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The map label for this gene is nhaB

Identifier: 227082031

GI number: 227082031

Start: 1986569

End: 1988161

Strand: Direct

Name: nhaB

Synonym: VCM66_1825

Alternate gene names: 227082031

Gene position: 1986569-1988161 (Clockwise)

Preceding gene: 227082029

Following gene: 227082032

Centisome position: 68.68

GC content: 46.96

Gene sequence:

>1593_bases
ATGCCGATGTCCCTCGGAAACGCGTTTATCAAGAATTTCCTTGGTAAAGCGCCTGATTGGTACAAAGTTGCCATCATTGC
TTTTCTTATTATTAACCCAATAGTGTTCTTCTTTATTAATCCCTTCCTCGCGGGTTGGTTATTAGTGGTTGAGTTTATTT
TTACTCTGGCCATGGCACTGAAATGCTACCCTCTACAACCGGGTGGTTTACTTGCGATTGAAGCGATTGCGATTGGGATG
ACAAGTCCGGGGCAAGTTAAGCATGAACTGGTTGCCAATATCGAGGTATTGCTACTGTTGGTGTTTATGGTGGCAGGCAT
CTATTTTATGAAGCAACTGCTGCTGTTTATTTTCACTAAAATTCTGCTTGGCATTCGCTCCAAAGTTCTCCTTTCGATAG
CCTTTAGTGTTACGGCAGCGTTTCTCTCTGCCTTCCTAGATGCGCTGACAGTGATTGCGGTCATCATCAGTGTGGCGGTG
GGTTTCTACTCGATTTATCACAAGGTTGCTTCGGGTCAAAGTGTTCACTCTTCTCACGATCACACCCATGATGAAGGTAT
TTCAGAATTAACTCGTGATGACTTAGAAAACTATCGTGCATTTTTACGTTCACTGCTGATGCACGCAGGTGTGGGTACAG
CACTGGGTGGTGTCACCACTATGGTCGGTGAACCACAAAACCTGATCATTGCTGACCAAGCAGGCTGGCAATTTGGTGAG
TTCCTACTCCGCATGGCCCCCGTTACCGTACCCGTGTTTATTGCAGGTATGCTGACCTGTATGTTGGTTGAGAAATTCCG
CATTTTTGGCTACGGAGCTCGTCTTCCTGCCAATGTTCGTCAAATTCTGCTCGATTTTGACTCAGAAGAGCGCAAAAACC
GCACAAACCAAGACGTTGCCAAACTCTGGGTGCAAGGTGCTATCGCGGTTTGGTTGATTGTGGGCTTAGCTCTTCACTTA
GCCGCCGTTGGTCTTATTGGTTTGTCGGTGATTATTCTCGCCACAGCTTTTACTGGTGTGATTGAAGAACATTCGCTAGG
TAAAGCGTTTGAAGAAGCGCTACCGTTTACCGCATTGCTGGCGGTTTTCTTCTCGATTGTTGCAGTTATTATCGACCAAG
AGCTGTTCAAACCCGTGATTGATGCGGTACTAAATGTCGAAGACCATGGCACTCAGCTCGCACTCTTCTATGTGGCGAAT
GGTCTACTTTCCATGGTGTCAGACAACGTGTTTGTGGGGACGGTTTACATCACTGAAGTAAAAACCGCACTGATGGAAGG
CATGATTTCACGTGACCAATTTGATTTGTTGGCCGTCGCCATCAATACCGGCACCAACTTACCTTCAGTAGCCACACCGA
ACGGTCAAGCGGCCTTCTTATTCTTGCTCACTTCGGCTTTGGCGCCACTAATCCGTCTCTCTTACGGTCGTATGGTCATC
ATGGCGTTTCCTTATACGCTGGCGCTTTCACTGGTTGGTTTTATTGGCATCATGTTCTTACTTGAGCCAATGACTGAAGT
GTTCTATAGCCTAGGTTGGATCAGCCATCATGTCGCGCCAGCTGCAGATGCACTACTGCAAAGTGGCCACTAA

Upstream 100 bases:

>100_bases
CACTGTTGAAAAGATTTTGTCTACACACTGAGTCTCATGCTTTGGTGAAAGCAGGTACGACACAAAGTCTATCTATAACA
ACAGTTAAAGAGTAATCATC

Downstream 100 bases:

