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The map label for this gene is phrB-1

Identifier: 227081943

GI number: 227081943

Start: 1891179

End: 1892564

Strand: Direct

Name: phrB-1

Synonym: VCM66_1737

Alternate gene names: 227081943

Gene position: 1891179-1892564 (Clockwise)

Preceding gene: 227081939

Following gene: 227081944

Centisome position: 65.38

GC content: 50.58

Gene sequence:

>1386_bases
TTGAGTAAAAAAATTGGCCTGTACTGGTTTACCAACGATTTACGAGTGAACGATAACCCGCTACTTGAGCAAGCCTCGCA
GCAAGTGGATCGCTTGATCTGTTTGTACTGTTATCCCTCGATTACGCCATTTTTGGCGCGCTACGCCCAGCAAACACAAT
GGGGCGAAGCCAAAAAAAGGTTTCTCAACCAAACCTTGGCTGACTTAGATCATTCGCTCAGTACGCTAGGCCAAAAGCTT
TGGGTGACGCCACTTTTGCCCTATCAAGCGCTGCGCCATCTACTGACTCAAGTCGAGATTACGGATATTTATGTGGATGC
AGTGGCGGGCTCAGATGAACGCCAAGCTATCGCGCGCATTCATCAGGACTTTTCTTCTGTGCACATCCATCAGCAAGCTC
TGCACAGCTTATTGAGTGAGCCGCAGTTGCCGTTTGCACTTGAGGCGCTACCGAGCACCTTTACCCAATTTCGTAAGCAA
GTAGAAACGATCTCCCTCTCTGCGCCGATGGGCTATCCGCATGTTTTGCCACCCATTGAGCAAGGCTGGCAGTTGCCTTT
GATGGATATTGTTACCGAGCCGAATCACAGCGCCTTTGTCGGTGGTGAGCAAGCAGGGCTGACGCATTGCCAAAACTATT
TCTCCTCCCTGCTCCCCAGTCGCTATAAAGAGACGCGCAATGGGTTAGACGGCATGGATTATTCCACTAAGTTTTCGCCT
TGGCTCGCGCTCGGCGCGGTTTCGCCAAAAACCATTTATGCCATGTTGCAGCGTTATGAAGCTGTACATGGCGCGAACGA
TTCCACCTACTGGATTTTCTTTGAGTTACTGTGGCGTGAATATTTTTACTGGTACGCGCGGCGCTATGGCGCAAAACTGT
TTCGCTTCTCTGGGATTGGCGAGAAAAAACCGCTCACCAGTTTCTATGCGCAGCGCTTTTTACAGTGGAAGCACGGAGAA
ACGCCTTTCCCCATTGTGAATGCCTGTATGCGGCAACTGAATCAAACGGGTTACATGTCCAACCGTGGTCGACAGTTAGT
CGCCAGCTGTCTAGTTCACGAACTAGGTTTAGATTGGCGCTATGGTGCCGCTTATTTTGAAACCCAGTTAGTGGATTACG
ATGTCGGATCCAACTGGGGCAACTGGCAATATTTGGCGGGAGTCGGAGCCGACCCACGCGGCTCGCGGCAATTCAACCTC
GAAAAACAGGCGCACACTTACGATCCAAAGGGCGAGTTCGTTGCCAAATGGTGCGGAACGGCTTGTGACAAGCTAAATGC
TCTCGAAAATTTGGCGCTCGACAGTGTCGATATGGTTGACTGGCCAATCGCCGCCAGCGCGTATCTTCTTATCCATCATC
CTCAAAATAAGGAAAGCTCATCATGA

Upstream 100 bases:

>100_bases
CACTCTCTTTTCGAGGATCAATCCCATTACCGCATGATCGCTCAATCCCTTTGACCGTATAAACGCTCATTAACCCCATC
ATCGGTATTGAGAGTGGAAC

Downstream 100 bases:

>100_bases
ATATCGTGATCATCGGTGGCAGCGGCGGTATTGGTTTGGCGATGGTGCAAGAAGCGCTGAACCGTTTTAGTGAGGCCAAC
GTGCATGCGACTTACCATAG

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSKKIGLYWFTNDLRVNDNPLLEQASQQVDRLICLYCYPSITPFLARYAQQTQWGEAKKRFLNQTLADLDHSLSTLGQKL
WVTPLLPYQALRHLLTQVEITDIYVDAVAGSDERQAIARIHQDFSSVHIHQQALHSLLSEPQLPFALEALPSTFTQFRKQ
VETISLSAPMGYPHVLPPIEQGWQLPLMDIVTEPNHSAFVGGEQAGLTHCQNYFSSLLPSRYKETRNGLDGMDYSTKFSP
WLALGAVSPKTIYAMLQRYEAVHGANDSTYWIFFELLWREYFYWYARRYGAKLFRFSGIGEKKPLTSFYAQRFLQWKHGE
TPFPIVNACMRQLNQTGYMSNRGRQLVASCLVHELGLDWRYGAAYFETQLVDYDVGSNWGNWQYLAGVGADPRGSRQFNL
EKQAHTYDPKGEFVAKWCGTACDKLNALENLALDSVDMVDWPIAASAYLLIHHPQNKESSS

Sequences:

