Click here to switch to the map view.

The map label for this gene is ald [H]

Identifier: 227082035

GI number: 227082035

Start: 1992549

End: 1993673

Strand: Direct

Name: ald [H]

Synonym: VCM66_1829

Alternate gene names: 227082035

Gene position: 1992549-1993673 (Clockwise)

Preceding gene: 227082032

Following gene: 227082039

Centisome position: 68.89

GC content: 48.27

Gene sequence:

>1125_bases
ATGATCATTGGCGTTCCTAAAGAAATCAAGAACCACGAATATCGTGTGGGCATGATTCCAGCTAGCGTGCGGGAGCTTGT
CTCACACGGACACCAAGTTTACGTAGAAACAAATGCCGGTTCCGGCATCGGCTTTTCAGACGATGATTACATCGCTGTAG
GCGCATCCATTCTTCCTACCGCTGCAGACGTCTTTGCGAAAGCAGAGATGATTGTAAAGGTTAAAGAACCTCAAGCTATC
GAGCGAGCCATGCTCAAAGAGGGGCAAATTTTATTTACTTATTTGCACCTAGCTCCAGATTTTCCACAAACTGAAGAGCT
AATCAAGAGCAAAGCTGTCTGCATAGCCTATGAGACTGTAACAGATAATATGGGTCGCTTACCACTACTGGCACCGATGT
CTGAAGTCGCAGGCCGTATGTCCATTCAGGCTGGCGCTCAAACCTTAGAAAAATCACGTGGCGGCAGTGGCTTATTACTC
GGTGGCGTTCCCGGTGTTGCTCCTGCAAAAGTCGTAGTACTCGGCGGCGGTGTAGTCGGCGCTAACGCAGCCCGTATGGC
TATTGGCCTACGTGCCGATGTCACTATTCTAGATCGCAACATCGATACTCTACGTAAACTCGATGAAGAGTTTCAAGGCC
GAGCTAACGTCGTATATTCCACCGCAGATGCCATCGAAAAATACGTATTAGAAGCAGACTTAGTGATCGGTGCCGTTCTT
ATCCCTGGTGCTGCTGCGCCTAAACTGGTCACTCAGCAACACATCAAACGCATGAAACCCGGTTCAGCCGTAGTTGATGT
CGCGATCGACCAAGGGGGTTGCTTCGAAACCTCTCACCCTACCACTCACGCAGAGCCCACTTATATTGTTGATGATGTGG
TGCACTACTGTGTAGCCAATATGCCCGGCGCTGTGGCGCGCACTTCTACCTTTGCTCTTAATAACGCCACGCTACCATAT
ATCGTTAAACTCGCCAATAAAGGCTACCAAAAAGCCCTACTAGAAGATGCAGGATTTTTAAAAGGCCTCAACGTAATTCA
CGGAAAAGTGACTTACAAAGAGGTTGCAGAAAACTTTGGCCTCGATTATGTCGACCCCGCTAAAGCGATTGCGATGTTTA
ATTAA

Upstream 100 bases:

>100_bases
TTAGCATCAAATTTTTCACACTAGTCACTATACTAGCCACAAATTCGATAGAACAACCCTACATATAGTCAATACAACCA
AATTAATCTAAGGAGTTAGG

Downstream 100 bases:

>100_bases
TGATTCAGAGAGCCGATATTATCGGCTCTCCCCTCTTCAATTCAGCGCCAAGCATTGACCACTAAATTGCGCGGTGTTGC
TTTACGATCGCAAAACTCAC

Product: alanine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIIGVPKEIKNHEYRVGMIPASVRELVSHGHQVYVETNAGSGIGFSDDDYIAVGASILPTAADVFAKAEMIVKVKEPQAI
ERAMLKEGQILFTYLHLAPDFPQTEELIKSKAVCIAYETVTDNMGRLPLLAPMSEVAGRMSIQAGAQTLEKSRGGSGLLL
GGVPGVAPAKVVVLGGGVVGANAARMAIGLRADVTILDRNIDTLRKLDEEFQGRANVVYSTADAIEKYVLEADLVIGAVL
IPGAAAPKLVTQQHIKRMKPGSAVVDVAIDQGGCFETSHPTTHAEPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IVKLANKGYQKALLEDAGFLKGLNVIHGKVTYKEVAENFGLDYVDPAKAIAMFN

