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The map label for this gene is hlyB [H]

Identifier: 227082094

GI number: 227082094

Start: 2054942

End: 2056996

Strand: Direct

Name: hlyB [H]

Synonym: VCM66_1891

Alternate gene names: 227082094

Gene position: 2054942-2056996 (Clockwise)

Preceding gene: 227082093

Following gene: 227082097

Centisome position: 71.04

GC content: 43.89

Gene sequence:

>2055_bases
GTGTCATCCGGTAAATATGATGGGTTAGAGGACTCTTTCATGAGAGTAAAAACAAAAATATTAGTCACATTCAGCGTCGT
AGGTATTTTAGTTACTGCTTTAGGCATTTGGACAATAAAAACCATGTACTCAAGCTCCTCGGGGTTGAGCTACATTGTTG
GCCCCGCTTGGGATACGGCAGATGGGGCGATGGAAACCACCATACAGATTGAAGCTCAGATGCTTGCCGTCAATAGGCTC
ATCCTAGGCGAAGATAGCCAACGGGTTGAACAAATACTCAATACTGCCATTACAGATGTCGATACGTCCTCTTCCCGCAT
GATCGAAGCGGGTCTATTAAGTGCGGCTCAGACACAACAATTTTCTCAATTCAACTCACAGTATCAACAATCAAGAAACG
CACTGATTACTAGCTACAAGAACTATATCGAGACCAAAAAATCCTACGATAAAGCAACGCAAGTCCTTGTCGATTTTGGT
GAAAAACTAGAGACATTAGGTGATAGTGCCGTTGAGGAATTAGAACGAGAACCTAATCGGAACATCACTTGGCAAAGTGA
TGTTATGGAACGCTGGCAAGCCGCAGATGGCGGTATGGAATCCAATATAGGCTTGCTATGGAAGCTCTACTACACACAAC
GTTTGCTGGATGGTCAAGATGATGCCACTCAAATTAAAGCCATTGAACAAGCGATTGGATTTCAAAAACAAGCCAACAGC
GAAATGTTCTCGACTGGCCGATTTACTATATCGGCAGGAGAGGAATGGAAAAATGCAAGTTATGAAGAAGTATTCTCCCA
ATTAAACTCGCAGCATGAACAGGCGATGATGGCGGTCATTGAAAGCTATCGGAACTATCGTCAAATCTACCAAGAGTACA
CAGTAACATCACTGGCATTACTGGATTTCATTGCCGAATTAGAAGAACTCGCAGACAGTAAAGTAGAGCAGCAAGCCGTG
CTTATTTTAGATGGACAAGCATGGGCGATATCTTCATTTAAGGTATTGATCATTATCGCTTTGCTTGTATTACTGTTGTT
AGGTTGGATCCTTGTGAACCAAATTCTATCGCCTATACAACGTTTGCAGGAGAGAGTAACCGATATCTCTGAAGGTAATG
GTGACTTAACCCTGCGAGTGAACATCACCACCCAAGATGAGTTTGGAGAATTGGGCAAATCATTCGATAAATTTATCGAA
AAAATACAGAATCTTATTGCTGACATCACTCAATCTACCAATCTCGCTAAGACCGCGGCGGTAGACCTGTCTGCCACGTT
TAAAGTGACCGCAGAAGCCGTCAATAAACAGACATTCGAAGTGAATACTATTTCGAACGCGACCACGGCGATGACCGCCA
TTTCTTCGCAAGTGATCAGTGGCGCGCGAGAGATAAGCCAATCGGTACTTAACATAGATAAAAACGCGCAAAGTACACTC
AGCAACGTTCGACAAGCCGCTCAATCGGTCAATGAACTGGTTGCAGAGGTGACACAAGGAACTGAAACCATCAACTCCTT
AAAAAATCATGTCACAAGTATCGAGCCAGTTTTAGCCGACATTAATGGCATTGCAGAACAAACTAATTTGCTCGCACTTA
ATGCAGCGATTGAAGCCGCTCGTGCGGGAGAACAAGGGCGAGGCTTTGCGGTGGTTGCCGATGAAGTGCGTTCACTTGCT
ACACGAACACAAGGCAGTACCAACACCATCCAGCAGAGCATTACTCAATTGCGTAGCAGCGCCGATGAATCGGTAAGAGT
TATTAACAACAGCATGTTAAAAGGTACTCAAACCACAGAGATCACATCACAGGCTGAAGAGTCCTTACATCAAGTCGCGA
TTGAAATCTCTCGACTAACGCAGATGAACCAGCAGACCTCTGAGGCGATTACTCATCAAGAGCAATCGGTGACCAGTATT
GCTTCTAGCCTTTCTCACTTACAAGCACTTTGCCAAAGTGCCCAAGAAAAAATCCAACAGAGTGAGCACACCATTTCAGC
ACTCAAGCTAAAACAAGAAGATTTGGCGCTAAAGATGAGTAAATTCAAAATTTAG