>100_bases
ATTTCAATTGAAACTGAAACTAATCCAAGATACAAAGCTCTGAGTATTCAGAGCTTTTCTTTTTAACAAGGATCTATCTG
TGCGTATATTATCCTCCCTT

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MPMSLGNAFIKNFLGKAPDWYKVAIIAFLIINPIVFFFINPFLAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGM
TSPGQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKVLLSIAFSVTAAFLSAFLDALTVIAVIISVAV
GFYSIYHKVASGQSVHSSHDHTHDEGISELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWQFGE
FLLRMAPVTVPVFIAGMLTCMLVEKFRIFGYGARLPANVRQILLDFDSEERKNRTNQDVAKLWVQGAIAVWLIVGLALHL
AAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLNVEDHGTQLALFYVAN
GLLSMVSDNVFVGTVYITEVKTALMEGMISRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVI
MAFPYTLALSLVGFIGIMFLLEPMTEVFYSLGWISHHVAPAADALLQSGH

Sequences:

>Translated_530_residues
MPMSLGNAFIKNFLGKAPDWYKVAIIAFLIINPIVFFFINPFLAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGM
TSPGQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKVLLSIAFSVTAAFLSAFLDALTVIAVIISVAV
GFYSIYHKVASGQSVHSSHDHTHDEGISELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWQFGE
FLLRMAPVTVPVFIAGMLTCMLVEKFRIFGYGARLPANVRQILLDFDSEERKNRTNQDVAKLWVQGAIAVWLIVGLALHL
AAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLNVEDHGTQLALFYVAN
GLLSMVSDNVFVGTVYITEVKTALMEGMISRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVI
MAFPYTLALSLVGFIGIMFLLEPMTEVFYSLGWISHHVAPAADALLQSGH
>Mature_529_residues
PMSLGNAFIKNFLGKAPDWYKVAIIAFLIINPIVFFFINPFLAGWLLVVEFIFTLAMALKCYPLQPGGLLAIEAIAIGMT
SPGQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTKILLGIRSKVLLSIAFSVTAAFLSAFLDALTVIAVIISVAVG
FYSIYHKVASGQSVHSSHDHTHDEGISELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWQFGEF
LLRMAPVTVPVFIAGMLTCMLVEKFRIFGYGARLPANVRQILLDFDSEERKNRTNQDVAKLWVQGAIAVWLIVGLALHLA
AVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALLAVFFSIVAVIIDQELFKPVIDAVLNVEDHGTQLALFYVANG
LLSMVSDNVFVGTVYITEVKTALMEGMISRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVIM
AFPYTLALSLVGFIGIMFLLEPMTEVFYSLGWISHHVAPAADALLQSGH

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family

Homologues:

Organism=Escherichia coli, GI1787435, Length=515, Percent_Identity=68.5436893203884, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAB_VIBC3 (A5F6Z3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F6Z3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1491
- eggNOG:   COG3067
- HOGENOM:   HBG296776
- OMA:   CFLVEKF
- ProtClustDB:   PRK09547
- BioCyc:   VCHO345073:VC0395_A1491-MONOMER
- HAMAP:   MF_01599
- InterPro:   IPR004671
- TIGRFAMs:   TIGR00774

Pfam domain/function: PF06450 NhaB

EC number: NA

Molecular weight: Translated: 57662; Mature: 57531

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x27913b98)-; HASH(0x27bb410c)-; HASH(0x27b16b18)-; HASH(0x279d392c)-; HASH(0x40a22db8)-; HASH(0x40a69ab0)-; HASH(0x27b079c0)-; HASH(0x278d8d1c)-; HASH(0x27ac83d4)-; HASH(0x27bd65c0)-; HASH(0x27bba5e0)-; HASH(0x270a27f4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
PMSLGNAFIKNFLGKAPDWYKVAIIAFLIINPIVFFFINPFLAGWLLVVEFIFTLAMAL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCYPLQPGGLLAIEAIAIGMTSPGQVKHELVANIEVLLLLVFMVAGIYFMKQLLLFIFTK
HHCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLGIRSKVLLSIAFSVTAAFLSAFLDALTVIAVIISVAVGFYSIYHKVASGQSVHSSHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
HTHDEGISELTRDDLENYRAFLRSLLMHAGVGTALGGVTTMVGEPQNLIIADQAGWQFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
FLLRMAPVTVPVFIAGMLTCMLVEKFRIFGYGARLPANVRQILLDFDSEERKNRTNQDVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHCCCCHHHHH
KLWVQGAIAVWLIVGLALHLAAVGLIGLSVIILATAFTGVIEEHSLGKAFEEALPFTALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AVFFSIVAVIIDQELFKPVIDAVLNVEDHGTQLALFYVANGLLSMVSDNVFVGTVYITEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
KTALMEGMISRDQFDLLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVI
HHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
MAFPYTLALSLVGFIGIMFLLEPMTEVFYSLGWISHHVAPAADALLQSGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA