGCTGTAGGCGAAACCATGTTGGAAGTACTGCAGGAGCGTGAGTTTCCTGTGGATG
AACTTTTCCTGCTGGCCAGT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALLAKANRLKFVGTATAGMDHVD
QALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQG
DEREFTELETLLKQADVITLHTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDV
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLERAWDEETLRTLT
QIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI

Sequences:

>Translated_387_residues
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALLAKANRLKFVGTATAGMDHVD
QALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQG
DEREFTELETLLKQADVITLHTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDV
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLERAWDEETLRTLT
QIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI
>Mature_386_residues
PQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALLAKANRLKFVGTATAGMDHVDQ
ALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFSVFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGD
EREFTELETLLKQADVITLHTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDVF
EFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKAPVPKVYLERAWDEETLRTLTQ
IIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVTLAGGADCHLAPLAKLGFQVEVCDEPTI

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=292, Percent_Identity=28.7671232876712, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI145580575, Length=154, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI61743967, Length=151, Percent_Identity=38.4105960264901, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557497, Length=151, Percent_Identity=37.7483443708609, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788660, Length=372, Percent_Identity=49.7311827956989, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=33.2, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787645, Length=210, Percent_Identity=29.5238095238095, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=29.4573643410853, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=310, Percent_Identity=30, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=354, Percent_Identity=28.2485875706215, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=354, Percent_Identity=27.9661016949153, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=167, Percent_Identity=29.3413173652695, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=270, Percent_Identity=26.2962962962963, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=275, Percent_Identity=25.8181818181818, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574286, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=25.9398496240602, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585514, Length=266, Percent_Identity=25.9398496240602, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574282, Length=266, Percent_Identity=25.9398496240602, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45551003, Length=266, Percent_Identity=25.9398496240602, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574284, Length=266, Percent_Identity=25.9398496240602, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646446, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646448, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646452, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646450, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472511, Length=254, Percent_Identity=27.9527559055118, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 42534; Mature: 42402

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALL
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AKANRLKFVGTATAGMDHVDQALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFS
HHHCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
VFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGDEREFTELETLLKQADVITL
HHHHHHCEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEE
HTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDV
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKA
HHCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCC
PVPKVYLERAWDEETLRTLTQIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVT
CCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCEEE
LAGGADCHLAPLAKLGFQVEVCDEPTI
EECCCCCCHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure 
PQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALL
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
AKANRLKFVGTATAGMDHVDQALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFS
HHHCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
VFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGDEREFTELETLLKQADVITL
HHHHHHCEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEE
HTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDV
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKA
HHCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCC
PVPKVYLERAWDEETLRTLTQIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVT
CCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHCCEEE
LAGGADCHLAPLAKLGFQVEVCDEPTI
EECCCCCCHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]