Upstream 100 bases:

>100_bases
CCCATTATTTATCCTCCCAATTAACCAATAACATCACCAAAACTGTTCTAGACTCAAAGTCACATTACCGACTTTTTCAG
CCTTTCGTTTAGCACCAACA

Downstream 100 bases:

>100_bases
TATCCTCTGGCAGTGGGTGACTTACCTATGCCAAAACCGCCCACTGCACCTGTTAGCCGCGCCTTGGGTACGCAACAATA
TTTTGAAACACTGCTTTTTC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MSSGKYDGLEDSFMRVKTKILVTFSVVGILVTALGIWTIKTMYSSSSGLSYIVGPAWDTADGAMETTIQIEAQMLAVNRL
ILGEDSQRVEQILNTAITDVDTSSSRMIEAGLLSAAQTQQFSQFNSQYQQSRNALITSYKNYIETKKSYDKATQVLVDFG
EKLETLGDSAVEELEREPNRNITWQSDVMERWQAADGGMESNIGLLWKLYYTQRLLDGQDDATQIKAIEQAIGFQKQANS
EMFSTGRFTISAGEEWKNASYEEVFSQLNSQHEQAMMAVIESYRNYRQIYQEYTVTSLALLDFIAELEELADSKVEQQAV
LILDGQAWAISSFKVLIIIALLVLLLLGWILVNQILSPIQRLQERVTDISEGNGDLTLRVNITTQDEFGELGKSFDKFIE
KIQNLIADITQSTNLAKTAAVDLSATFKVTAEAVNKQTFEVNTISNATTAMTAISSQVISGAREISQSVLNIDKNAQSTL
SNVRQAAQSVNELVAEVTQGTETINSLKNHVTSIEPVLADINGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLA
TRTQGSTNTIQQSITQLRSSADESVRVINNSMLKGTQTTEITSQAEESLHQVAIEISRLTQMNQQTSEAITHQEQSVTSI
ASSLSHLQALCQSAQEKIQQSEHTISALKLKQEDLALKMSKFKI

Sequences:

>Translated_684_residues
MSSGKYDGLEDSFMRVKTKILVTFSVVGILVTALGIWTIKTMYSSSSGLSYIVGPAWDTADGAMETTIQIEAQMLAVNRL
ILGEDSQRVEQILNTAITDVDTSSSRMIEAGLLSAAQTQQFSQFNSQYQQSRNALITSYKNYIETKKSYDKATQVLVDFG
EKLETLGDSAVEELEREPNRNITWQSDVMERWQAADGGMESNIGLLWKLYYTQRLLDGQDDATQIKAIEQAIGFQKQANS
EMFSTGRFTISAGEEWKNASYEEVFSQLNSQHEQAMMAVIESYRNYRQIYQEYTVTSLALLDFIAELEELADSKVEQQAV
LILDGQAWAISSFKVLIIIALLVLLLLGWILVNQILSPIQRLQERVTDISEGNGDLTLRVNITTQDEFGELGKSFDKFIE
KIQNLIADITQSTNLAKTAAVDLSATFKVTAEAVNKQTFEVNTISNATTAMTAISSQVISGAREISQSVLNIDKNAQSTL
SNVRQAAQSVNELVAEVTQGTETINSLKNHVTSIEPVLADINGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLA
TRTQGSTNTIQQSITQLRSSADESVRVINNSMLKGTQTTEITSQAEESLHQVAIEISRLTQMNQQTSEAITHQEQSVTSI
ASSLSHLQALCQSAQEKIQQSEHTISALKLKQEDLALKMSKFKI
>Mature_683_residues
SSGKYDGLEDSFMRVKTKILVTFSVVGILVTALGIWTIKTMYSSSSGLSYIVGPAWDTADGAMETTIQIEAQMLAVNRLI
LGEDSQRVEQILNTAITDVDTSSSRMIEAGLLSAAQTQQFSQFNSQYQQSRNALITSYKNYIETKKSYDKATQVLVDFGE
KLETLGDSAVEELEREPNRNITWQSDVMERWQAADGGMESNIGLLWKLYYTQRLLDGQDDATQIKAIEQAIGFQKQANSE
MFSTGRFTISAGEEWKNASYEEVFSQLNSQHEQAMMAVIESYRNYRQIYQEYTVTSLALLDFIAELEELADSKVEQQAVL
ILDGQAWAISSFKVLIIIALLVLLLLGWILVNQILSPIQRLQERVTDISEGNGDLTLRVNITTQDEFGELGKSFDKFIEK
IQNLIADITQSTNLAKTAAVDLSATFKVTAEAVNKQTFEVNTISNATTAMTAISSQVISGAREISQSVLNIDKNAQSTLS
NVRQAAQSVNELVAEVTQGTETINSLKNHVTSIEPVLADINGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAT
RTQGSTNTIQQSITQLRSSADESVRVINNSMLKGTQTTEITSQAEESLHQVAIEISRLTQMNQQTSEAITHQEQSVTSIA
SSLSHLQALCQSAQEKIQQSEHTISALKLKQEDLALKMSKFKI

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=319, Percent_Identity=24.7648902821317, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787690, Length=328, Percent_Identity=26.219512195122, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788195, Length=287, Percent_Identity=28.2229965156794, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789453, Length=355, Percent_Identity=25.0704225352113, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788194, Length=329, Percent_Identity=26.4437689969605, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 75396; Mature: 75264

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGKYDGLEDSFMRVKTKILVTFSVVGILVTALGIWTIKTMYSSSSGLSYIVGPAWDTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
DGAMETTIQIEAQMLAVNRLILGEDSQRVEQILNTAITDVDTSSSRMIEAGLLSAAQTQQ
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FSQFNSQYQQSRNALITSYKNYIETKKSYDKATQVLVDFGEKLETLGDSAVEELEREPNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NITWQSDVMERWQAADGGMESNIGLLWKLYYTQRLLDGQDDATQIKAIEQAIGFQKQANS
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCC
EMFSTGRFTISAGEEWKNASYEEVFSQLNSQHEQAMMAVIESYRNYRQIYQEYTVTSLAL
HHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDFIAELEELADSKVEQQAVLILDGQAWAISSFKVLIIIALLVLLLLGWILVNQILSPIQ
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQERVTDISEGNGDLTLRVNITTQDEFGELGKSFDKFIEKIQNLIADITQSTNLAKTAA
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VDLSATFKVTAEAVNKQTFEVNTISNATTAMTAISSQVISGAREISQSVLNIDKNAQSTL
CCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SNVRQAAQSVNELVAEVTQGTETINSLKNHVTSIEPVLADINGIAEQTNLLALNAAIEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGEQGRGFAVVADEVRSLATRTQGSTNTIQQSITQLRSSADESVRVINNSMLKGTQTTE
HCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHH
ITSQAEESLHQVAIEISRLTQMNQQTSEAITHQEQSVTSIASSLSHLQALCQSAQEKIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEHTISALKLKQEDLALKMSKFKI
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSGKYDGLEDSFMRVKTKILVTFSVVGILVTALGIWTIKTMYSSSSGLSYIVGPAWDTA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
DGAMETTIQIEAQMLAVNRLILGEDSQRVEQILNTAITDVDTSSSRMIEAGLLSAAQTQQ
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
FSQFNSQYQQSRNALITSYKNYIETKKSYDKATQVLVDFGEKLETLGDSAVEELEREPNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NITWQSDVMERWQAADGGMESNIGLLWKLYYTQRLLDGQDDATQIKAIEQAIGFQKQANS
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCC
EMFSTGRFTISAGEEWKNASYEEVFSQLNSQHEQAMMAVIESYRNYRQIYQEYTVTSLAL
HHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDFIAELEELADSKVEQQAVLILDGQAWAISSFKVLIIIALLVLLLLGWILVNQILSPIQ
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQERVTDISEGNGDLTLRVNITTQDEFGELGKSFDKFIEKIQNLIADITQSTNLAKTAA
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VDLSATFKVTAEAVNKQTFEVNTISNATTAMTAISSQVISGAREISQSVLNIDKNAQSTL
CCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SNVRQAAQSVNELVAEVTQGTETINSLKNHVTSIEPVLADINGIAEQTNLLALNAAIEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGEQGRGFAVVADEVRSLATRTQGSTNTIQQSITQLRSSADESVRVINNSMLKGTQTTE
HCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHH
ITSQAEESLHQVAIEISRLTQMNQQTSEAITHQEQSVTSIASSLSHLQALCQSAQEKIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEHTISALKLKQEDLALKMSKFKI
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]