>Translated_461_residues
MSKKIGLYWFTNDLRVNDNPLLEQASQQVDRLICLYCYPSITPFLARYAQQTQWGEAKKRFLNQTLADLDHSLSTLGQKL
WVTPLLPYQALRHLLTQVEITDIYVDAVAGSDERQAIARIHQDFSSVHIHQQALHSLLSEPQLPFALEALPSTFTQFRKQ
VETISLSAPMGYPHVLPPIEQGWQLPLMDIVTEPNHSAFVGGEQAGLTHCQNYFSSLLPSRYKETRNGLDGMDYSTKFSP
WLALGAVSPKTIYAMLQRYEAVHGANDSTYWIFFELLWREYFYWYARRYGAKLFRFSGIGEKKPLTSFYAQRFLQWKHGE
TPFPIVNACMRQLNQTGYMSNRGRQLVASCLVHELGLDWRYGAAYFETQLVDYDVGSNWGNWQYLAGVGADPRGSRQFNL
EKQAHTYDPKGEFVAKWCGTACDKLNALENLALDSVDMVDWPIAASAYLLIHHPQNKESSS
>Mature_460_residues
SKKIGLYWFTNDLRVNDNPLLEQASQQVDRLICLYCYPSITPFLARYAQQTQWGEAKKRFLNQTLADLDHSLSTLGQKLW
VTPLLPYQALRHLLTQVEITDIYVDAVAGSDERQAIARIHQDFSSVHIHQQALHSLLSEPQLPFALEALPSTFTQFRKQV
ETISLSAPMGYPHVLPPIEQGWQLPLMDIVTEPNHSAFVGGEQAGLTHCQNYFSSLLPSRYKETRNGLDGMDYSTKFSPW
LALGAVSPKTIYAMLQRYEAVHGANDSTYWIFFELLWREYFYWYARRYGAKLFRFSGIGEKKPLTSFYAQRFLQWKHGET
PFPIVNACMRQLNQTGYMSNRGRQLVASCLVHELGLDWRYGAAYFETQLVDYDVGSNWGNWQYLAGVGADPRGSRQFNLE
KQAHTYDPKGEFVAKWCGTACDKLNALENLALDSVDMVDWPIAASAYLLIHHPQNKESSS

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor. Has no photolyase activity. Upon purification from either V.cholerae or E.coli an approximately 60 nucleotide RNA is associated with the protein

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain

Homologues:

Organism=Homo sapiens, GI4758072, Length=413, Percent_Identity=26.3922518159806, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI188536100, Length=405, Percent_Identity=24.9382716049383, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI188536103, Length=351, Percent_Identity=24.5014245014245, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786926, Length=436, Percent_Identity=27.0642201834862, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324962, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137248, Length=444, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585455, Length=444, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CRYD_VIBCH (Q9KR33)

Other databases:

- EMBL:   AE003852
- PIR:   B82155
- RefSeq:   NP_231448.1
- ProteinModelPortal:   Q9KR33
- GeneID:   2613694
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1814
- TIGR:   VC_1814
- HOGENOM:   HBG693486
- OMA:   GAEWFES
- ProtClustDB:   CLSK874544
- GO:   GO:0006350
- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00147
- TIGRFAMs:   TIGR02765

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7; SSF52425 DNA_photolyase_N; SSF48173 Photolyase_FAD-bd/Cryptochr_C

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 52736; Mature: 52605

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIGLYWFTNDLRVNDNPLLEQASQQVDRLICLYCYPSITPFLARYAQQTQWGEAKKR
CCCCEEEEEEECCEEECCCHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLNQTLADLDHSLSTLGQKLWVTPLLPYQALRHLLTQVEITDIYVDAVAGSDERQAIARI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
HQDFSSVHIHQQALHSLLSEPQLPFALEALPSTFTQFRKQVETISLSAPMGYPHVLPPIE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHH
QGWQLPLMDIVTEPNHSAFVGGEQAGLTHCQNYFSSLLPSRYKETRNGLDGMDYSTKFSP
CCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCC
WLALGAVSPKTIYAMLQRYEAVHGANDSTYWIFFELLWREYFYWYARRYGAKLFRFSGIG
HHEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EKKPLTSFYAQRFLQWKHGETPFPIVNACMRQLNQTGYMSNRGRQLVASCLVHELGLDWR
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
YGAAYFETQLVDYDVGSNWGNWQYLAGVGADPRGSRQFNLEKQAHTYDPKGEFVAKWCGT
CCHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHH
ACDKLNALENLALDSVDMVDWPIAASAYLLIHHPQNKESSS
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SKKIGLYWFTNDLRVNDNPLLEQASQQVDRLICLYCYPSITPFLARYAQQTQWGEAKKR
CCCEEEEEEECCEEECCCHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLNQTLADLDHSLSTLGQKLWVTPLLPYQALRHLLTQVEITDIYVDAVAGSDERQAIARI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
HQDFSSVHIHQQALHSLLSEPQLPFALEALPSTFTQFRKQVETISLSAPMGYPHVLPPIE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHH
QGWQLPLMDIVTEPNHSAFVGGEQAGLTHCQNYFSSLLPSRYKETRNGLDGMDYSTKFSP
CCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCC
WLALGAVSPKTIYAMLQRYEAVHGANDSTYWIFFELLWREYFYWYARRYGAKLFRFSGIG
HHEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EKKPLTSFYAQRFLQWKHGETPFPIVNACMRQLNQTGYMSNRGRQLVASCLVHELGLDWR
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
YGAAYFETQLVDYDVGSNWGNWQYLAGVGADPRGSRQFNLEKQAHTYDPKGEFVAKWCGT
CCHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHH
ACDKLNALENLALDSVDMVDWPIAASAYLLIHHPQNKESSS
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301