Sequences:

>Translated_374_residues
MIIGVPKEIKNHEYRVGMIPASVRELVSHGHQVYVETNAGSGIGFSDDDYIAVGASILPTAADVFAKAEMIVKVKEPQAI
ERAMLKEGQILFTYLHLAPDFPQTEELIKSKAVCIAYETVTDNMGRLPLLAPMSEVAGRMSIQAGAQTLEKSRGGSGLLL
GGVPGVAPAKVVVLGGGVVGANAARMAIGLRADVTILDRNIDTLRKLDEEFQGRANVVYSTADAIEKYVLEADLVIGAVL
IPGAAAPKLVTQQHIKRMKPGSAVVDVAIDQGGCFETSHPTTHAEPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IVKLANKGYQKALLEDAGFLKGLNVIHGKVTYKEVAENFGLDYVDPAKAIAMFN
>Mature_374_residues
MIIGVPKEIKNHEYRVGMIPASVRELVSHGHQVYVETNAGSGIGFSDDDYIAVGASILPTAADVFAKAEMIVKVKEPQAI
ERAMLKEGQILFTYLHLAPDFPQTEELIKSKAVCIAYETVTDNMGRLPLLAPMSEVAGRMSIQAGAQTLEKSRGGSGLLL
GGVPGVAPAKVVVLGGGVVGANAARMAIGLRADVTILDRNIDTLRKLDEEFQGRANVVYSTADAIEKYVLEADLVIGAVL
IPGAAAPKLVTQQHIKRMKPGSAVVDVAIDQGGCFETSHPTTHAEPTYIVDDVVHYCVANMPGAVARTSTFALNNATLPY
IVKLANKGYQKALLEDAGFLKGLNVIHGKVTYKEVAENFGLDYVDPAKAIAMFN

Specific function: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=350, Percent_Identity=30.2857142857143, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI122939153, Length=350, Percent_Identity=30.2857142857143, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1787887, Length=309, Percent_Identity=30.42071197411, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17550456, Length=348, Percent_Identity=28.448275862069, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39840; Mature: 39840

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGVPKEIKNHEYRVGMIPASVRELVSHGHQVYVETNAGSGIGFSDDDYIAVGASILPT
CEECCCHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCHHHHH
AADVFAKAEMIVKVKEPQAIERAMLKEGQILFTYLHLAPDFPQTEELIKSKAVCIAYETV
HHHHHHHEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEEH
TDNMGRLPLLAPMSEVAGRMSIQAGAQTLEKSRGGSGLLLGGVPGVAPAKVVVLGGGVVG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
ANAARMAIGLRADVTILDRNIDTLRKLDEEFQGRANVVYSTADAIEKYVLEADLVIGAVL
CCHHHEEEECCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHC
IPGAAAPKLVTQQHIKRMKPGSAVVDVAIDQGGCFETSHPTTHAEPTYIVDDVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLPYIVKLANKGYQKALLEDAGFLKGLNVIHGKVTYKEVAENFG
CCCCHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCC
LDYVDPAKAIAMFN
CCCCCCHHHHCCCC
>Mature Secondary Structure
MIIGVPKEIKNHEYRVGMIPASVRELVSHGHQVYVETNAGSGIGFSDDDYIAVGASILPT
CEECCCHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCHHHHH
AADVFAKAEMIVKVKEPQAIERAMLKEGQILFTYLHLAPDFPQTEELIKSKAVCIAYETV
HHHHHHHEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEEH
TDNMGRLPLLAPMSEVAGRMSIQAGAQTLEKSRGGSGLLLGGVPGVAPAKVVVLGGGVVG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCEEC
ANAARMAIGLRADVTILDRNIDTLRKLDEEFQGRANVVYSTADAIEKYVLEADLVIGAVL
CCHHHEEEECCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHC
IPGAAAPKLVTQQHIKRMKPGSAVVDVAIDQGGCFETSHPTTHAEPTYIVDDVVHYCVAN
CCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLPYIVKLANKGYQKALLEDAGFLKGLNVIHGKVTYKEVAENFG
CCCCHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCC
LDYVDPAKAIAMFN
CCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA