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The map label for this gene is minD [H]

Identifier: 227082089

GI number: 227082089

Start: 2048800

End: 2049630

Strand: Direct

Name: minD [H]

Synonym: VCM66_1884

Alternate gene names: 227082089

Gene position: 2048800-2049630 (Clockwise)

Preceding gene: 227082088

Following gene: 227082090

Centisome position: 70.83

GC content: 48.98

Gene sequence:

>831_bases
TTGAAAGGAAAAAATAACATGTCGCGCATTATTGTCGTCACGTCAGGAAAAGGCGGGGTTGGCAAAACCACCTCCAGTGC
TGCAATTGCTTCAGGTCTCGCGCTGCGAGGCAAAAAAACCGCTGTGATCGATTTTGATATCGGTCTGCGTAATCTCGATT
TAATCATGGGCTGTGAGCGCCGCGTGGTGTACGATTTTGTCAATGTCATCAACGGTGAAGCAACGCTCAACCAAGCCTTG
ATTAAAGACAAACGCAACGAAAACCTGTTCATCCTGCCTGCCTCTCAAACTCGTGATAAAGATGCGCTAACCAAAGATGG
TGTACAGCGCGTGCTCAATGATCTCAAAGAGATGGGTTTTGACTTCATCATTTGTGACTCACCAGCTGGGATTGAGCAAG
GCGCGCTGATGGCGTTGTACTACGCCGACGAAGCGATTGTCACCACCAACCCTGAAGTATCGTCAGTGCGCGACTCTGAC
CGTATTCTAGGCATTCTCGATTCTAAATCGATGCGGGCGGAACAAGGCCAAGCGCCGATCAAACAGCATCTGCTGCTCAC
TCGCTACAACCCTGCACGCGTCACTCAAGGTGAAATGCTCAGTGTGCAAGACGTCGAAGAAATCCTACATGTGCCGCTGC
TCGGTGTGATTCCAGAAAGCCAAGCCGTATTGAACGCCTCGAACAAAGGGGTTCCAGTAATTTTTGACGATCAATCCGAT
GCAGGCCAAGCTTATCAAGATACGGTGGCACGTCTACTTGGTGAACAAGTTGAGTTTCGTTTCCTGACTGAAGCGAAGAA
AGGTATTTTCAAACGCTTATTCGGAGGTTAA

Upstream 100 bases:

>100_bases
ATTGGTTAAGTGATCAAATTGAAAGTCAGTTCTGGCAACAGAGGGTGATGCTCAGTATGACTGACGAATCGCTCTATCTC
GAAACGCTCACGATTTAAGA

Downstream 100 bases:

>100_bases
CGCTATGTCATTGCTGGAATTTTTCCGCCCACAGAAGAAGACTTCCGCGAGCGTTGCCAAAGAGCGTCTGCAGATCATTG
TGGCTGAGCGTCGTAGCCAA

Product: septum site-determining protein MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKGKNNMSRIIVVTSGKGGVGKTTSSAAIASGLALRGKKTAVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEATLNQAL
IKDKRNENLFILPASQTRDKDALTKDGVQRVLNDLKEMGFDFIICDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSD
RILGILDSKSMRAEQGQAPIKQHLLLTRYNPARVTQGEMLSVQDVEEILHVPLLGVIPESQAVLNASNKGVPVIFDDQSD
AGQAYQDTVARLLGEQVEFRFLTEAKKGIFKRLFGG

Sequences:

>Translated_276_residues
MKGKNNMSRIIVVTSGKGGVGKTTSSAAIASGLALRGKKTAVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEATLNQAL
IKDKRNENLFILPASQTRDKDALTKDGVQRVLNDLKEMGFDFIICDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSD
RILGILDSKSMRAEQGQAPIKQHLLLTRYNPARVTQGEMLSVQDVEEILHVPLLGVIPESQAVLNASNKGVPVIFDDQSD
AGQAYQDTVARLLGEQVEFRFLTEAKKGIFKRLFGG
>Mature_276_residues
MKGKNNMSRIIVVTSGKGGVGKTTSSAAIASGLALRGKKTAVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEATLNQAL
IKDKRNENLFILPASQTRDKDALTKDGVQRVLNDLKEMGFDFIICDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSD
RILGILDSKSMRAEQGQAPIKQHLLLTRYNPARVTQGEMLSVQDVEEILHVPLLGVIPESQAVLNASNKGVPVIFDDQSD
AGQAYQDTVARLLGEQVEFRFLTEAKKGIFKRLFGG

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=270, Percent_Identity=78.5185185185185, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 30099; Mature: 30099

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKNNMSRIIVVTSGKGGVGKTTSSAAIASGLALRGKKTAVIDFDIGLRNLDLIMGCER
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCEECCCCEEEEEECCCCCCCHHHHCCCH
RVVYDFVNVINGEATLNQALIKDKRNENLFILPASQTRDKDALTKDGVQRVLNDLKEMGF
HHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
DFIICDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSDRILGILDSKSMRAEQGQAPI
EEEEECCCCCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCEEEEECCCCHHHHCCCCCH
KQHLLLTRYNPARVTQGEMLSVQDVEEILHVPLLGVIPESQAVLNASNKGVPVIFDDQSD
HHCEEEEECCCCCCCCCCEECHHHHHHHHHCCCEEECCCCHHEEECCCCCCEEEECCCCC
AGQAYQDTVARLLGEQVEFRFLTEAKKGIFKRLFGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGKNNMSRIIVVTSGKGGVGKTTSSAAIASGLALRGKKTAVIDFDIGLRNLDLIMGCER
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCEECCCCEEEEEECCCCCCCHHHHCCCH
RVVYDFVNVINGEATLNQALIKDKRNENLFILPASQTRDKDALTKDGVQRVLNDLKEMGF
HHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
DFIICDSPAGIEQGALMALYYADEAIVTTNPEVSSVRDSDRILGILDSKSMRAEQGQAPI
EEEEECCCCCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCEEEEECCCCHHHHCCCCCH
KQHLLLTRYNPARVTQGEMLSVQDVEEILHVPLLGVIPESQAVLNASNKGVPVIFDDQSD
HHCEEEEECCCCCCCCCCEECHHHHHHHHHCCCEEECCCCHHEEECCCCCCEEEECCCCC
AGQAYQDTVARLLGEQVEFRFLTEAKKGIFKRLFGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]